Determination of haematology and blood chemistry values in healthy six‐week old broiler hybrids

Ross, J. G.; Christie, Gail E.; Halliday, W.G.; Jones, R. Morley

doi:10.1080/03079457608418196

Cited by 22 publications

(11 citation statements)

References 11 publications

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“…The total red blood cell (RBC) and white blood cell (WBC) count were determined using improved haemocytometer method as described by Lamb [ 11 ]. Differential WBC count was determined by preparing blood smear stained with Wrights stain as described by Ross et al [ 12 ]. Haemoglobin concentration was estimated using cyanomethaemoglobin method as described by Jain [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Sweet Orange Fruit Waste Diets and Acidifier on Haematology and Serum Chemistry of Weanling Rabbits

Daudu

Sani

Adedibu

et al. 2014

Journal of Veterinary Medicine

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A total of thirty-five mixed breed (35) rabbits of average weight of 700 g aged 5-6 weeks were allocated to seven treatments in a completely randomised design to investigate the effect of sweet orange fruit waste (SOFW) and acidomix acidifier on haematology and serum chemistry. The diets were 0% SOFW, 10% SOFW with 0.5% acidomix, 10% SOFW with 0.7 acidomix, 15% SOFW with 0.5% acidifier, 15% SOFW with 0.7% acidifier, 20% SOFW with 0.5% acidifier, and 20% SOFW with 0.7% acidifier. Blood samples were analyzed for haemoglobin (hb) concentration, white blood cells (WBC), red blood cells (RBC), differential WBC count (lymphocyte, basophil, eosinophil, monocyte, and neutrophil), alanine amino transferase (ALT), alkaline phosphatase (ALP), aspartate amino transferase (AST), total protein, albumin, and globulin. There was no interaction between SOFW and acidifier for the haematological and most of the serum chemistry parameters but significant difference was observed in ALT; however the values were within the normal range. SOFW had no significant effect on all haematological and serum chemistry parameters. Acidomix had significant effect (P < 0.05) on haemoglobin concentration; rabbits fed 0.5% acidomix diets had higher values which were within the normal range. It is therefore concluded that SOFW with acidifier up to 20% had no detrimental effect on serum chemistry and haematology.

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Section: Methodsmentioning

confidence: 99%

Effect of Sweet Orange Fruit Waste Diets and Acidifier on Haematology and Serum Chemistry of Weanling Rabbits

Daudu

Sani

Adedibu

et al. 2014

Journal of Veterinary Medicine

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“…Hematological and biochemical parameters have been studied in avian species for many decades. Ross et al. (1976) utilized a variety of bench top analytical procedures to determine 14 different “comparison values” for 21 broiler flocks comprising over 900 samples at 42 d of age ( Ross et al., 1976 ).…”

Section: Introductionmentioning

confidence: 99%

“… Ross et al. (1976) utilized a variety of bench top analytical procedures to determine 14 different “comparison values” for 21 broiler flocks comprising over 900 samples at 42 d of age ( Ross et al., 1976 ). While Meluzzi et al.…”

Section: Introductionmentioning

confidence: 99%

“…It has been established that between flock variance of hematological comparison values has a significantly greater variance than within flock variance ( Ross et al., 1976 ). Therefore, the objective of this study was to determine reference ranges for the i-STAT® Handheld Clinical Analyzer, Zoetis Vetscan VS2, and iCheck™ Carotene over the typical growth phases for similar Ross and Cobb modern fast-growing high yielding broilers of differing sexes raised concurrently.…”

Section: Introductionmentioning

confidence: 99%

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Effect of broiler genetics, age, and gender on performance and blood chemistry

Livingston¹,

Cowieson

Crespo

et al. 2020

Heliyon

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A total of 640 broilers were used to determine the effects of strain, sex, and age on hematology and blood chemistry using rapid detection devices. Day old chicks from two genetic lines of common fast-growing and high-yield broiler strains were sexed and allocated to 40 pens (16 birds per pen) containing either male or female and Ross or Cobb strains (n = 10). Venous blood was analyzed weekly using 2 broilers from each pen (n = 20) using the i-STAT® Alinity Handheld Clinical Analyzer, Zoetis Vetscan VS2, and iCheck™ Carotene devices at 14, 21, 28, 35, and 42 d, as well as growth performance. Post-mortem health tracking metrics were also recorded on 42 d. Broilers were deemed healthy based on posting data results and performed in accordance with industry standards with males presenting greater BW and reduced FCR than female broilers. Ross broilers displayed greater BW to 14 d with similar FCR compared with Cobb birds. Day of age had a highly significant impact on blood calcium, phosphorus, potassium, sodium, chloride, carotene, aspartate aminotransferase, creatine kinase, bile acids, uric acid, total protein, albumin, globulin, total carbon dioxide, hematocrit, and malondialdehyde. Male broilers had reduced blood sodium, chloride, carotene, uric acid, albumin, and increased total protein, glucose, and total carbon dioxide. Ross broilers had greater blood potassium, and sodium, as well as reduced uric acid, total protein, globulin, and malondialdehyde, compared with Cobb birds. These results demonstrated the effectiveness of point-of-care devices in measuring blood chemistry and hematology in modern broilers. These data can be utilized to determine normal healthy blood ranges in these types of broilers when accounting for strain, sex, and age.

