Determination of interstitial collagenase (MMP-1) in patients with rheumatoid arthritis.

Maeda, Shinsaku; Sawai, Takashi; Uzuki, Miwa; Takahashi, Yuichi; Omoto, Hiroshi; Seki, Masaaki; Sakurai, Masaki

doi:10.1136/ard.54.12.970

Cited by 63 publications

(49 citation statements)

References 40 publications

(11 reference statements)

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“…2. The degree of synovial inflammation was analysed on hematoxylin and eosin stained sections (32), and determined as inflammation (INF) scores based on the sum of three components; synovial lining thickness (0 ϭ 1-2 cells; 1ϩ ϭ 3-4 cells; 2ϩ ϭ 5-6 cells; 3ϩ ϭ Ͼ6 cells), lymphoid follicles (0 ϭ 0-1 per low power field (lpf)(ϫ40); 1ϩ ϭ 2-3/lpf; 2ϩ ϭ 4-6/lpf; 3ϩ ϭ Ͼ6/lpf), and interfollicular features (0 ϭ complete fibrosis; 1ϩ ϭ mononuclear cell infiltration Ͻ33% of lpf; 2ϩ ϭ 33-67%; 3ϩ ϭ Ͼ67%).…”

Section: Discussionmentioning

confidence: 99%

Human parvovirus B19 as a causative agent for rheumatoid arthritis

Takahashi

Murai

Shibata

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

248

142

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Section: Discussionmentioning

confidence: 99%

Human parvovirus B19 as a causative agent for rheumatoid arthritis

Takahashi

Murai

Shibata

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

248

142

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“…In rheumatoid synovial tissue, increased amounts of MMPs are expressed. [5][6][7][8][9][10] Levels of MMP in the synovium and serum correlate with disease activity 11 and radiographic damage, 12,13 suggesting that MMPs play a role in the destructive process in RA. Therefore, inhibition of MMPs may be a therapeutic strategy to prevent joint destruction in RA, and the delivery of naturally occurring inhibitors of MMPs may serve as one option for achieving this goal.…”

Section: Introductionmentioning

confidence: 99%

Cartilage degradation and invasion by rheumatoid synovial fibroblasts is inhibited by gene transfer of TIMP-1 and TIMP-3

Laan

Quax

Seemayer³

et al. 2003

Gene Ther

103

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Matrix metalloproteinases (MMPs) are believed to be pivotal enzymes in the invasion of articular cartilage by synovial tissue in rheumatoid arthritis (RA). Here, we investigated the effects of gene transfer of tissue inhibitors of metalloproteinases (TIMPs) on the invasiveness of RA synovial fibroblasts (RASF) in vitro and in vivo. Adenoviral vectors (Ad) were used for gene transfer. The effects of AdTIMP-1 and AdTIMP-3 gene transfer on matrix invasion were investigated in vitro in a transwell system. Cartilage invasion in vivo was studied in the SCID mouse coimplantation model for 60 days. In addition, the effects of AdTIMP-1 and AdTIMP-3 on cell proliferation were investigated. A significant reduction in invasiveness was demonstrated in vitro as well as in vivo in both the AdTIMP-1-and AdTIMP-3-transduced RASF compared with untransduced SF or SF that were transduced with control vectors. In vitro, the number of invading cells was reduced to 25% (Po0.001) in the AdTIMP-1-transduced cells and to 13% (Po0.0001) in the AdTIMP-3-transduced cells (% of untransduced cells). Cell proliferation was significantly inhibited by AdTIMP-3 and, less, by AdTIMP-1. In conclusion, overexpression of TIMP-1 and TIMP-3 by Ad gene transfer results in a marked reduction of the invasiveness of RASF in vitro and in the SCID mouse model. Apart from the inhibition of MMPs, a reduction in proliferation rate may contribute to this effect. These results suggest that overexpression of TIMPs, particularly TIMP-3 at the invasive front of pannus tissue, may provide a novel therapeutic strategy for inhibiting joint destruction in RA.

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“…Collagenases have been detected in the synovial fluid of patients with traumatic arthritis, RA, and OA (16)(17)(18)(19). In the synovial joint, there are three distinct tissue sources of the collagenases that may be involved in the degradation of articular cartilage.…”

Section: Introductionmentioning

confidence: 99%

Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage.

Billinghurst¹,

Dahlberg²,

Ionescu³

et al. 1997

J. Clin. Invest.

906

837

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We demonstrate the direct involvement of increased collagenase activity in the cleavage of type II collagen in osteoarthritic human femoral condylar cartilage by developing and using antibodies reactive to carboxy-terminal (COL2-3/ 4C short ) and amino-terminal (COL2-1/4N1) neoepitopes generated by cleavage of native human type II collagen by collagenase matrix metalloproteinase (MMP)-1 (collagenase-1), MMP-8 (collagenase-2), and MMP-13 (collagenase-3). A secondary cleavage followed the initial cleavage produced by these recombinant collagenases. This generated neoepitope COL2-1/4N2. There was significantly more COL2-3/ 4C short neoepitope in osteoarthritis (OA) compared to adult nonarthritic cartilages as determined by immunoassay of cartilage extracts. A synthetic preferential inhibitor of MMP-13 significantly reduced the unstimulated release in culture of neoepitope COL2-3/4C short from human osteoarthritic cartilage explants. These data suggest that collagenase(s) produced by chondrocytes is (are) involved in the cleavage and denaturation of type II collagen in articular cartilage, that this is increased in OA, and that MMP-13 may play a significant role in this process. ( J. Clin. Invest. 1997. 99:1534-1545.)

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Determination of interstitial collagenase (MMP-1) in patients with rheumatoid arthritis.

Cited by 63 publications

References 40 publications

Human parvovirus B19 as a causative agent for rheumatoid arthritis

Human parvovirus B19 as a causative agent for rheumatoid arthritis

Cartilage degradation and invasion by rheumatoid synovial fibroblasts is inhibited by gene transfer of TIMP-1 and TIMP-3

Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage.

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