Determination of methotrexate and folic acid by ion chromatography with electrochemical detection on a functionalized multi-wall carbon nanotube modified electrode

Sensitive Voltammetric Sensor Based on Boron‐Doped Diamond Electrode for Determination of the Chemotherapeutic Drug Methotrexate in Pharmaceutical and Biological Samples

“…It is often combined with solid phase extraction [5,6] and with various types of detection systems, e.g., fluorimetric detector [7][8][9], UV spectrophotometric detector [10], mass spectrometer [6,11,12] or electrochemical detector [13]. Other methods, which have been successfully used for MTX determination, are ion chromatography with electrochemical detection [14] and capillary zone electrophoresis [15,16]. Spectrophotometric methods including color reactions and UV measurements have also been reported [17][18][19].…”

Electrochemical Monitoring of Methotrexate Anticancer Drug in Human Blood Serum by Using in situ Solvothermal Synthesized Fe3O4/ITO Electrode

“…Figure c showed the SWVs of different concentrations of MTX at Fe 3 O 4 /ITO electrode that revealed a broad oxidation peak at −0.37 V, and the current peak was increased with increasing the MTX concentration. The relationship between the current peak and the MTX concentrations showed a linear relationship within a range from 10 −8 to 10 −14 M (Figure d) with a LOD of 0.4×10 −15 M. The electrochemical activity of our modified electrode was compared with some other electrodes previously reported for the electrochemical determination of MTX (Table ), which revealed that our modified electrode owned a lower LOD and a wider linear range for MTX determination in comparing with most previously reported electrodes . Thus SWV was used for further electrochemical measurements.…”

“…But, HPLC and CE are expensive instruments; also using fluorescence spectroscopy process needs complicated sample preparation processes, which limited their clinical applications. Electrochemical techniques have a lot of advantages like high sensitivity, selectivity, simplicity, low-cost, rapid detection, and ease of on-site determination, thus several modified electrodes have been designed for electrochemical MTX detection [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26].…”

Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

“…Other strategies that have been proposed for MTX sensing include methods based on electrochemistry, [16][17][18][19][20][21] nanoparticlebased optical detection [22][23][24] or bioluminescence. 25 We recently introduced a colorimetric test for MTX based on a competitive assay involving folic acid-derived Au NP and human dihydrofolate reductase (hDHFR).…”