Determination of heat-stable exotoxin of Bacillus thuringiensis by high-performance anion-exchange chromatography

Bubenschikova, S.N.; Kagramanova, V. K.; Baratova, Ludmila A.; Danilova, Elvira B.; Kruglyak, E. B.

doi:10.1016/0022-2011(83)90173-8

Journal of Invertebrate Pathology

1983

DOI: 10.1016/0022-2011(83)90173-8

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Determination of heat-stable exotoxin of Bacillus thuringiensis by high-performance anion-exchange chromatography

S.N. Bubenschikova

V. K. Kagramanova

Ludmila A. Baratova

et al.

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Cited by 3 publications

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“…Several laboratories have reported the use of high-performance liquid chromatography (HPLC) for the detection and quantitation of P-exotoxin (3,24,32), as an alternative to the more tedious and time-consuming fly bioassay (21). Campbell et al developed a method which they report to be both fast and specific for P-exotoxin, even in complex fermentation broths (5).…”

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Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography

et al. 1990

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An improved high-performance liquid chromatography separation was developed to detect and quantify beta-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin production was assigned to a plasmid in five strains, from three subspecies (B. thuringiensis subsp. thuringiensis serotype 1, B. thuringiensis subsp. tolworthi serotype 9, and B. thuringiensis subsp. darmstadiensis serotype 10). A new exotoxin, called type II beta-exotoxin in this report, was discovered in B. thuringiensis subsp. morrisoni serotype 8ab, purified, and partially characterized. This material is more specific than type I beta-exotoxin and is very active against the Colorado potato beetle, Leptinotarsa decemlineata.

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Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography

et al. 1990

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Sample Preparation for β-Exotoxin Determination in Bacillus thuringiensis Cultures by Reversed-Phase High-Performance Liquid Chromatography

Gohar¹,

Perchat²

2001

Analytical Biochemistry

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Development and validation of a confirmative LC-MS/MS method for the determination of ß-exotoxin thuringiensin in plant protection products and selected greenhouse crops

Rijk¹,

Dam²,

Zomer³

et al. 2012

Anal Bioanal Chem

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Bacterial products based on Bacillus thuringiensis are registered in many countries as plant protection products (PPPs) and are widely used as insecticides and nematocides. However, certain B. thuringiensis strains produce harmful toxins and are therefore not allowed to be used as PPPs. The serotype B. thuringiensis thuringiensis produces the beta-exotoxin thuringiensin (ßeT) which is considered to be toxic for almost all forms of life including humans (WHO 1999). The use of a non-registered PPP based on B. thuringiensis thuringiensis called bitoxybacillin was established through the determination of ßeT. First, an analytical reference standard of ßeT was characterized by nuclear magnetic resonance, liquid chromatography-high-resolution mass spectrometry and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Then, a confirmatory quantitative method for the determination of ßeT in PPPs and selected greenhouse crops based on LC-MS/MS was developed and validated. A limit of quantitation of 0.028 mg/kg was established, and average recoveries ranged from 85.6 % to 104.8 % with repeatability (RSDr) of 1.5-7.7 % and within-lab reproducibility (RSD(WLR)) of 17 %. The method was used for analysis of >100 samples. ßeT was found in leaves of ornamentals, but no evidence was found for use in edible crops.

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Determination of heat-stable exotoxin of Bacillus thuringiensis by high-performance anion-exchange chromatography

Cited by 3 publications

References 8 publications

Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography

Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography

Sample Preparation for β-Exotoxin Determination in Bacillus thuringiensis Cultures by Reversed-Phase High-Performance Liquid Chromatography

Development and validation of a confirmative LC-MS/MS method for the determination of ß-exotoxin thuringiensin in plant protection products and selected greenhouse crops

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