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“…The results of the biochemical and haematological examination proved that pseudomonas infection has been associated with significant decrease in RBC's count Hb concentration and PCV% while there was an increased TLC (Lymphocytosis and heterophilia) which might be due to the antigenic stimulation of Pseudomonas aeruginosa which leads to an increase in the thymus dependent lymphocytes (Tlymphocytes) production as reported (Coles, 1986). Also, there was an elevation in the serum activities of ALT, AST and AP which seem probably to be due to the liver damage by the effect of Pseudomonas aeruginosa toxins following the escape of the enzymes into the serum in an abnormal high level (Eisa, 1998;Ross et al, 1976). The results were confirmed by the histopathological findings.…”

Section: Discussionmentioning

confidence: 80%

Treatment trials of Pseudomonas aeruginosa infection in quails

Badr¹,

Metwali²,

Yoseif³

et al. 2006

Journal of Veterinary Medical Research

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An evaluation of the efficacy of different treatment trials in controlling Pseudomonas aeruginosa infection in quail chicks was done. Two probiotics (Lactobacilli and active dry yeast) and two fluoroquinolone antibiotics (Ciprofloxacin & ofloxacin) were used. Four-daysold quail chicks were infected orally with 0.2ml containing 3 x 108 CFU/ml of Pseudomonas aeruginsa strain which proved to be highly pathogenic for quails and sensitive to both antibiotics mentioned above. After three days, infected quails were sorted out into treated and control groups. The fluoroquinolone treated groups were given either of the antibiotics in the drinking water at a dose level of 10mg/kg BW every 12 h for five successive days, while the probiotic treated groups received either and both of the probiotics at a dose level of 0.5gm/liter daily till the end of the experiment. The results revealed that fluoroquinolones were highly effective in destroying the infectious agent. on the other hand, the general health condition and body functions were negatively affected. Histopathological changes in fluoroquinolone and probiotic treated groups were similar. Bacteriologically, the probiotics failed to remove pseudomonas from the infected internal organs. Probiotics proved to be effective in reducing mortalities, severity of infection, enhancement of liver and kidney functions up to almost normal values and improving quail immunity. Probiotics resulted in improvement of the haematological and biochemical parameters as well as total serum proteins and serum globulins. Dual administration of the lactobacilli and saccharomyces justified their synergistic probiotics effect against quail chick pseudomoniasis.Abbreviations: ALT= alanine aminotransferase; AP= alkaline phosphatase; AST= aspartate amintransferase; B= basophils; CFU= colony forming unit CFU/ml = colony forming unit per millilitre; Cipro.= ciprofloxacin; E= eosinophils; gm/ Liter = gram per liter; H= heterophils; Hb concentration = haemoglobin concentration; Hb%= haemoglobin percentage; L= lymphocytes; Lact.=lactobacillus; L. acidophilus= lactobacillus acidophilus; L. brevis= lactobacillus brevis; L. planterum= lactobacillus planterum; M= monocytes; MIC= minimum inhibitory concentration; Of= ofloxacin; PCV= packed cell volume; pH= hydrogen ion concentration; Ps= pseudomonas aeruginosa; R= resistant; TLC= total leucocytic count; T. lymphocytes = thymus dependent lymphocytes; Y= active dry yeast.

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Determination of haematology and blood chemistry values in healthy six‐week old broiler hybrids

Cited by 22 publications

References 11 publications

Effect of Sweet Orange Fruit Waste Diets and Acidifier on Haematology and Serum Chemistry of Weanling Rabbits

Effect of Sweet Orange Fruit Waste Diets and Acidifier on Haematology and Serum Chemistry of Weanling Rabbits

Effect of broiler genetics, age, and gender on performance and blood chemistry

Treatment trials of Pseudomonas aeruginosa infection in quails

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