Determination of Hydrogen Peroxide Concentrations By Flow Injection Analysis Based On the Enhanced Chemiluminescent Reaction Using Peroxidase

Еремин, С. А.; Vlasenko, S. B.; Осипов, А. И.; Еремина, И. Д.; Egerov, A. M.

doi:10.1080/00032718908051233

Cited by 16 publications

(6 citation statements)

References 7 publications

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“…Previously reported methods (14) for assaying H 2 O 2 by FI-CL did not combine the mixing of chemiluminescent reagents and the detection of light emission; instead, a mixing coil was used to mix specimens and the chemiluminescent reagents outside a chemiluminometer (6,9,10). Our micro-flow injection-chemiluminescence method combines the mixing and measuring cell and is the basis of a highly sensitive detection method for H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…H 2 O 2 in biological specimens is usually present in very low concentrations and is unstable. Flow injection-chemiluminescence methods (4) have been employed recently for a highly sensitive and rapid assay of H 2 O 2 (10 À6 À 10 À4 mol/L in biological specimens) and the horseradish peroxide (HRP)-catalysed luminol chemiluminescence (5) reaction has been used as the detection method (2,(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

“…Slow delivery (under 0.1 mL/min) by FI-CL system, however, is expected to increase sensitivity of CL detection. The reason for the conventional relatively rapid flow rates is that there is no effective mixing device for chemiluminescent reagents (6) and the use of a peristaltic pump.…”

Section: Introductionmentioning

confidence: 99%

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Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Nozaki

Kawamoto

2000

Luminescence

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A novel flow cell reactor was developed for micro-flow injection determination of hydrogen peroxide (H(2)O(2)) using horseradish peroxide (HRP)-catalysed luminol chemiluminescence. The newly developed flow cell reactor for a chemiluminometer allowed mixing of the chemiluminescent reagents in front of a photomultiplier for maximum detection of the emitted light. The rapid mixing allowed a decrease in the flow rate of the pump to 0.1-0.01 mL/min, resulting in increased sensitivity of detection of light. The flow cell reactor was made by packing HRP-immobilized gels into a flow cell (Teflon tube; 6 cm x 0.98 mm i.d.) located in the cell holder of a chemiluminometer (flow-through type). The HRP-immobilized gels were made by immobilizing HRP onto the Chitopearl gel by the periodate method. H(2)O(2) specimens (50 microL) were injected into a stream of water delivered at a flow rate of 0.1 mL/min and mixed with a luminol solution (0.56 mmol/L in Tricine buffer, pH 9.2) delivered at 0.1 mL/min in the flow cell reactor. Within-run reproducibility of the assay of H(2)O(2) was 2.4% (4.85 micromol/L; flow rate 0.1 mL/min, injection interval 10 min). The reproducibility of the H(2)O(2) assay was influenced by the flow rates and the injection intervals of the H(2)O(2) specimens. As the flow rates decreased, both the light intensity and the light duration increased. Optimal light intensity was obtained at a luminol concentration of 3-8 mmol/L, but 0.56 mmol/L was sufficient for assay of H(2)O(2) in clinical specimens. At a luminol concentration of 0.56 mmol/L, the regression equation of the standard curve for H(2)O(2) (0-9.7 micromol/L) was Y = 27.5 X(2) + 394 X + 58.9 (Y = light intensity; X = concentration of H(2)O(2)) and the detection limit of H(2)O(2) was 0.2 micromol/L. This method was used to assay glucose (2.7-16.7 mmol/L) based on a glucose oxidase (20 U/mL, pH 7.4) reaction. The standard curve for glucose was Y = 167 X(2) - 351 X + 1484 (Y = light intensity; X = glucose). The within-run reproducibility for an aqueous glucose standard (2.7 mmol/L) and a control serum (glucose, 5 mmol/L) was 4.48% (n = 5) and 5.70% (n = 9), respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Nozaki

Kawamoto

2000

Luminescence

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“…Using this reaction system, Nieman [19] determined hydrogen peroxide in synthetic water with a reported detection limit of 1 lM. Eremin et al [20] and Olssen [21] used the same CL reaction system, with peroxidase and microperoxidase as catalysts, and determined the contents of hydrogen peroxide in synthetic samples with detection limits of 1 lM and 3 nM, respectively. Nabi and Worsfold [18] reported a detection limit of hydrogen peroxide at femtomole level, while others [22,23], have successfully determined the ambient levels of hydrogen peroxide in sea water in the range of 5-500 nM using cobalt(II) as catalyst.…”

Section: Introductionmentioning

confidence: 99%

Determination of hydrogen peroxide in natural waters by stopped-flow injection analysis with chemiluminescence detection

Fan

Hart

McKelvie

2000

Lab. Robotics Autom.

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“…The use of enhanced chemiluminescence for detection of minimal concentrations of H202 has previously been demonstrated (9). Modifying a commercially available test kit combination, we have developed a sensitive quantitative assay, based on peroxidase-dependent luminol-enhanced chemiluminescence, for H 2 0 2 determination in supernatants of adherent cell monolayer cultures.…”

Section: Intro Du Ctlq Nmentioning

confidence: 99%

Measurement of endogenous and TNFα‐mediated H₂O₂ production in supernatants of SK‐N‐SH neuroblastoma cells with an enhanced chemiluminescence assay

Carmine

Bruchelt

Hahn

et al. 1994

J. Biolumin. Chemilumin.

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A sensitive peroxidase-dependent luminol-enhanced chemiluminescence (ECL) assay for determination of hydrogen peroxide (H2O2) generation by tumour cells was established. This test system allows determination of H2O2 in concentrations as low as 25 pmol (50 nmol/L) and yields results which are comparable to those obtained using a less sensitive photometric method and a previously described scopoletin fluorescence assay. After 3 h incubation time 10(4) SK-N-SH neuroblastoma cells released 60 +/- 5 pmol H2O2 in the supernatant and this level was significantly (p < 0.025) increased by about 70% in the presence of 5 pmol (100 ng/mL) recombinant tumour necrosis factor alpha (TNF alpha). In contrast, H2O2 production was slightly reduced by TNF alpha at a very low concentration of 0.5 fmol (0.01 ng/mL).

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Determination of Hydrogen Peroxide Concentrations By Flow Injection Analysis Based On the Enhanced Chemiluminescent Reaction Using Peroxidase

Cited by 16 publications

References 7 publications

Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Determination of hydrogen peroxide in natural waters by stopped-flow injection analysis with chemiluminescence detection

Measurement of endogenous and TNFα‐mediated H₂O₂ production in supernatants of SK‐N‐SH neuroblastoma cells with an enhanced chemiluminescence assay

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Determination of Hydrogen Peroxide Concentrations By Flow Injection Analysis Based On the Enhanced Chemiluminescent Reaction Using Peroxidase

Cited by 16 publications

References 7 publications

Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Determination of hydrogen peroxide by micro-flow injection-chemiluminescence using a coupled flow cell reactor chemiluminometer

Determination of hydrogen peroxide in natural waters by stopped-flow injection analysis with chemiluminescence detection

Measurement of endogenous and TNFα‐mediated H2O2 production in supernatants of SK‐N‐SH neuroblastoma cells with an enhanced chemiluminescence assay

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Measurement of endogenous and TNFα‐mediated H₂O₂ production in supernatants of SK‐N‐SH neuroblastoma cells with an enhanced chemiluminescence assay