Determination of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection

Quintana, José Benito; Carpinteiro, J.; Rodrı́guez, I.; Lorenzo, R.A.; Carro, A.M.; Cela, R.

doi:10.1016/j.chroma.2003.10.074

Cited by 185 publications

(108 citation statements)

References 24 publications

(33 reference statements)

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“…Therefore, the pH condition was optimized from pH 3 to pH 4. Previous studies also showed that better retention could be acquired by acidified water sample (Ternes et al 1999;Isobe et al 2003;Quintana et al 2004;Bila et al 2007).…”

Section: Resultsmentioning

confidence: 92%

“…The eluting reagent was optimized to ensure a better recovery of target compound and less matrix disrupting. Quintana et al (2004) reported 70-107% of recovery could be obtained using ethyl acetate as elution, and 80-109% of recovery was realized by mixture elution of tert-butyl methyl ether and methanol (1/9, v/v) (Jeannot et al 2002). Therefore, comparing the two-elution strategies, mixture solvent of tert- …”

Section: Solventmentioning

confidence: 99%

See 1 more Smart Citation

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Zhou

et al. 2008

Environ Monit Assess

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A procedure using pre-column trimethylsilyl derivatization and gas chromatography/ mass spectrometry (GC/MS) was developed and applied in determining trace estrogens in complex matrix. Main conditions were optimized, including pH value, salinity of water sample, elution reagents, clean procedure, derivative solvent and temperature. The optimized method was used to determine steroid estrogens in surface water and effluents of wastewater treatment plant (WWTP). Low detection limits of 0.01, 0.03, 0.03, 0.07, 0.09 and 0.13 ng/l for DES, E1, E2, EE2, E3 and E V , respectively were obtained under optimism condition. No apparent interferences appeared in chromatography in comparison with ultrapure water blank. Mean recovery ranged from 72.6% to 111.0% with relative standard deviation of

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Section: Resultsmentioning

confidence: 92%

Section: Solventmentioning

confidence: 99%

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Zhou

et al. 2008

Environ Monit Assess

View full text Add to dashboard Cite

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“…For measurement of E2, the analysis was performed according to the methods of Quintana et al (2004). Briefly, filtered water samples were spiked with deuterated standards, and extracted using preconditioned solid-phase extraction (SPE) cartridges.…”

Section: Analytical Chemistrymentioning

confidence: 99%

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

2009

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a b s t r a c tIt is supposed that the estrogenic effects of estrogenic chemicals will be balanced by the presence of other endocrine-disrupting chemicals (EDCs) with different properties. However, little convincing research into this issue has been carried out to date. In the present study, the combined effects of EDCs with estrogenic and antiestrogenic activities were assessed using a 21-day reproduction assay with the Japanese medaka (Oryzias latipes) as an in vivo model. Natural estrogen (17␤-estradiol, E2) and the pharmaceutical substances letrozole and tamoxifen, which are both model antiestrogens, were tested as reference compounds individually and in combination. Exposure to E2 (200 ng/L) alone resulted in significant biological changes in paired fish, including impaired reproductive capacity and plasma vitellogenin induction. Upon coexposure with tamoxifen or letrozole (10, 50 and 250 g/L), the effects of estrogen on some biomarkers (e.g., plasma vitellogenin concentration) could be neutralized to some extent, but the impairments in reproductive performance were hardly ameliorated or even became more severe. Since reproductive performance is a more holistic parameter with population-level relevance, the possibility that the effects of estrogenic chemicals can be cancelled out by the presence of antiestrogens in aquatic organisms should be questioned. Our observations also illustrate the importance of considering multiple endpoints at different biological levels of organisms for accurate and adequate assessment of the integrated effects of EDCs, especially in environmentally relevant exposure situations.

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“…Several pretreatment methods, such as liquid-liquid extraction (LLE) [17,33], solid-phase extraction (SPE) [3][4][5][6][7][8][23][24][25][26][27][28][29][30][31][32] and stir bar sorptive extraction (SBSE) [10][11][12], have been widely applied to the extraction of EDCs from environmental waters. However, the LLE method demands complex extraction and cleanup procedures, as well as large volumes of sample and organic extractant.…”

Section: Introductionmentioning

confidence: 99%

Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography

Zhou

et al. 2006

Journal of Chromatography A

123

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Determination of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection

Cited by 185 publications

References 24 publications

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography

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