Determination of Hæmoglobin

Methods of Routine Haemoglobinometry in Childhood: Evaluation of Copper Sulphate Densitometry

“…Macfarlane, King, Wootton, and Gilchrist (1948) found an even greater error in the Sahli method than that reported here. The error of photoelectric colorimetry of cyanmethaemoglobin in this study was similar to that reported by King, Bartholomew, Geiser, Ventura, Wootton, Macfarlane, Donaldson, and Sisson (1951) but somewhat greater than that reported earlier by the same workers (King, Gilchrist, Wootton, Donaldson, Sisson, Macfarlane, Jope, O'Brien, Peterson, and Strangeways, 1948) and by Macfarlane et al (1948). Since their studies were performed on pooled venous blood rather than capillary samples, their smaller errors are not surprising.…”

Haemoglobin Levels

“…The colour of the mucous membranes, as reported elsewhere (Adcock et al, 1949) may fail as an index of Hb level, but the Tallqvist method provides a simple and inexpensive means of ascertaining the Hb level with sufficient accuracy for practical purposes. This method was found by Macfarlane et al (1948) The administration of dicoumarol (3,3'-methylene-bis-(4-hydroxycoumarin)) or "tromexan" (bis-3,3'-(4-oxycoumarinyl)-ethyl acetate) in the treatment of thromboembolic states may lead to severe or even fatal haemorrhage. Although careful control of treatment by repeated estimation of the blood prothrombin levels reduces this risk, a reliable and convenient antidote against such potent therapeutic substances would be of great value.…”

Cycloguanil Pamoate in the Treatment and Suppression of Malaria in the Gambia, West Africa

“…Oxyhaemoglobin values were determined from capillary blood with the M.R.C. grey-wedge photometer (Macfarlane et al, 1948). Microhaematocrit readings were made after spinning in a Hawksley microhaematocrit centrifuge -for five minutes at 12,000 g. Thin blood films were stained by Giemsa's method and thick ones by Field's.…”