Determination of leukotriene B4 by high-performance liquid chromatography with electrochemical detection

Herrmann, Thomas; Steinhilber, Dieter; Roth, H. J.

doi:10.1016/0378-4347(87)80500-5

Cited by 20 publications

(10 citation statements)

References 14 publications

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“…The electrochemical detector was set at +1.35 V with a 2 sec response time. The minimum detection of o-oxidized products of LTB, (20-OH-LTB4 and 20-COOH-LTB4) was approximately 50 pg, which is consistent with the values of 50-60 pg reported by Herrmann et al (1987).…”

Section: Analysis Of Eicosanoidssupporting

confidence: 89%

“…Both A,,,,,, and FMLP stimulated the production of w-oxidation products of LTB,. PMN incubated with FMLP generate far lower levels of o-products than cells incubated with A23,187, as previously observed by Haurand and Flohe (1989) and Herrmann et al (1987). In contrast, LXA, a t 10-7M did not stimulate detectable levels of w oxidation products of LTB,.…”

Section: U P T a K E And Agonist-induced Release Of [L-'4clarachidonisupporting

confidence: 78%

“…A third RP-HPLC system was employed to detect picogram levels of 20-OH-LTB4 and 20-COOH-LTB4 as described by Herrmann et al (1987Herrmann et al ( ,1988. The column (Altex, Ultrasphere-ODs, 4.6 mm x 25 cm) was eluted with methanol/water (65:35, v/v) with TFA (1 mM) and the UV detector (Waters Assoc.…”

Section: Analysis Of Eicosanoidsmentioning

confidence: 99%

“…extracted, and chromatographed as described under Methods. The o-oxidation products of LTB, were quantitated by ED-UV RP-HPU: as inHerrmann et al (1987). Results are expressed in nglincubation; mean -t SD of three individual experiments where the amounts detected in incubations with vehicle and PMN were subtracted from values obtained with agonists.…”

mentioning

confidence: 99%

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Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Nigam

Fiore

Luscinskas

et al. 1990

Journal Cellular Physiology

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The profiles of actions of lipoxin A4 (LXA4) and lipoxin B4 (LXB4), two lipoxygenase-derived eicosanoids, were examined with human neutrophils. At nanomolar concentrations, LXA4 and LXB4 each stimulated the release of [1-14C]arachidonic acid from esterified sources in neutrophils. Lipoxin-induced release of [1-14C]arachidonic acid was both dose- and time-dependent and was comparable to that induced by the chemotactic peptide f-met-leu-phe. Time-course studies revealed that lipoxin A4 and lipoxin B4 each induced a biphasic release of [1-14C]arachidonic acid, which was evident within seconds (5-15 sec) in its initial phase and minutes (greater than 30 sec) in the second phase. In contrast, the all-trans isomers of LXA4 and LXB4 did not provoke [1-14C]AA release. Lipoxin-induced release of arachidonic acid was inhibited by prior treatment of the cells with pertussis toxin but not by its beta-oligomers, suggesting the involvement of guaninine nucleotide-binding regulatory proteins in this event. Dual radiolabeling of neutrophil phospholipid classes with [1-14C]arachidonic acid and [3H]palmitic acid showed that phosphatidylcholine was a major source of lipoxin-induced release of [1-14C]arachidonic acid. They also demonstrated that lipoxins rapidly stimulate both formation of phosphatidic acid as well as phospholipid remodeling. Although both LXA4 and LXB4 (10(-8)-10(-6) M) stimulated the release of [1-14C]arachidonic acid, neither compound evoked its oxygenation by either the 5- or 15-lipoxygenase pathways (including the formation of LTB4, 20-COOH-LTB4, 5-HETE, or 15-HETE). LXA4 and LXB4 (10(-7) M) each stimulated the elevation of cytosolic Ca2+ as monitored with Fura 2-loaded cells, albeit to a lesser extent than equimolar concentrations of FMLP. Neither lipoxin altered the binding of [3H]LTB4 to its receptor on neutrophils. In addition, they did not stimulate aggregation or induce adhesion of neutrophils to human endothelial cells. Results indicate that both LXA4 and LXB4 stimulate the rapid remodeling of neutrophil phospholipids to release arachidonic acid without provoking either aggregation or the formation of lipoxygenase-derived products within a similar temporal and dose range. Together they indicate that LXA4 and LXB4 display selective actions with human neutrophils and suggest that these eicosanoids possess unique profiles of action which may regulate neutrophil function during inflammation.

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Section: Analysis Of Eicosanoidssupporting

confidence: 89%

Section: U P T a K E And Agonist-induced Release Of [L-'4clarachidonisupporting

confidence: 78%

Section: Analysis Of Eicosanoidsmentioning

confidence: 99%

mentioning

confidence: 99%

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Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Nigam

Fiore

Luscinskas

et al. 1990

Journal Cellular Physiology

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“…Several systems have previously been reported in literature. Predominantly, eicosanoids are quantified in biological samples by immunological methods [11], while HPLC-based assays with UV detection [12], radioisotope detection [13], fluorescence detection [14] or electrochemical detection [15] are applied as well. Unfortunately, most of them suffer from disadvantages, which limit their application as routine screening system.…”

Section: Introductionmentioning

confidence: 99%

Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run

Frohberg

Drutkowski

Wobst

2006

Journal of Pharmaceutical and Biomedical Analysis

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Analysis of leukotrienes by liquid chromatography/electrochemistry after conversion to dinitrobenzoate derivatives

Steffenrud

Salari

1989

Biomedical Chromatography

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A sensitive liquid chromatography method has been developed using electrochemistry for the determination of leukotrienes in biological fluids. Biological specimens are treated with 3,5-dinitrobenzoyl chloride in acetonitrile which undergoes rapid reaction with hydroxyl groups of non-peptidic leukotrienes in the presence of pyridine and with amino groups of peptidic leukotrienes in the presence of potassium tetraborate buffer. The resulting dinitrobenzoate derivatives of leukotrienes are highly electroactive, suitable for reduction or oxidation at moderate potentials by an electrochemical detector. In reductive mode at -0.7 V or oxidative mode at +1.15 V potentials, the lower limits of detection for leukotriene derivatives were approximately 8 +/- 3 pg and 70 +/- 16 pg respectively, with a signal-to-noise ratio of 5 to 1. This method was applied to the detection of leukotrienes in plasma, nasal and bronchial fluids of patients with asthma.

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Determination of leukotriene B4 by high-performance liquid chromatography with electrochemical detection

Cited by 20 publications

References 14 publications

Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run

Analysis of leukotrienes by liquid chromatography/electrochemistry after conversion to dinitrobenzoate derivatives

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Determination of leukotriene B4 by high-performance liquid chromatography with electrochemical detection

Cited by 20 publications

References 14 publications

Lipoxin A4 and lipoxin B4 stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Lipoxin A4 and lipoxin B4 stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run

Analysis of leukotrienes by liquid chromatography/electrochemistry after conversion to dinitrobenzoate derivatives

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Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion

Lipoxin A₄ and lipoxin B₄ stimulate the release but not the oxygenation of arachidonic acid in human neutrophils: Dissociation between lipid remodeling and adhesion