Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run

Frohberg, P.; Drutkowski, G.; Wobst, Ivonne

doi:10.1016/j.jpba.2006.02.046

Cited by 27 publications

(15 citation statements)

References 31 publications

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“…Studies have reported that ozone enhances 15-HETE in tracheal epithelial cells (1). Since multiple enzymes such as cytochrome P-450 and 15-LO enzymes metabolize AA to 15-HETE, indomethacin may have little effect in abrogating the synthesis of 15-HETE (23,65). Plausibly, ozone-mediated augmentation of IL-6 secretion in the absence of PGE 2 is, in part, due to LO metabolites.…”

Section: Discussionmentioning

confidence: 99%

Ozone modulates IL-6 secretion in human airway epithelial and smooth muscle cells

Damera¹,

Zhao²,

Wang³

et al. 2009

American Journal of Physiology-Lung Cellular and Molecular Phys

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Although ozone enhances leukocyte function and recruitment in airways, the direct effect of ozone in modulating structural cell-derived inflammatory mediators remains unknown. Using a coculture model comprised of differentiated human airway epithelial cells (NHBE) and smooth muscle cells (ASM), we postulate that ozone regulates IL-6 secretion in basal and cytokine-primed structural cells. Air-liquid interface (ALI) cultures of NHBE cells underwent differentiation as determined by mucin secretion, transepithelial electrical resistance (TEER), and ultrastructure parameters. Whereas TNF enhanced basal secretion of IL-6 (57 +/- 3%), ozone exposure at 0.6 ppm for 6 h augmented IL-6 levels in basal (41 +/- 3%) and TNF- (50 +/- 5%) primed cocultures compared with that derived from NHBE or ASM monolayers alone. Levels of PGE(2), 6-keto-PGF(1alpha), PGF(2alpha), and thromboxane B(2) (TxB(2)) levels in basal and TNF-primed cocultures revealed that ozone selectively enhanced PGE(2) production in TNF- (6 +/- 3-fold) primed cocultures, with little effect (P > 0.05) on diluent-treated cultures. In accordance with ozone-induced increases in PGE(2) levels, cyclooxygenase inhibition with indomethacin partially abolished IL-6 secretion. Surprisingly, indomethacin had little effect on constitutive secretion of IL-6 in cocultures, whereas indomethacin completely restored ozone-mediated TEER reduction in TNF-primed cocultures. Collectively, our data for the first time suggest a dual role of ozone in modulating IL-6 secretion and TEER outcomes in a PGE(2)-dependent (in presence of TNF stimulus) and -independent manner (in absence of cytokine stimulus).

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Section: Discussionmentioning

confidence: 99%

Ozone modulates IL-6 secretion in human airway epithelial and smooth muscle cells

Damera¹,

Zhao²,

Wang³

et al. 2009

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…5-HETE, LTB 4 , and 12-HHT were identified by comparing their retention time with those of authentic standards and quantified by their peak areas. The relative differences in concentration among samples are quantified by peak areas ratio of samples and PGB 2 (Frohberg et al, 2006).…”

Section: Analyses Of Aa Metabolites By Hplc With Diode Array Detectormentioning

confidence: 99%

The anti-inflammatory activities of an extract and compounds isolated from Platycladus orientalis (Linnaeus) Franco in vitro and ex vivo

Fan

Zeng

Pei

et al. 2012

Journal of Ethnopharmacology

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“…HETE, HODE and HEPE were extracted from rat serum and mammary tumours with solid phase extraction method (SPE), described by Frohberg with some modifications [26,33]. Aliquots of 0.2-0.4 mL of serum samples were diluted with 0.5 mL of methanol and mixed vigorously.…”

Section: Lox Metabolites Analysis In Serum and Mammary Tumoursmentioning

confidence: 99%

The Effect of Diet Supplementation with Pomegranate and Bitter Melon on Lipidomic Profile of Serum and Cancerous Tissues of Rats with Mammary Tumours

et al. 2020

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The aim of this study was to present overall lipid profile of organisms with ongoing neoplastic process and applied diet supplementation with pomegranate seed oil (PSO) and bitter melon extract (BME). The following were quantified in serum and cancerous tissues of rats suffering from mammary tumours: fatty acids, conjugated fatty acids and sterols, their oxidised metabolites (malondialdehyde and oxysterols) and lipoxygenase (LOX) metabolites of polyunsaturated fatty acids. The obtained results indicate that abnormalities in lipid metabolism accompany neoplastic process. These differences concern all classes of lipids and most pathways of their transformation, with the special emphasis on lipid peroxidation and LOX-mediated metabolism. Cancer process appears to be so detrimental that it may conceal positive influence of dietary modifications. The lack of anticarcinogenic properties of PSO and BME in this model may be due to their antioxidant properties or elevated levels of conjugated linoleic acids (CLA), which change CLA isomer activity from anti- to pro-tumorigenic. As CLA are the product of conjugated linolenic acids (CLnA) endogenous metabolism, high CLA levels may be explained by applied diet enrichment.

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Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run

Cited by 27 publications

References 31 publications

Ozone modulates IL-6 secretion in human airway epithelial and smooth muscle cells

Ozone modulates IL-6 secretion in human airway epithelial and smooth muscle cells

The anti-inflammatory activities of an extract and compounds isolated from Platycladus orientalis (Linnaeus) Franco in vitro and ex vivo

The Effect of Diet Supplementation with Pomegranate and Bitter Melon on Lipidomic Profile of Serum and Cancerous Tissues of Rats with Mammary Tumours

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