Determination of HBsAg subtypes in different high risk populations using monoclonal antibodies

Swenson, Paul D.; Riess, John T.; Krueger, Leigh

doi:10.1016/0166-0934(91)90004-j

Cited by 39 publications

(31 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This explains why in general subtypes expressing r do not react with antisera recognizing wl to w4, and it also explains the pseudoallelic relationship between the subdeterminants of w and r. Thus, there are several indications that the epitope for subdeterminants of w resides at positions 125 to 127. However, it was not possible to identify any variation between the sequences of P1 and P2 in this region, in agreement with the difficulties in discriminating between aywl and ayw2 with monoclonal antibodies (Swenson et al, 1991).…”

Section: Discussionmentioning

confidence: 54%

Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains

Nordér¹,

Hammas²,

Löfdahl³

et al. 1992

Journal of General Virology

335

278

View full text Add to dashboard Cite

The surface (S) genes of 12 hepatitis B viruses (HBVs) encoding nine different serotypes of hepatitis B surface antigen (HBsAg) were amplified by the polymerase chain reaction and sequenced. These represented the eight strains of HBV, PI to P8, defined at an international workshop on HBsAg subtypes in Paris in 1975, and the adrq-subtype. The S genes from additional HBV strains, one ayw4, one adw4 and one aywl, of sub-Saharan African origin, were also sequenced. The relationship of these 12 new S gene sequences to those of the 20 published previously was investigated by constructing a phylogenetic tree, which confirmed a previous classification into four groups, designated A to D, based on 18 complete HBV genomes. When relating our sequenced S genes to these genomic groups, aywl of African origin and P6 (adw2) were both allocated to group A, the reference P1 (aywl of Vietnamese origin) was allocated to group B, P5 (ayr), P8 (adr) and adrq-were all related to group C, and P2 (ayw2) and P3 (ayw3) could both be allocated to group D. Interestingly, the S genes of w4 serotype viruses, i.e. P4 (ayw4) and P7 (adw4q-), differed by 4% or more from both previous groups and from each other, suggesting their classification into two new groups, for which the designations E and F are proposed. Genomes specifying ayw were also found in groups A and B; previously sequenced genomes specifying the ayw subtype have all been confined to group D. There were indications that the epitope for subdeterminants of w resided at amino acid positions 125 to 127. Thus, at positions 125 and 127, aywl, ayw2 and adw2 had T and P residues, respectively, whereas M and T residues were at the corresponding positions ofayw3. Both ayw4 and adw4 had L at residue 127, and all strains expressing r, apart from P5, had an I instead of a T residue at position 126.

show abstract

Section: Discussionmentioning

confidence: 54%

Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains

Nordér¹,

Hammas²,

Löfdahl³

et al. 1992

Journal of General Virology

335

278

View full text Add to dashboard Cite

show abstract

“…The concept that there are at least nine different HBV serotypes is now widely accepted (Courouc6 et al, 1976;Wands et al, 1984;Ben-Porath et al, 1985;Swenson et al, 1991). The molecular mapping of the subdeterminants defining these serotypes is important for several reasons.…”

Section: Resultsmentioning

confidence: 99%

Molecular basis of hepatitis B virus serotype variations within the four major subtypes

Nordér

Couroucé

Magnius

1992

Journal of General Virology

177

148

View full text Add to dashboard Cite

Amino acid residues 101 to 180 of hepatitis B surface antigen (HBsAg) were predicted by sequencing the corresponding part of the S gene of hepatitis B virus (HBV) DNA in 46 HBsAg-positive sera, which had been subtyped by immunodiffusion with respect to d/y, w/r, wl to w4 and q. The sequences of the nine different HBV serotypes defined by these specificities were found to be homogeneous proving that they represent consistent variations of HBV at the genomic level. Residue 127 was found to be important as were Pro, Thr and Leu for wl/w2, w3 and w4, respectively. Five residues were found to differ between aywl and ayw2. These were at positions 134 (Phe instead of Tyr), 143 (Thr instead of Ser), 159 (Ala instead of Gly), 161 (Tyr instead of Phe) and 168 (Val instead of Ala). However, all these residues were shared by aywl and adw2, implying that Arg 122 was also important for wl expression. All genomes expressing r, apart from one ayr strain, had an Ile 126, which might explain the pseudo-allelism of wl to w4 in relation to r, since this substitution might influence the w epitope. There were two regions where adw4q-and adrq-differed from all the q÷ subtypes. These were located at residues 158 and 159, and at residues 177 and 178, where both the qsubtypes had amino acid substitutions in adjacent positions. The mapping of the epitopes defining these antigenic specificities will help to link information on the world-wide distribution of HBsAg subtypes to future molecular epidemiology with regard to HBV.

show abstract

“…Testing for the presence of HBsAg, the main serological marker of HBV, in donor blood is obligatory virtually in all countries of the world. The test kits for detection of this antigen should be capable of recognizing all its subtypes discovered so far [Courouce et al, 1976[Courouce et al, , 1978[Courouce et al, , 1983Wands et al, 1984;Swenson et al, 1991]. In addition, during the last decade numerous data have been obtained on the occurrence of mutations in S gene that cause certain changes in HBsAg [Burda et al, 2001;Hou et al, 2001;Torresi, 2002].…”

Section: Introductionmentioning

confidence: 98%

Determination of HBsAg subtypes in Western Siberian part of Russia

Нетесова

Swenson

Osipova

et al. 2003

Journal of Medical Virology

View full text Add to dashboard Cite

A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2:8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg-positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2:58 (15 ayw3varA and 43 ayw3varB; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3:(both variants, 33 ayw3varA and 1 ayw3varB; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk.

show abstract

Determination of HBsAg subtypes in different high risk populations using monoclonal antibodies

Cited by 39 publications

References 21 publications

Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains

Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains

Molecular basis of hepatitis B virus serotype variations within the four major subtypes

Determination of HBsAg subtypes in Western Siberian part of Russia

Contact Info

Product

Resources

About