Determination of Hormones Residues in Milk by Gas Chromatography-Mass Spectrometry

Matraszek-Żuchowska, Iwona; Woźniak, Barbara; Posyniak, Andrzej

doi:10.1007/s12161-016-0620-5

Cited by 22 publications

(6 citation statements)

References 22 publications

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“…The composition of milk most often leads to difficulties in the analytical determination of antimicrobials. The high content of carbohydrates/lactose, fat and protein can often interfere with the analytical method ( 18 , 21 , 24 ). The most common solvent used for extraction of residues and contaminants from milk is acetonitrile, which is better than other solvents such as acetone, ethanol and methanol in matrix precipitation.…”

Section: Discussionmentioning

confidence: 99%

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

Hajrulai-Musliu

Uzunov

Jovanov

et al. 2022

Journal of Veterinary Research

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Introduction Multi-class and multi-residue analyses are very complex procedures because of the physico-chemical properties of veterinary drug residues and other contaminants. The purpose of the study was to develop an analytical method for the sensitive determination of 69 analytes in bovine milk by liquid chromatography electrospray ionisation–tandem mass spectrometry. Material and Methods Antimicrobial, anabolic hormone, lactone, β-agonist, mycotoxin and pesticide residues were analysed in 120 raw milk samples from different dairy farms in North Macedonia. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. Results The linear regression coefficients were higher than 0.99, the limits of detection ranged from 0.0036 to 47.94 μg/L, and the limits of quantification ranged from 0.053 to 59.43 μg/L. The decision limit values ranged from 0.062 to 211.32 μg/L and the detection capability from 0.080 to 233.71 μg/L. Average recoveries of the analytes spiked in raw milk were in the range of 70.83% to 109%, intra-day coefficient of variation (CV) values from 2.41% to 22.29%, and inter-day CV values from 3.48% to 23.91%. The method was successfully applied in the testing of bovine milk samples. In five samples residues were detected. They were sulfadimethoxine (in two samples), enrofloxacin, tetracycline and oxytetracycline and were at concentrations below the EU maximum residue limit. Conclusion The method is useful for routine testing for this group of chemical hazards in bovine milk.

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Section: Discussionmentioning

confidence: 99%

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

Hajrulai-Musliu

Uzunov

Jovanov

et al. 2022

Journal of Veterinary Research

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“…Nowadays, chromatographic techniques are widely used to determine steroid hormones. − Among them, high-performance liquid chromatography-tandem mass spectrometry analysis (HPLC-MS/MS) is the dominant strategy for steroid detection. This strategy can not only provide quantitative and qualitative information but also achieve superior sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Click Isotope Mass Probe for Highly Selective Determination of Trace Steroid Hormones in Food Samples

Liu

Zhang

et al. 2022

J. Agric. Food Chem.

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Matrix effects are a great challenge for the quantitative analysis of complex food samples by liquid chromatography-tandem mass spectrometry analysis (LC-MS/MS). Stable isotope labeling (SIL) has been widely used as an effective strategy to eliminate matrix effects. Herein, a copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click-reaction-based SIL method was proposed for a highly sensitive and selective determination of six synthetic steroid hormones in three different food samples (milk, yogurt, and eggs) by high-performance liquid chromatography (HPLC)-MS/MS. A pair of novel SIL agents, N-(2-azidyl ethyl) aniline (d 0-AEA) and d 5-N-(2-azidyl ethyl) aniline (d 5-AEA) were synthesized to label steroid hormones in the samples and standard solution, respectively. The reaction accomplishes in 30 min at 60 °C. The heavy labeled standards were used as internal standards (ISs), which experience the identical ionization processes with light labeled samples to minimize matrix effects. After derivatization, the ionization efficiencies of steroid hormones were greatly improved by 2–54-folds, and the matrix effects ranged from 88.6 to 99.8%. The established method achieved satisfactory detection limits (0.1–2.5 μg L–1) and high recoveries (85–102%). These results demonstrated that the proposed method holds unique advantages for trace steroid hormones analysis in foodstuffs.

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“…ELISA kits had been developed specifically for screening samples glucocorticoid for its sensitivity and rapidity; unfortunately, this method was limited to use in the qualitative detection [4]. The commonly used method of quantitative analysis include gas chromatography [5][6], high performance liquid chromatography [7][8], gas chromatography-mass spectrometry [9,10], liquid chromatography-mass spectrometry [11,12]. Gas chromatography and high performance liquid chromatography cannot be applied to the detection of trace, because of low sensitivity, poor selectivity and the lack of specificity.…”

Section: Introductionmentioning

confidence: 99%

Determination of eight kinds of glucocorticoids residues in chicken muscle with on-line clean up combined HPLC-MS/MS

Ya-hua

Zhi-zhong

Meng

et al. 2022

AChrom

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A sensitive and rapid method using HPLC-MS/MS was developed for the determination of eight glucocorticoids residues in chicken muscle simultaneously by Turbo Flow. The eight glucocorticoids were prednisone, prednisolone, hydrocortisone, methylprednisolone, dexamethasone, betamethasone, beclomethasone and fludrocortisones. Samples were extracted with ethyl acetate and on-line cleaned up through a Turbo Flow solid-phase extraction column without time-consuming pretreatment before HPLC-MS/MS analysis. Sample pretreatment conditions, Turbo Flow conditions and mass spectral parameters were optimized and obtained eight glucocorticoids calibration curves. These curves showed good linearity over the concentration from 0.2 μg/kg to 50 μg/kg with an average recovery from 71.63% to 117.36%. This method could be applied on real samples and provided simple, rapid, sensitive and highly selective analysis, which made it feasible to be adopted in food inspection organizations or carry out quantitative analysis for other banned substance.

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Determination of Hormones Residues in Milk by Gas Chromatography-Mass Spectrometry

Cited by 22 publications

References 22 publications

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

Click Isotope Mass Probe for Highly Selective Determination of Trace Steroid Hormones in Food Samples

Determination of eight kinds of glucocorticoids residues in chicken muscle with on-line clean up combined HPLC-MS/MS

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