Determination of methamphetamine in neonatal hair and meconium samples: estimation of fetal drug abuse during pregnancy

Abstract

Determination of methamphetamine (MP) and its metabolite amphetamine (AP) in hair and meconium samples from a neonate was performed to estimate the fetal abuse of MP during pregnancy. Five hundred micrograms of the neonate's hair spiked with 1-methyl-3-phenylpropylamine (internal standard) were used for the determination. After digestion with 1 M NaOH for 20 min, the resultant was extracted with heptane and the organic layer was evaporated and labeled with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole. The MP in 10 mg of the neonate's meconium was determined using the standard addition method. The determination of MP and AP in the hair was performed by a semi-micro-HPLC-peroxyoxalate chemiluminescence method. The hair sample (total length = ca. 3 cm) was divided into two fragments (the root and the tip). The concentrations of MP in the fragments were 1.68 ± 0.18 ng/mg (root) and 1.29 ± 0.03 ng/mg (tip). AP was not found in either of the fragments. The concentrations of MP and AP in meconium, based on measurements performed in triplicate, were 0.27 ± 0.04 ng/mg and 0.16 ± 0.03 ng/mg, respectively. As a result, fetal abuse of MP during the pregnancy was confirmed.

Consequências à criança do uso de drogas durante a gestação: um artigo de revisão

“…4,15,13,14,18,19 Em estudo comparativo entre os dois métodos mais utilizados (análise de mecônio e análise de cabelo materno), concluiuse que a análise de cabelo materno parece ser mais sensível que a análise em mecônio, principalmente para maconha. García-Serra et al em seu estudo observaram que, dos 11 em mecônio e, dos 7 casos positivos para cocaína, 6 foram 4 Em estudo de análise em cabelo materno, Friguls et al não encontraram casos positivos em entrevista que resultassem negativos por análise.…”

Clinical Applications of Hair Analysis

“…Nakamura . Wada et al performed neonatal hair analysis in a low birth weight neonate whose mother was suspected of using MA during her pregnancy [55]. Wada et al performed neonatal hair analysis in a low birth weight neonate whose mother was suspected of using MA during her pregnancy [55].…”

Wartość Diagnostyczna Włosów

“…W wyniku tego programu zaobserwowano, że metamfetamina także przechodzi przez łożysko oraz że nie u wszystkich matek biorących kokainę przeszła ona przez łożysko do dziecka [33]. Zbadano także zawartość metamfetaminy i amfetaminy w smółce i włosach [34]. Te wyniki traktowane kompleksowo stanowią dowód zażywania w ciąży narkotyków.…”