Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring

Kamao, Maya; Hirota, Yoshio; Suhara, Yoshitomo; Tsugawa, Naoko; Nakagawa, Kimie; Okano, Toshio; Hasegawa, Hiroshi

doi:10.2116/analsci.33.863

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Thanks to the development of mass spectrometry (MS) technologies, especially coupling it to high-performance liquid chromatography (LC-MS) and tandem MS (MS/MS) in the recent years, researchers can successfully determine various analytes in different samples. [8][9][10][11] For the oxidized phospholipids, there have been reports on the determination of different kinds of oxidized phospholipids in various biological samples, including the hydroperoxides of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and cardiolipin (CL), as well as PC aldehydes (PC-CHO). [12][13][14][15][16][17][18] Nevertheless, with regard to lipidomics in diabetes, although there have been studies on lipidomics via LC-MS, 19 there are seldom reports concerning on the changes of these oxidized phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

Chen

Zang

et al. 2018

ANAL. SCI.

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Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.

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Section: Introductionmentioning

confidence: 99%

Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

Chen

Zang

et al. 2018

ANAL. SCI.

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“…Quantification of menadione in human urine samples is challenging since it has low molecular weight and is unlikely to produce stable multiple reaction monitoring (MRM)-suitable fragments. Kamao et al overcame these obstacles while determining menadione in urine, rat plasma, cultured cell and media by using pseudo MRM based LC-MS/MS method (70). HPLC-FD method has been developed by Al Rajebi group to measure urinary manadione to illuminate the role of menadione in MK-4 formation process (39).…”

Section: Human Urinementioning

confidence: 99%

A concise review of quantification methods for determination of vitamin K in various biological matrices

Zhang

Bala

Mao

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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Vitamin K is an essential nutrient in the body and involved in numerous physiological and pathophysiological functions. Both the lack and surplus of vitamin K can put human health at risk. Therefore, it becomes necessary to monitor vitamin K concentrations in different biomatrices through establishing sensitive and specific analytical methods. This review collectively describes an updated overview of the sample pretreatment methodologies and methods for quantitative determination of vitamin K that have been used in last two decades. High Performance Liquid Chromatography (HPLC) is commonly utilized as a standard for separation of vitamin K in combination with different detection including spectroscopic, spectrometric, fluorometric and mass spectroscopy. Recent progress in sample pretreatment technologies and quantitation methodologies have enhanced the ability to identify and quantitate vitamin K in biomatrices to further advance our understanding of the role of this vitamin in human health and disease.

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“…Liquid chromatography (LC) coupled to ultraviolet, ,,− and fluorescence − detection, the latter involving a derivatization step, has been used for vitamin K analysis in a wide variety of samples. The literature also shows the coupling of LC separations to MS and tandem MS (MS/MS) with atmospheric pressure chemical ionization (APCI) ,,,− and electrospray ionization (ESI), ,− with both ionization sources in positive mode.…”

Section: Introductionmentioning

confidence: 99%

Microwave Assisted Cloud Point Extraction for the Determination of Vitamin K Homologues in Vegetables by Liquid Chromatography with Tandem Mass Spectrometry

Campillo

Marín

Viñas

et al. 2019

J. Agric. Food Chem.

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Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) with a triple quadrupole (QqQ) is proposed for determining the vitamin K homologues, phylloquinone (PK), menaquinone-4 (MK) and menadione (MD), in vegetables. The analytes were isolated from the samples (1−1.5 g) by ultrasound assisted extraction using acetonitrile (2 mL), and the liquids were submitted to microwave assisted cloud point extraction with Triton X-45. The enrichment factors were between 20 and 50, depending on the vitamin homologue in question. The analytes were determined by LC-ESI-QqQ-MS/MS in the multiple reaction monitoring (MRM) mode, providing unequivocal identification and quantification, with limits of detection of 0.8, 1.0, and 16 ng/g for MK, PK, and MD, respectively. Recovery assays for samples spiked at two concentration levels, between 40 and 600 ng/g depending on the compound, provided recoveries in the 90−114% range. Only PK was detected in the samples analyzed, at concentrations in the 90−2350 ng/g range.

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Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring

Cited by 6 publications

References 23 publications

Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

A concise review of quantification methods for determination of vitamin K in various biological matrices

Microwave Assisted Cloud Point Extraction for the Determination of Vitamin K Homologues in Vegetables by Liquid Chromatography with Tandem Mass Spectrometry

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