Determination of lansoprazole in human plasma by rapid resolution liquid chromatography–electrospray tandem mass spectrometry: Application to a bioequivalence study on Chinese volunteers

Wu, Guolan; Zhou, Haiyan; Shentu, Jianzhong; He, Qiaojun; Yang, Bo

doi:10.1016/j.jpba.2008.09.046

Cited by 18 publications

(9 citation statements)

References 10 publications

(16 reference statements)

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“…23 Furthermore, studies confirmed that simvastatin could potently induce mRNAs encoding osteoblast differentiation markers such as BMP-2, ALP, type I collagen, bone sialoprotein, and osteocalcin. 24 Although XRD and FTIR analysis showed the coatings consisted of HA crystals, FSEM observation demonstrated that HA crystals changed as the concentration of drugs. The higher the concentration was, the smaller the diameter of crystals was.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, BMP-2-mediated action was most accepted, as first described by Mundy et al 9 Maeda et al demonstrated that simvastatin initially enhanced BMP-2 expression and then induced augmentation of differentiation markers characteristic of late osteoblast stages, and suppressed gene expression of matrix metallopreinase 13 (collagenase-3) simultaneously. 24 Nitric oxide production through statininduced up-regulated endothelial nitric oxide synthase also played some key roles in regulating osteoblast differentiation and bone formation. 26 And calcyclin (S100A6), a Ca 2+ ion-binding protein, was identified by proteomic analysis to be significantly up-regulated when treated with simvastatin and suggested to play an important role in an anabolic effect of simvastatin on bone.…”

Section: Discussionmentioning

confidence: 99%

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Design and In Vitro Evaluation of Simvastatin-Hydroxyapatite Coatings by an Electrochemical Process on Titanium Surfaces

Shifang¹,

Shi²,

He³

et al. 2014

Journal of Biomedical Nanotechnology

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The aim of this study was to deposit simvastatin-hydroxyapatite (sim-HA) coatings on titanium surfaces by an electrochemical process and evaluate osteoprogenitor cell responses to sim-HA-coated surfaces. Simvastatin was prepared onto titanium surfaces with varying concentration (10(-7), 10(-6), 10(-5) and 10(-4) mol/L). Surface characteristics were performed by FSEM, XRD and FTIR. LC-MS/MS method was used for simvastatin quantification in Sim-HA-coated surfaces. Murine calvaria-derived pre-osteoblastic cell (MC3T3-E1) proliferation, alkaline phosphatase activity (ALP) and osteocalcin release were used to measure osteoblastic activities. FSEM observation showed rod-like HA crystals covered on all surfaces. As drug concentration increased, the crystal diameter decreased. XRD and FTIR observations showed all coatings consisted of HA. LC-MS/MS test showed the simvastatin concentration in 10(-6) mol/L group was 2.77 x 10(-7) mol/L/cm2, while in 10(-7) mol/L group was 1.89 x 10(-7) mol/L/cm2. MC3T3-E1 cells grown on 10(-7) mol/L and 10(-6) mol/L Sim-HA surfaces showed increased ALP activity as compared to HA-coated surfaces on day 7 (P < 0.05) while at 10 day, all Sim-HA groups were significantly increased than HA group (P < 0.05). Two Sim-HA groups showed significant increases in osteocalcin production on 7 10 and 14 day (P < 0.05). It showed that incubation with 10(-7) mol/L simvastatin enhanced the cell proliferation to a statistically significant extent (P < 0.05) compared with the HA group at day 7. It was concluded that simvastatin was successfully deposited into HA coatings using the electrochemical process and the sim-HA coatings enhanced differentiation of osteoprogenitor cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Design and In Vitro Evaluation of Simvastatin-Hydroxyapatite Coatings by an Electrochemical Process on Titanium Surfaces

Shifang¹,

Shi²,

He³

et al. 2014

Journal of Biomedical Nanotechnology

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“…3,4 Similar results were also reported for other generic PPIs. 5,6 However, few studies have been performed to investigate the mechanisms underlying such delays.…”

