Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

Klejdus, Bořivoj; Vacek, Jan; Adam, Vojtěch; Zehnálek, Josef; Kizek, René; Trnková, Libuše; Kubáň, Vlastimil

doi:10.1016/j.jchromb.2004.03.044

Cited by 79 publications

(45 citation statements)

References 53 publications

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“…As result of the importance of DZ in biological systems, different methods have been applied for the determination of DZ in foods and biological fluids including gas chromatography (GC) [11], GC-MS [12] high-performance liquid chromatography (HPLC) [13][14][15], liquid chromatography (LC) [16], capillary zone electrophoresis (CZE) with UV detection [17], electrochemical detection [18] capillary electrophoresis with electrochemical detection [19], and hydrodynamic and cyclic voltammetry [4,5,[18][19][20][21]. However, more recently, there has been a tendency for the use of less costly techniques and a reduction in the use of organic solvents since they result in high ecological costs.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Oxidation Mechanisms of the Antioxidants Daidzein and 7‐Hydroxy‐4‐chromone

et al. 2012

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The electrochemical behavior of antioxidants daidzein (DZ) and 7-hydroxy-4-chromone (7-OH-4-CHM) was investigated. The oxidation of DZ is irreversible, pH-dependent, and occurs in two steps, the first of 4'-OH on the B-ring and the second on the 7-OH on the A-ring. The oxidation of 7-OH-4-CHM occurs on the 7-OH on the A-ring, is irreversible, pH-dependent and proceeds in a single step. The DZ diffusion coefficient was calculated in pH 7.0 phosphate buffer to be D DZ = 8.1 10 À5 cm 2 s À1. The detection limits of DZ and 7-OH-4-CHM was calculated as LOD DZ = 0.08 mM and LOD 7-OH-4-CHM = 0.13 mM.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Oxidation Mechanisms of the Antioxidants Daidzein and 7‐Hydroxy‐4‐chromone

et al. 2012

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“…As it was reported, presence of organic solvent in mobile phase can markedly influence both current response and separation of the target molecules [42,43,49,50]. HPLC-ED chromatograms of the twelve thiols measured in the presence of mobile phase consisted of 80 mM TFA and 0, 3 or 5% methanol are shown in Figure 5.…”

Section: Coulometric Detection -Coularray Detectormentioning

confidence: 73%

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

et al. 2010

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The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

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“…When analytes covering a wide range of polarities have to be extracted, mixtures of low-and high-polar solvents generally provide better results than single solvents. Alternatively, two extractions-one with a non-polar, and the second one with a more polar solvent-can be applied [10,11].…”

Section: Sample Preparation Methods Pressurized Liquid and Subcriticamentioning

confidence: 99%

Modern Methods of Sample Preparation for GC Analysis

Koning¹,

Janssen

Brinkman

2009

Chroma

122

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Today, a wide variety of techniques is available for the preparation of (semi-) solid, liquid and gaseous samples, prior to their instrumental analysis by means of capillary gas chromatography (GC) or, increasingly, comprehensive two-dimensional GC (GC 9 GC). In the past two decades, a large number of 'modern' sample-preparation techniques has been introduced, which have partly superseded their 'classical' counterparts. These novel techniques include off-line and on-line (sometimes semi-or fully automated) procedures, and exhaustive extraction as well as equilibrium techniques. In order to improve overall performance, aspects such as essentially organic solvent-less approaches, large-volume injection and miniaturization receive increasing attention. In most recent applications, mass spectrometric or element-selective detection have been used. The present review discusses the advantages and disadvantages, and relative performance, of most of the modern samplepreparation techniques and cites a number of illustrative applications for each of them.

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Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

Cited by 79 publications

References 53 publications

Electrochemical Oxidation Mechanisms of the Antioxidants Daidzein and 7‐Hydroxy‐4‐chromone

Electrochemical Oxidation Mechanisms of the Antioxidants Daidzein and 7‐Hydroxy‐4‐chromone

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Modern Methods of Sample Preparation for GC Analysis

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