Determination of Methyl Parathion in Water and Its Removal on Zirconia Using Optical Enzyme Assay

Abstract

A simple, miniaturized microplate chemiluminescence assay for determination of methyl parathion (MP) was developed in 384-microwell plates. Zirconia (ZrO(2)) was added in microwell for adsorption of acetylcholinesterase (AChE). The developed assay is based on inhibition of AChE by MP. A good dynamic range 0.008-1,000 ng/mL was obtained for MP with limit of detection 0.008 ng/mL. Intrabatch and interbatch reproducibility for miniaturized assay was obtained with % RSD up to 3.07 and 5.66, respectively. In 384 well plate formats, 70 samples were simultaneously analyzed within 20 min with assay volume of 41.5 μL. The application of developed assay was extended for MP remediation. Column containing ZrO(2) was utilized for remediation where MP was selectively adsorbed. Under optimized condition, adsorption of MP on ZrO(2) was found to be 98-99% with 2-h contact time in real water samples. Adsorption of MP on ZrO(2) column followed by quantification using developed bioassay provides a novel approach to monitor remediation. The applicability of assay was successfully extended for determination of MP in water samples after removal through ZrO(2).

Automated flow based biosensor for quantification of binary organophosphates mixture in milk using artificial neural network

“…In view of the major concerns regarding the toxicity of these compounds, early and simple detection of OP compounds is very important for protecting water resources and food supplies [4] such as milk. However, overuse of these pesticides result in pesticide residues in food [4,5] water [6,7] and environment [8], and leads to a severe threat to human health due to their high toxicity to AChE, which is essential for the functioning of the central nervous system in humans [9].…”

Conception of trapping cidal spray constituents using blend masks in agricultural practices within western region of India

“…The canonical smiles of chemicals were obtained from PubChem database (Kim et al, 2019) [3] , canonical smiles of chemicals were submitted in Swiss target prediction web server and the list of target proteins were retrieved. The proteins having low resolution value were selected for each chemical from RCSB PDB (Chen et al, 2007) [2] .…”

“…Pesticides are designed to kill and because their mode of action is not specific to one species, they often kill or harm organisms other than pests, including humans (Mori et al, 2010) [5] . Use of large amounts of pesticides has raised human health and ecological concerns (Deshpande et al, 2011) [3] . Over the past three decades, the indiscriminate use and improper handling of pesticides in agriculture have caused serious human health problems in many developing countries.…”