Determination of hexachlorobenzene (HCB) in the perirenal and dorsal fatty tissues of pigs

Abstract

The aim of this study was to assess the suitability of the perirenal fatty tissue for the determination of an organochlorine pesticide. Fatty tissue samples were prepared by the matrix solid phase dispersion (MSPD) method, and pesticide levels were determined by gas chromatography on capillary column using an electron capture detector. Results were confirmed by gas chromatography/mass spectrometry (GC/MS) system. The results showed that the perirenal fatty tissue contained significantly higher levels of hexachlorobenzene (HCB) than the dorsal fatty tissue (P < 0.01). All the levels were below the criteria for maximum residue limits established by Croatia and the EU.

Application of matrix solid-phase dispersion to the determination of polychlorinated biphenyls in fat by gas chromatography with electron-capture and mass spectrometric detection

“…Up to now, several authors have used this technique for the determination of CBs in freeze-dried, fresh fish and seafood samples [21,[23][24][25]. Applications to fat samples have been focussed in the extraction of hexachlorobenzene and other chlorinated pesticides [26,27]; however, from our knowledge, the one-step extraction-purification of CBs from pure fat samples, using matrix solid-phase dispersion, has not been reported yet.…”