DETERMINATION OF in vitro BIOFILM FORMATION ABILITIES OF FOOD BORNE Salmonella enterica ISOLATES

Abstract

Salmonellosis caused by non-typhodial Salmonella enterica serotypes is one of the most important food-borne diseases worldwide and biofilm structure formed by these pathogens provide a reservoir for food contamination and a source for infections. This study was performed in order to determine biofilm formation abilities of food borne Salmonella isolates on polystyrene and on air liquid interphase and their colony morphologies when grown on Congo Red Agar plates. 32 food-borne Salmonella strains isolated from retail chicken carcasses in Edirne province of Turkey and belonging to the Infantis, Enteritidis, Kentucky and Telaviv serotypes were used. The microtiter plate technique was used to determine biofilm formation abilities of the isolates on polystyrene surfaces by measuring the optical density (OD) values of the stained bacterial biofilms. The results showed that the strongest biofilm formation capacities of the isolates were observed at 22°C for 3 days of incubation. Although all isolates formed pellicle on the liquid-air interface at 22°C, only 13% of the isolates belonging to the Infantis, Kentucky and Enteritidis serovars formed pellicle at liquid-air interface at 37°C. Three different colony morphotypes (saw; smooth and white, bdar; brown, dry and rough, rdar; red, dry and rough) were determined on Congo Red Agar among the isolates. High biofilm formation abilities of the tested Salmonella isolates can lead to widespread of virulence and resistance properties, especially to medically important antibiotics such as ciprofloxacin, via food chain. This situation constitutes an important concern for public health.

Disinfectant resistance of <i>Salmonella</i> in <i>in vitro</i> contaminated poultry house models and investigation of efficient disinfection methods using these models

“…1 ). Salmonella strains may change the quantity of biofilms depending on their culture conditions [ 2 , 16 ]; however, the different levels of biofilm formation among our clonal strains were observed under the same culture conditions. Therefore, the intrinsic ability or optimum conditions for biofilm formation may easily change in Salmonella in the field.…”

Understanding Antimicrobial Resistance (AMR) Profiles of Salmonella Biofilm and Planktonic Bacteria Challenged with Disinfectants Commonly Used During Poultry Processing

“…It is well known that there is a correlation between Salmonella biofilm formation and persistence in factory environments; however, several studies have shown that different serovars of Samonella have different capacities to make biofilm under different environmental conditions [80,81,82]. For example, when studying Salmonella Enteritidis, Infantis, Kentucky and Telaviv serotypes at different temperatures, a shift in biofilm formation capacities was observed with most of the serotypes becoming strong biofilm producers at 22 °C [82]. In contrast, at 37 °C, only some of the Salmonella Enteritidis and Infantis serovars were considered strong biofilm producers.…”