Determination of lead in whole blood: Comparison of the LeadCare blood lead testing system with Zeeman longitudinal electrothermal atomic absorption spectrometry

Pineau, Alain; Fauconneau, Bernard; Rafael, Marina Neto; Viallefont, Anne; Guillard, Olivier

doi:10.1016/s0946-672x(02)80037-2

Cited by 42 publications

(25 citation statements)

References 8 publications

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“…In a study involving 208 paired samples, the mean difference was 3.41 ± 7.61 µg/dL for ≤ 10 µg/dL values, and 0.57 ± 5.35 µg/dL for > 10 µg/dL values, with a correlation coefficient of 0.819 in BLL in the range from 1 to 42 µg/dL (Taylor et al 2001). Another study of 76 samples with a range of BLL from 1.5 to 53 µg/dL showed a correlation between both methods with an r = 0.95 (Pineau et al 2002).…”

Section: Discussionmentioning

confidence: 93%

Lead Exposure: Pottery Impact in Tlaxcala, Mexico

Ortiz-Ortiz

García-Nieto

Juárez-Santacruz

et al. 2017

Rev. Int. Contam. Ambie.

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The objective of this work was to assess the blood lead level in paired samples of pottery-glaze workers to confirm the LeadCare ® II System (LCS) reliability using this device in the general population, and quantify this metal in cooked food in lead-glazed containers. The blood lead measurement in pottery-glaze workers was carried out by graphite furnace atomic absorption spectrometry (GFAAS) and the LCS. this last method was also used in the general population. The food lead level was quantified through GFAAS. The paired data showed a significant correlation (r = 0.976, p < 0.001), a mean difference of 3.3 µg/dL with limits of agreement between -2.4 to 9 and an intraclass correlation coefficient of 0.928. These results indicate a good concordance between both methods. The blood lead median of 32.0 µg/dL and interquartile range (IQR) of 16.1 was higher in the pottery-glaze workers than in the general population (children = 5.4 µg/dL, IQR = 5.9 and adults = 8.3 µg/dL, IQR = 2.7). Lead in food exceeded the maximum level considered in the Codex alimentarius. Pottery persists as a potential risk in the Tlaxcala State population.Palabras clave: sangre, alimentos, alfareros, niños, sistema LeadCare II RESUMENEl objetivo de este trabajo fue evaluar el nivel de plomo sanguíneo en muestras pareadas de alfareros para confirmar la confiabilidad del sistema LeadCare II (SLC) empleando este dispositivo en la población general, y cuantificar este metal en alimentos cocinados en recipientes de barro vidriado. La medición de plomo en sangre de los alfareros, se realizó por espectrometría de absorción atómica con horno de grafito (EAAHG) y el SLC, este último método fue utilizado también en la población general. El nivel de plomo en alimentos fue cuantificado por EAAHG. Los datos pareados mostraron una correlación significativa (r = 0.976, p < 0.001), una diferencia media de 3.3 µg/dL Rev. Int. Contam. Ambie. 33 (1) 57-64, 2017 DOI: 10.20937/RICA.2017 E. Ortiz-Ortiz et al. 58con límites de concordancia de -2.4 a 9 y un coeficiente de correlación intraclase de 0.928; esos resultados indican concordancia entre los métodos. La mediana de plomo sanguíneo de 32.0 µg/dL y el rango intercuartílico (RIC) de 16.1 fue superior en los alfareros que en la población general (niños = 5.4 µg/dL, RIC = 5.9 y adultos = 8.3 µg/dL, RIC = 2.7). La concentración de plomo en el alimento superó el nivel máximo que contempla el Codex alimentarius. La alfarería persiste como un riesgo potencial en la población Tlaxcalteca.

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Section: Discussionmentioning

confidence: 93%

Lead Exposure: Pottery Impact in Tlaxcala, Mexico

Ortiz-Ortiz

García-Nieto

Juárez-Santacruz

et al. 2017

Rev. Int. Contam. Ambie.

