Determination of Illicit Drugs in Dried Blood Spots by LC–MS/MS Method: Validation and Application to Real Samples

Detection of 26 Drugs of Abuse and Metabolites in Quantitative Dried Blood Spots by Liquid Chromatography–Mass Spectrometry

“…To date, many analytical protocols have been developed to determine/quantify drugs of abuse in DBSs by modern analytical techniques, including liquid chromatography tandem–mass spectrometry (LC–MS/MS) [ 16 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ] mainly for quantification purposes; liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) [ 17 , 27 ] and gas chromatography–mass spectrometry (GC–MS) [ 11 , 28 ] for drug screening. Nowadays, various methodologies have been developed for DBSs for both quantification and screening purposes, offering precise analyte level measurements and efficiency of detection in biological samples.…”

Recent advances in the detection of drugs of abuse by dried blood spots

“…Owing to the technological advances over the last decade, LC–MS/MS instruments currently achieve the highest levels of sensitivity and specificity in targeted drug analysis. Extreme sensitivity is obtained by triple quadrupole detectors operating in the multiple reaction monitoring mode, while the newest TOF (time‐of‐flight) and Orbitrap analyzers additionally allow high‐resolution mass analysis and accurate determination of elemental composition, resulting in accurate qualitative and quantitative analysis of substances present in blood even at extremely low concentrations (Antelo‐Domínguez et al, 2013; Joye et al, 2019; Kacargil et al, 2020; Keevil, 2011; Li & Tse, 2010; Sadler Simões et al, 2018; Stove et al, 2012). The present review provides an overview of the most recent studies that propose easy and highly sensitive procedures for the detection of drugs of abuse after DBS sampling.…”

“…The analysis of target analytes after DBS collection may be performed by several analytical techniques, according to the application: Kacargil et al, 2020;Keevil, 2011;Li & Tse, 2010;Sadler Simões et al, 2018;Stove et al, 2012). The present review provides an overview of the most recent studies that propose easy and highly sensitive procedures for the detection of drugs of abuse after DBS sampling.…”

“…In a similar context,Kacargil et al (2020) presented the development, validation and application of real samples in a DBS method based on LC-MS/MS and aimed at the detection of illicit drugs. This approach used higher volumes of spotted blood (100 μl) and extraction solvent (4 ml), and involved higher extraction time and longer instrumental analysis than those previously cited.…”

Dried Spots associado com um método LC-DAD para determinação simultânea de MDMA (ecstasy) e MDA em amostras de fluído oral

“…Assim, selecionouse 10 min como sendo o tempo de sonicação. SAUSSEREAU et al, 2012), mas solventes menos polares como acetonitrila e acetato de etila também são utilizados (JANTOS et al, 2011;STOYKOVA et al, 2016;KACARGIL;DAGLIOGLU;GOREN, 2020). O tempo de amostragem do DOFS antes da injeção no sistema cromatográfico foi de 4 h, que está dentre o período observado nos trabalhos (entre 1,0 e 16 h) disponíveis na literatura.…”