Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sources

Kilcawley, Kieran N.; Wilkinson, Martin G.; Fox, Patrick F.

doi:10.1016/s0141-0229(02)00136-9

Cited by 129 publications

(87 citation statements)

References 18 publications

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“…The activities of CAL B and Palatase preparations used in the present work were 6 U/mL and 125 U/mL respectively. The activity unit corresponded to the assay based upon hydrolysis of p-nitrophenyl palmitate [69,70]. The corresponding protein contents were found to be 8.1 mg/mL and 6.8 mg/mL respectively as established by the dye binding assay [71].…”

Section: Preparation Of Protein Coated Microcrystals (Pcmcs) Of the Ementioning

confidence: 96%

Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds

Banerjee

Singh

Solanki

et al. 2013

sustain chem process

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Background: Replacing chemical catalysts with biocatalysts is a widely recognized goal of white biotechnology. For biocatalytic processes requiring low water containing media, enzymes for example commercial preparations of lipases, show low catalytic efficiencies. Some high activity preparations for addressing this concern have been described. Protein coated microcrystals (PCMC) constitute one such preparation. The present work describes a Combi-PCMC for synthesis of biodiesel from the oil extracted from spent coffee grounds. Results: Different lipases were screened for biodiesel synthesis from crude coffee oil out of which Novozym 435 gave the best conversion of 60% in 4 h. Optimization of reaction conditions i.e. % water, temperature and purification of coffee oil further enhanced conversion upto 88% in 24 h. A mixture of Novozym 435 and a cheap commercially available 1,3-specific lipase RMIM (from Mucor miehei) was used in different ratios and 1:1 was found to be the best trade-off between conversion and cost. The commercial preparations then were replaced by a novel biocatalyst design called Combi-Protein coated microcrystals (Combi-PCMC) wherein CAL B and Palatase were co-immobilized with K 2 SO 4 as the core and this performed equivalent to the commercial preparations giving 83% conversion in 48 h.

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Section: Preparation Of Protein Coated Microcrystals (Pcmcs) Of the Ementioning

confidence: 96%

Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds

Banerjee

Singh

Solanki

et al. 2013

sustain chem process

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“…Flavourzyme® (EC: 3.4.11.1) is a fungal complex of exopeptidases and endoproteases used for cheese production and acceleration of cheese ripening, especially for debittering and flavor development of cheese (Fox 1993;Fox and Grufferty 1991;Kilcawley et al 2002). Commercially, this enzyme is in a powder form obtained from Aspergillusoryzae.…”

Section: Introductionmentioning

confidence: 99%

“…Commercially, this enzyme is in a powder form obtained from Aspergillusoryzae. Kilcawley et al (2002) showed that Flavourzyme has an endo-and amino-peptidase activity. The potential advantages of liposome encapsulation of enzyme for cheese acceleration includes usage of natural ingredients, protection of casein from early proteolysis and well partition of enzyme in the curd (El Soda 1993;Mozafari et al 2008a, b).…”

Section: Introductionmentioning

confidence: 99%

The encapsulation of flavourzyme in nanoliposome by heating method

et al. 2013

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The main objective of this study was to use heating method (HM) to prepare liposome without employing any chemical solvent or detergent. Plackett-Burman design (PBD) was applied for the screening of significant process variables including the lecithin proportion, the cholesterol/ lecithin ratio, the pH of solution for liposome preparation, the enzyme/lecithin ratio, the stirring time, the process temperature, the speed of stirrer, the ratio of stirrer to the tank diameter, the application of homogenization, the method of adding enzyme and centrifugation conditions on the encapsulation efficiency (EE %) of liposome and the activity of liposomal Flavourzyme (LAPU −1 ) (P<0.05). Then, the response surface methodology based on the central composite design (CCD) was applied for the evaluation of the impacts of the significant mentioned variables on the EE (%) and the activity of the liposomal Flavourzyme. The results indicated that the lecithin proportion and the stirring time were the major influential variables for both responses. The most suitable formulation of the Flavourzyme-loaded liposome is 4.5 % lecithin, 45°C temperature, 5 % Flavourzyme/lecithin ratio, 30 min stirring time and medium pH of 6. Under suitable operating conditions, the EE of liposome and the activity of the liposomal Flavourzyme were achieved as 26.5 % and 9.96 LAPU ml −1 , respectively. AFM technique and size distribution clearly showed the diameter of 189 nm for the spherical shape of the Flavourzyme-loaded nanoliposome.

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“…The latter type, such as proline-specific aminopeptidase, removes certain N-terminal amino acid residues from peptides specifically. 1,2 APs are associated with many human diseases and play an important role in a wide range of biological processes. [3][4][5][6] The research to elucidate the catalytic mechanisms of APs is significant for medicine and pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Structure-based approach to alter the substrate specificity ofBacillus subtilisaminopeptidase

Gao¹,

Cui²,

Ding

et al. 2013

Prion

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Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sources

Cited by 129 publications

References 18 publications

Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds

Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds

The encapsulation of flavourzyme in nanoliposome by heating method

Structure-based approach to alter the substrate specificity ofBacillus subtilisaminopeptidase

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