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“…When method differences were plotted against GFAAS mean values, 95% of differences fell within ±11 μg/dL; linear regression yielded an r 2 =0.67. Another study of 76 paired samples comparing LCS to GFAAS [6], reported r=0.95 (r 2 =0.90) overall for blood lead levels between 1.3 and 55 μg/dL; for blood lead values between 0 and 15 μg/dL (n=31) the correlation was substantially lower, r=0.697 (r 2 =0.486). In addition, overall the 76 blood lead levels determined by LCS were significantly lower than those obtained via GFAAS (t=−7.73, p< 0.001).…”

Section: Introductionmentioning

confidence: 99%

A Bland–Altman Comparison of the Lead Care® System and Inductively Coupled Plasma Mass Spectrometry for Detecting Low-Level Lead in Child Whole Blood Samples

et al. 2010

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Chronic childhood lead exposure, yielding blood lead levels consistently below 10 μg/dL, remains a major public health concern. Low neurotoxic effect thresholds have not yet been established. Progress requires accurate, efficient, and cost-effective methods for testing large numbers of children. The LeadCare® System (LCS) may provide one ready option. The comparability of this system to the "gold standard" method of inductively coupled plasma mass spectrometry (ICP-MS) for the purpose of detecting blood lead levels below 10 μg/dL has not yet been examined. Paired blood samples from 177 children ages 5.2-12.8 years were tested with LCS and ICP-MS. Triplicate repeat tests confirmed that LCS and ICP-MS had comparable repeatability. As compared with ICP-MS, LCS had a negative bias of 0.457 μg/dL with an average variability of 1.0 μg/dL. The reproducibility and precision of the LCS is appropriate for the evaluation and monitoring of blood lead levels of individual children in a clinical setting. Recent research however has suggested that increments as small as 0.5 μg/dL may distinguish those at risk of low-level lead-induced neurotoxicity. Thus, we also conclude that the LCS is not useful for research applications attempting to identify neurotoxic effect thresholds for chronic lowest level lead exposure in children. For these types of research applications, a convenient and low-cost device is needed for the precise detection of child blood lead levels below 10 μg/dL.

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“…LeadCare has been used for Pb detection in human medicine and to a limited extent in veterinary medicine. [3][4][5]7,12,13,16 The manufacturer recommends that the blood samples be analyzed within 24 hr of collection.…”

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confidence: 99%

Comparison of Two Methods for Blood Lead Analysis in Cattle: Graphite-Furnace Atomic Absorption Spectrometry and Leadcare® II System

et al. 2010

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Abstract. The current study compared the LeadCareH II test kit system with graphite-furnace atomic absorption spectrometry for blood lead (Pb) analysis in 56 cattle accidentally exposed to Pb in the field. Blood Pb concentrations were determined by LeadCare II within 4 hr of collection and after 72 hr of refrigeration. Blood Pb concentrations were determined by atomic absorption spectrometry, and samples that were coagulated (n 5 12) were homogenized before analysis. There was strong rank correlation (R 2 5 0.96) between atomic absorption and LeadCare II (within 4 hr of collection), and a conversion formula was determined for values within the observed range (3-91 mcg/dl, although few had values .40 mcg/dl). Median and mean blood pb concentrations for atomic absorption were 7.7 and 15.9 mcg/dl, respectively; for LeadCare II, medians were 5.2 mcg/dl at 4 hr and 4.9 mcg/dl at 72 hr, and means were 12.4 and 11.7, respectively. LeadCare II results at 4 hr strongly correlated with 72 hr results (R 2 5 0.96), but results at 72 hr were lower (P , 0.01). There was no significant difference between coagulated and uncoagulated samples run by atomic absorption. Although there have been several articles that compared LeadCare with other analytical techniques, all were for the original system, not LeadCare II. The present study indicated that LeadCare II results correlated well with atomic absorption over a wide range of blood Pb concentrations and that refrigerating samples for up to 72 hr before LeadCare II analysis was acceptable for clinical purposes.

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Determination of lead in whole blood: Comparison of the LeadCare blood lead testing system with Zeeman longitudinal electrothermal atomic absorption spectrometry

Cited by 42 publications

References 8 publications

Lead Exposure: Pottery Impact in Tlaxcala, Mexico

Lead Exposure: Pottery Impact in Tlaxcala, Mexico

A Bland–Altman Comparison of the Lead Care® System and Inductively Coupled Plasma Mass Spectrometry for Detecting Low-Level Lead in Child Whole Blood Samples

Comparison of Two Methods for Blood Lead Analysis in Cattle: Graphite-Furnace Atomic Absorption Spectrometry and Leadcare® II System

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