Determination of Hepcidin Concintration and Many Hematological and Biochemical Variations in Patients With Diabetestype 2 in Balad City

Abstract

Introduction: Diabetes is a group of metabolic diseases characterized by excessive blood sugar caused by a defect in insulin secretion or function, there are two main types of diabetes: namely, insulin-dependent diabetes mellitus (Type I) known as childhood diabetes, The second type is non-insulin-dependent diabetes mellitus, and usually affects adults after the age of forty. Hepcidin is the main organizer of the iron metabolism inHuman and in the rest of mammals.
 The aim of the study:
 
 Examine the relationship between the concentrations of each of the hepcidin, glycated hemoglobin HBA1C and ferritin and iron in patients with type II diabetes as well as healthy control.
 Examine the relationship between these virables with each other and with other virables.
 
 Materials and Methods: The concentration of hepcidin hormone and other biochemical variables was examined in (80) patients with diabetes type II, divided into two groups (39) males and (41) females. Age ranged between (34-75) years. Samples have been collected from patients who visited Balad General hospital laboratory and Dijla Private Laboratory in Balad District. At the same time , blood of (25) healthy volunteers was collected. Sample was divided into 2.5 ml in EDTA tube for hematological tests like complete blood count and HbA1C and 2.5 ml in heparin anticoagulated tube for chemical tests like blood sugar, Iron, Total iron binding capacity and Hepcidin.
 Results and Discussion: The results study showed a significant relationship (P<0.05) between the concentration of Hepcidinin patients with type 2 diabetes and healthy persons for both sexes . Also hepcidin concentration tend to increase in female patients as compared with male patients.
 It was also noted that there was a statistically significant relationship between hepcidin and ferritin concentration (p<0.05), as well as between iron and Hepcidin concentration (p < 0.05), with no statistical relationship between iron binding capacity (TIBC) and the concentration of hepcidin.
 The study showed a Positive correlation between the concentration of Hepcidinand count of red blood cells in people with type 2 diabetes when compared with healthy people.
 Conclusion: The average concentration of hepcidin was found to be higher among females than that among males. Also, there was an increase in the concentration of Hepcidin compared with healthy people.

Relationship of hepcidin with Iron and lipids in patients with polycystic ovary Syndrome

“…Body mass index (BMI) was considered and the patients with polycystic ovary syndrome were divided into three groups according to BMI G1 16-25 kg \ m2 (normal weight), G2 26-35 kg \ m2 (overweight) , G3 36-45 kg\m2 (obesity) these totals were compared to each other. The Table (3) showed increase in the first and second group and a decrease in the third groups .but iron showed increase in the third group and a decrease in the first and second groups .This finding suggested increased body iron stores in these women, raising the possibility that genes related to iron metabolism are altered in polycystic ovarian syndrome [22] . 5) shows an increase in the concentration of hepcidin in the second and third group and its decrease in the first group.…”

“…This hormone plays an important role in regulating the absorption of iron from the gut , as well as in the storage process of iron. [3] It is a peptide made in the liver and discovered in 2000 [4]. The hepcidin is an antimicrobial peptide.…”

“…It is the encoded gene of hepcidin. The prefix-Hep is the designation of hepcidin to the production of this peptide in the liver and which has a pesticide of the carbobacteria referred to by the cidin part [3]. The lipids is a natural substance that dissolves in organic compounds and no dissolves in water.…”