Determination of malathion's toxic effect on Lens culinaris Medik cell cycle

Abstract

The present study aimed to determine the toxic effect of malathion pesticide on root growth, cell division and the chromosomal abnormalities frequency using the L. culinaris test. Initially, the lentil seeds were subjected to different doses of malathion (0.0 0.5, 1, 2.5, 5, 10, 15, 20, 25 and 30 mgL-1) and during 24, 48, and 72 h, the root length was measured. Subsequently, at 72h, the mitotic index, mitotic inhibition, and cellular abnormalities were calculated for all treatments. According to the obtained results, it was visualized that the root growth was inversely proportional to the concentration of malathion at all times of exposure. After 72h of exposure, the lowest values of the mitotic index and inhibition were presented at malathion concentrations 20, 25 and 30 mgL-1. Additionally, micronuclei cell abnormalities, metaphase sticky chromosomes, split chromosomes, nuclear lesions, irregular anaphase, anaphase bridges, binucleated cells, absence of nucleus and telophase bridge were observed. Finally, Malathion induced mitodepressive and cytotoxic effects in the meristematic cells of the L. culinaris root tip. A high frequency of abnormality was found in the micronuclei, which represented an indicator of a high degree of toxicity at the cellular level.

Seedling competition between Pennisetum setaceum (Poaceae) and three native weeds of La Primavera wood, Guadalajara, Jalisco (México)

“…Seed viability was quantified using the tetrazolium (2,3,5triphenyltetrazolium chloride) test as described by Maldonado-Peralta et al [21]. For each species, 30 seeds were analysed in petri dishes coated with a 0.1% solution of tetrazolium (2,3,5-triphenyltetrazolium chloride) [22]. These prepared seeds were then incubated in a germination chamber (SEEDBURO, MPG1000) at a controlled temperature of 25ºC in the dark for 24 hours.…”

Paracetamol ecotoxicological bioassay using the bioindicators Lens culinaris Med. and Pisum sativum L

“…The use of bioindicators with plant seeds over the years has been one of the scientific techniques reported for the determination of cytotoxicity, proving to be an instrument of high sensitivity and easy handling (Mendoza and Salazar 2022 ; Mercado and Caleño 2021 ; García-Medina et al 2020 ; Azzazy 2020 ; Leston et al 2013 ; Nikitaki et al 2018 ; Kummerová et al 2013 ). This is how plant species have been used for the identification of cytological changes caused by multiple chemical agents such as Allium cepa , Lens culinaris , and Pisum sativum Salazar and Quintero Caleño ( 2020 ), also recommended for their economic and practical efficiency, considering that the increasing production, the use of this drug, its environmental presence, and the hazardous impact on aquatic organisms and other non-target individuals could increase rapidly. This study evaluates the possible cytotoxic effects of paracetamol using Lens culinaris Med.…”

Cytogenetic Effects of the Neonicotinoid Insecticides Nuprid 200 SL and Calypso 480 SC on Plant Model System

“…One of the possibilities for studying the genotoxic effect of pesticides is using plants as test objects. Plants are recognized as excellent genetic models in detecting mutagenic environmental agents and are often used for biomonitoring studies [12][13][14][15][16].…”