Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography

Rapid HPLC analysis of the antiepileptic lamotrigine and its metabolites in human plasma

“…It is a feasible procedure based on deproteinization with methanol and grants appropriate sample purification and good extraction yields with satisfactory precision. Compared to previously published pretreatment procedures based on liquid -liquid extraction [14], the pretreatment procedure presented here has demonstrated to be superior in terms of rapidity, feasibility, precision (4.3% instead of 8.1%), and extraction yield (90% instead of 80%). Moreover, the HPLC apparatus is less expensive and more readily available in clinical laboratories than other apparatus such as the HPLC-MS [15], reported in literature for the analysis of LTG together with its metabolites.…”

“…Only three papers describe the determination of LTG and its metabolites in biological fluids by means of an MEKC [18], an automated sequential trace enrichment of dialysates HPLC [19], and of an HPLC-MS [15]. The pretreatment of biological samples is usually carried out by means of deproteinization by solvents [2,13,15,16], liquid -liquid extraction [14], and SPE [17,18]. The aim of this study was the development of an easy and reliable HPLC method with diode array detection (DAD) for the simultaneous analysis of LTG and its 2-N-glucuronide and 2-N-methylated metabolites in human plasma from patients using a rapid sample pretreatment procedure.…”

Development of a dissolution test for lamotrigine in tablet form using an ultraviolet method

“…The efficacy of lamotrigine as an add-on therapy has been well established in adults and children (Ben-Menachen, 2000) and was recently approved as monotherapy (Cheng et al, 2005). Although there are many studies describing the determination of lamotrigine in biological fluids (Cocciglio et al, 1991;Sallustio, Morris, 1997;Matar et al, 1998;Ashton et al, 1999;Botinger et al, 1999;Vidal et al, 1999;Angelis Stofordis et al, 1999;Barbosa, Midio, 2000;Torra et al, 2000;Croci et al, 2001;Castel-Branco et al, 2001;Patil, Bodhankar, 2005;Cheng et al, 2005;Kuldeep, Subash, 2005), and pharmaceutical formulation (Emani et al, 2006;Yousef, Taha, 2007) by several methods besides a dissolution test using HPLC method (Sripalakit et al, 2008), no dissolution test for this pharmaceutical solid dosage form has yet been described in any pharmacopoeia. Therefore, this paper describes the development and validation of a dissolution test for lamotrigine in tablets using a simple, fast and inexpensive ultraviolet method, and performs a comparative evaluation of the dissolution profiles of two different formulations.…”

Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring

“…Many analytical methods proposed for the determination of LMT in biological fluids that include HPLC, CE, GC methods and immunoassays. HPLC separations on reversed-phase columns in isocratic mode are widely applied but normalphase columns have been also used [159][160][161]. Although the chemical structure of LMT allows the preparation of suitable hepten, the major difficulty in immunoassay development has been the high concentrations of LMT found in the clinical samples [162,163].…”