Determination of kanamycin in serum by solid-phase extraction, pre-capillary derivatization and capillary electrophoresis

Long, Yinhua; Hernández, Margarita; Kaale, Eliangiringa; Schepdael, Ann Van; Roets, Eugène; Borrull, Francesc; Calull, Marta; Hoogmartens, Jos

doi:10.1016/s1570-0232(02)00804-8

Cited by 72 publications

(39 citation statements)

References 16 publications

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“…Therefore, careful monitoring of the drug level in the serum of patients receiving kanamycin is required, especially in long-term therapy. The existing methods for the determination of kanamycin in blood samples include microbiological methods [71] and immunoassay [72]. These methods are nonspecific and the results are affected by the presence of other aminoglycoside antibiotics.…”

Section: Kanamycinmentioning

confidence: 99%

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Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

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This review is a continuation of the previous reviews (Electrophoresis 1999, 20, 3259-3268; Electrophoresis 2001, 22, 4244-4248) to update the recent publications from 2001 to 2003 on pharmacokinetic studies using capillary electrophoresis (CE). During this period of time, CE remains as a unique analytical method for some studies, which would otherwise be limited by many factors, such as the sample volume, detection sensitivity, or separation power. It is particularly noticeable that the separation of chiral drugs in biological sample and the use of solid-phase extraction (SPE) as a simple and convenient means of sample preparation appear to become popular for CE-based assays. The use of CE for assessing complete pharmacokinetic information, however, did not show a significant growth during this period of time. In order to provide a broad range of view on how biological samples are analyzed by CE, this review will cover publications during the past two years on the use of CE for the analysis of drugs in biological fluids for general pharmacokinetic applications including drug monitoring and bioavailability studies.

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Section: Kanamycinmentioning

confidence: 99%

“…However, ECD in CE requires a special setup and technique. Van Schepdael et al [71] reported an effective CE method based on UV detection for the determination of kanamycin in human serum. Off-line SPE was employed for the preparation of kanamycin from serum using a carboxypropyl-bonded phase weak-cation-exchange cartridge.…”

Section: Kanamycinmentioning

confidence: 99%

Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

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“…However, during the analysis of six different commercial samples by CE and LC, unexplainable differences in the levels of kanamycin A were found between the two methods [2]. This system was applied to the determination of kanamycin in serum [3].…”

Section: Aminoglycosidesmentioning

confidence: 99%

“…A commercial eye drop sample containing prednisolone acetate, sulfacetamide sulfate and phenylephrine was diluted with 30% MeOH. The analytes were separated in a 5 mM phosphate-5 mM borate buffer, pH 8.2, containing 50 mM SDS and 3% v/v CH 3 …”

Section: Eye Dropsmentioning

confidence: 99%

Analysis of antibiotics by capillary electrophoresis

Flurer

2003

Electrophoresis

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Recent developments in the characterization of antibiotics are reviewed. Many capillary electrophoretic techniques have been utilized in their analyses, addressing various aspects of quantifying, profiling, and monitoring. Laser-induced fluorescence detection systems demonstrated their usefulness in clinical settings and in the monitoring of residue levels in food matrices. Different sample introduction methods have been explored, enhancing detection sensitivity, or reducing or eliminating sample manipulation prior to injection.

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“…Till date, KS has been formulated as transdermal patch (Lopez-Cervantes et al, 2009), Freeze dried microparticles Analysis of KS by CE in drug substances and serum has also been attempted (Kaale et al, 2001;Kaale et al, 2003;Holzgrabe et al, 2008), but selectivity and precision are rather poor compared to LC. Detection systems based on conductivity (Claes et al, 1973), colorimetry after derivatization with ninhydrin (Inouye et al, 1964), UV spectrophotometry after precolumn or precapillary derivatization (Longa et al, 2003;Gambardella et al, 1985;Kaale et al, 2001),and UV detection after in capillary derivatization have been described (Kaale et al, 2003;Holzgrabe et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Assay for the Analysis of Kanamycin sulphate nanoparticles in Rat after intramuscular administration: Application to a Pharmacokinetic Study

Mustafa¹,

Devi²

2016

J App Pharm Sci

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A rapid reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of Kanamycin sulphate (KS) in PLGA nanoparticle formulation. A new formulation of KS loaded PLGA nanoparticles (NPs) was prepared by double (multiple) emulsion process in our laboratory. The desired chromatographic separation was achieved on a Phenomenex C18column under isocratic conditions using UV detection at 205 nm. The optimized mobile phase consisted of a mixture of 0.1 M disodium tetraborate (pH 9.0) and water (25:75, v/v) supplemented with 0.5 g/L sodium octanesulphonate at a flow rate of 1 mL/min. The linear regression analysis for the calibration curves showed a good linear correlation over the concentration range of 120-840µg/ml, with correlation coefficients of (r 2 0.9997). The system was found to construct sharp peaks for KS and IS with retention times of 4.08 and 5.49 min, respectively. Transmission electron microscopy studies on MFX NPs demonstrated particle size < 100 nm. An average encapsulation efficiency of 74.34% was obtained for NPs. In vitro studies showed zero-order release and about 95% drug being released within 12 days in PBS (pH 7.4). In conclusion, the proposed optimized method was successfully applied for the determination of in vitro and in vivo release studies of KS NPs.

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Determination of kanamycin in serum by solid-phase extraction, pre-capillary derivatization and capillary electrophoresis

Cited by 72 publications

References 16 publications

Recent progress in pharmacokinetic applications of capillary electrophoresis

Recent progress in pharmacokinetic applications of capillary electrophoresis

Analysis of antibiotics by capillary electrophoresis

Liquid Chromatographic Assay for the Analysis of Kanamycin sulphate nanoparticles in Rat after intramuscular administration: Application to a Pharmacokinetic Study

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