Determination of hydroxyproline in rat urine by high-performance liquid chromatography with electrogenerated chemiluminescence detection using tris(2,2′-bipyridyl) ruthenium(II)

Ikehara, Tatsuya; Habu, Naoko; Nishino, Ikuko; Kamimori, Hiroshi

doi:10.1016/j.aca.2004.12.060

Cited by 19 publications

(8 citation statements)

References 24 publications

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“…Total urinary hydroxyproline (Hyp) has been determined by means of HPLC-ECL using Ru(bpy) 3 2+ [73]. After sample treatment, Hyp was separated by column-switching HPLC and ECL detection, with recoveries in urine samples higher than 96%.…”

Section: Determination Of Amino Acidsmentioning

confidence: 99%

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

Gámiz‐Gracia

Garcı́a-Campaña

Huertas-Pérez

et al. 2009

Analytica Chimica Acta

161

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Section: Determination Of Amino Acidsmentioning

confidence: 99%

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

Gámiz‐Gracia

Garcı́a-Campaña

Huertas-Pérez

et al. 2009

Analytica Chimica Acta

161

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“…Hyp, which is absent in other proteins , is an amino acid unique to collagen and provides an important marker to directly quantify the hydrolyzed protein . Many analytical methods have been developed to determine Hyp in different samples, such as spectrophotometry , CE with electrochemiluminescene detection , anion‐exchange chromatography , GC , HPLC , LC–MS , and ELISA . Although spectrophotometry and HPLC methods are the most widely used techniques for determination of Hyp, these methods lack sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Determination of l‐hydroxyproline using hydrophilic interaction chromatography coupled to tandem mass spectrometry with lyophilized concentrated extraction in milk and dairy products

Zhu

Peng

Chen

et al. 2014

J of Separation Science

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In this study, a screening and confirmation method for the determination of l-hydroxyproline (Hyp) as a target compound in milk and dairy products using high-performance liquid chromatography with tandem mass spectrometry was developed. The samples were lyophilized after acidic hydrolysis, followed by cleanup with graphitized carbon black to remove pigments. Hyp was separated by a hydrophilic interaction chromatographic column and analyzed by high-performance liquid chromatography with tandem mass spectrometry working with multiple reaction monitoring mode using an electrospray ionization interface in a positive-ion mode. Average recoveries in spiked milk and dairy products ranged from 68.0 to 101.1% with relative standard deviations between 2.0 and 11.7% (n = 7). A reagent-matched standard calibration curve was used for quantification of Hyp, with linear correlation coefficient (R(2)) > 0.99 in the concentration range of 0.1-100 μg/mL. The LOQs were from 0.25 to 5 mg/kg, which were usually sufficient to verify the Hyp in samples. The confirmation concentration of Hyp ranged from 10 to 50 mg/kg.

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“…The column-switching system avoids off-line sample preparation procedures such as traditional solid-phase extraction or liquid-liquid extraction. [15][16][17][18][19] This technique allows plasma samples to be directly injected onto the HPLC system, thereby resulting in an accurate assay without internal standards. As shown in Chart 1, detection using tris(2,2-bipyridine) ruthenium(III) (Ru(bpy) 3 3+ ) has been shown to be sensitive and selective for reducing agents such as indoles, 20) oxalate, 21) active methylenes, 22) and tertiary amines.…”

mentioning

confidence: 99%

Determination of Miglitol by Column-Switching Ion-Pair HPLC with Tris(2,2′-bipyridine)ruthenium(II)-Electrogenerated Chemiluminescence Detection

Asamoto

Nobushi

et al. 2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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We have developed a highly sensitive, simple method for the quantitative determination of miglitol in standard serum samples using column-switching ion-pair HPLC with tris(2,2′-bipyridine)ruthenium(II)-electrogenerated chemiluminescence detection. The serum samples were directly injected into a column-switching HPLC system with a Shim-pack MAYI-SCX precolumn to remove the serum matrix. Chromatographic separation of miglitol was achieved on a TSKgel ODS 100-V column using a mobile phase containing sodium 1-octanesulfonate as an ion-pair reagent. The detection and quantification limits of miglitol were 3 and 10 ng/ mL, respectively. The calibration curve for miglitol in the serum samples showed good linearity (r 2 0.9997) in the range of 10-2500 ng/mL. The recovery rate of miglitol from the serum samples was more than 94% as calculated from blank serum samples spiked with miglitol 50, 100, 500, 1000, and 2000 ng/mL. Therefore, this method can be applied to routine therapeutic monitoring of miglitol in serum samples.Key words miglitol; tris(2,2′-bipyridine)ruthenium(II); column-switching ion-pair HPLC Type 2 diabetes, formerly called non-insulin-dependent diabetes, is a costly disease; in 2013, approximately 300 million people worldwide were affected, according to the International Diabetes Federation.1) Miglitol, a type of α-glucosidase inhibitor (α-GI), is useful for the treatment of type 2 diabetes because it inhibits the activity of disaccharide-hydrolyzing enzyme in the small intestine, which has a lowering effect on postprandial blood glucose and insulin levels. [2][3][4][5] Recently, it was demonstrated that miglitol prevents reactive hypoglycemia secondary to late dumping syndrome. Furthermore, miglitol is more effective than two other α-GIs, voglibose and acarbose.6) To achieve rapid therapeutic monitoring and pharmacokinetic studies of miglitol, a rapid and simple method for its quantification in blood samples is required. The enzyme inhibition assay for quantification of miglitol in plasma and urine samples seems unreliable with regard to sensitivity and selectivity.7) An alternative is the liquid chromatographic-tandem mass spectrometry (LC-MS/MS) assay for the quantification of miglitol in plasma, 8,9) which is sensitive and can give reliable results, but requires expensive instrumentation and has a high operating cost. In addition, this method requires a time-consuming cleanup procedure to remove interference in biological samples. Thus, it is not suitable for routine analysis of miglitol.We previously reported the development and application of a column-switching HPLC method with tris(2,2′-bipyridine)-ruthenium(II)-electrogenerated chemiluminescence (ECL) detection. [10][11][12][13][14] Chemiluminescence (CL) has become a powerful analytical tool for a large variety of analyses because it has a low detection limit and wide linear dynamic range while requiring relatively simple instrumentation. The column-switching system avoids off-line sample preparation procedures such as traditional solid-phase ex...

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Determination of hydroxyproline in rat urine by high-performance liquid chromatography with electrogenerated chemiluminescence detection using tris(2,2′-bipyridyl) ruthenium(II)

Cited by 19 publications

References 24 publications

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

Determination of l‐hydroxyproline using hydrophilic interaction chromatography coupled to tandem mass spectrometry with lyophilized concentrated extraction in milk and dairy products

Determination of Miglitol by Column-Switching Ion-Pair HPLC with Tris(2,2′-bipyridine)ruthenium(II)-Electrogenerated Chemiluminescence Detection

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