Section: Introductionmentioning

confidence: 99%

Factors Contributing to Drug Release From Enteric-Coated Omeprazole Capsules: An In Vitro and In Vivo Pharmacokinetic Study and IVIVC Evaluation in Beagle Dogs

et al. 2020

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This study was performed to explore factors influencing the release of the proton pump inhibitor omeprazole from entericcoated capsules in vitro and absorption in vivo in beagle dogs. Enteric-coated pellets with different enteric coating materials and coating levels were designed and prepared. All self-prepared formulations were characterized in vitro as well as in vivo and compared to the brand and generic commercial products. Evaluation of the corresponding release profiles suggested that coating material was the most critical factor. Enteric coating level determined the lag time before initiation of drug release, and subcoating level affected the drug release rate. Pharmacokinetic studies were performed in beagle dogs to further confirm the influence of formulation factors on drug absorption. Medium at pH 6.8 was a more biorelevant condition for in vitro drug release tests, although medium at pH 6.0 was better for discriminating release profiles of different formulations. A multiple level C in vitro/in vivo correlation was preliminarily established by which T max and C max of omeprazole formulations could be predicted with release parameters such as T lag and T 25 . These results may facilitate quality evaluation and potentially improve the clinical efficacy of generic omeprazole products.

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“…[22,23,28,29] Recent LC-MS=MS and Rapid Resolution-MS=MS methods for LZ also have demerits, like higher LLOQ and longer run time (RT), complex processing, double extraction, binary solvents for extraction, and instability of the analyte of interest in the mobile phase. [12,18,26] Recently, Hishinuma et al reported estimation of LZ using lansoprazole-d4 as the IS by LC-MS=MS, which also has a longer run time of about 3.5 min. [25] Considering this, we developed a method with little sample volume, the shortest sample clean up procedure, the utilization of a simple liquid-liquid extraction using a single solvent, a new choice of IS, and the shortest run time.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography–electrospray Tandem Mass Spectrometry (Lc-MS/Ms) Determination of Lansoprazole in Human Plasma

Ganesh

Bhagiyalakshmi

Hemalatha

et al. 2011

Journal of Liquid Chromatography & Related Technologies

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& A rapid, selective, and sensitive LC-MS=MS method has been developed and validated for quantification of lansoprazole (LZ) in human plasma using esomeprazole as an internal standard (IS). The analyte and IS were extracted by single solvent liquid-liquid extraction using tert-Butyl methyl ether and separated by isocratic elution on C18 analytical column with 90:10 v=v acetonitrile, and 20 mM ammonium acetate (flow rate of 1 mL=min) as the mobile phase in the positive ion mode. Selected Reaction Monitoring transitions for LZ, internal standard and their daughter ion were observed at 369, 346, 252, and 198 m=z, respectively. The lower limit of quantification was 3.997 ng=mL and total run time for the analysis was 1.2 minutes. Linear calibration was observed in the range of 3.997 ng=mL to 2002.5 ng=mL. The method was validated for its selectivity, stability, accuracy, precision, and recovery.

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Determination of lansoprazole in human plasma by rapid resolution liquid chromatography–electrospray tandem mass spectrometry: Application to a bioequivalence study on Chinese volunteers

Cited by 18 publications

References 10 publications

Design and <I>In</I> <I>Vitro</I> Evaluation of Simvastatin-Hydroxyapatite Coatings by an Electrochemical Process on Titanium Surfaces

Design and <I>In</I> <I>Vitro</I> Evaluation of Simvastatin-Hydroxyapatite Coatings by an Electrochemical Process on Titanium Surfaces

Factors Contributing to Drug Release From Enteric-Coated Omeprazole Capsules: An In Vitro and In Vivo Pharmacokinetic Study and IVIVC Evaluation in Beagle Dogs

Liquid Chromatography–electrospray Tandem Mass Spectrometry (Lc-MS/Ms) Determination of Lansoprazole in Human Plasma

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