Determination of green tea catechins in human plasma using liquid chromatography–electrospray ionization mass spectrometry

Masukawa, Yoshinori; Matsui, Yuji; Shimizu, Namii; Kondou, Naoki; Endou, Hidenori; Kuzukawa, Michiya; Hase, Tadashi

doi:10.1016/j.jchromb.2006.02.008

Cited by 60 publications

(50 citation statements)

References 31 publications

(101 reference statements)

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“…2 presents the optimal chromatograms obtained with the four stationary phases. Firstly, it is worth mentioning that the elution order of non-epi and epi forms was identical for all investigated columns and was in close agreement with previous studies [36,37]. Indeed, epi forms with gallate (EGCG and ECG) were eluted prior to non-epi forms with gallate (GCG and CG), and a non-epi form without gallate (C) was eluted prior to non-epi forms without gallate (EGC and EC).…”

Section: Comparison Of Various Columns Packed With Sub-2 M Particlessupporting

confidence: 89%

High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors

Guillarme

Casetta

Bicchi

et al. 2010

Journal of Chromatography A

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a b s t r a c tThe analysis of polyphenols in tea extracts is important due to their potential health benefits. Therefore, efficient and high throughput analytical methods have been developed for the separation of seven predominant polyphenols, also known as catechin derivatives, present in tea extracts. Columns packed with sub-2-m particles operating at elevated pressure (UHPLC strategy) were selected to improve chromatographic performance. The potential of UHPLC-UV was demonstrated with baseline resolution of all standard catechins in only 30 s using a 50-mm column packed with 1.7-m particles. When dealing with real samples such as tea extracts, however, longer columns of up to 150 mm in length were employed to enhance the separation of catechin derivatives and other constituents within the tea samples while maintaining an acceptable analysis time. Two strategies based on 2-D experiments were proposed to clearly identify catechins. Firstly, a liquid-liquid extraction procedure was added prior to the UHPLC-UV analysis to decrease the complexity of the sample. Secondly, UHPLC was coupled to ESI-MS/MS to attain sufficient sensitivity and selectivity between catechin derivatives and other constituents of tea extract. These two strategies were found extremely promising as a clear discrimination of catechins from the matrix could be attained.

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Section: Comparison Of Various Columns Packed With Sub-2 M Particlessupporting

confidence: 89%

High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors

Guillarme

Casetta

Bicchi

et al. 2010

Journal of Chromatography A

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“…In contrast to the fact that no quercetin aglycone was detected in human plasma, tea catechins, especially ECg and EGCg, were detected as aglycones (at 20 and 60 ng/mL respectively, at maximum concentrations 1-2 h after ingestion) in human plasma after the intake of green tea. 28) Polyphenols and their metabolites have been detected in tissues and organs, [29][30][31] but only limited information on their distribution and molecular dynamics is available. In particular, in spite of their potent protective effects in the development of atherosclerosis, little is known about the aortic distribution of polyphenols.…”

Section: Bioavailability Of Polyphenols and Their Activitymentioning

confidence: 99%

“…In particular, plasma concentrations are important indexes in comparing the effective concentrations obtained from in vitro experiments with in vivo situation. Several human studies have indicated that plasma concentrations in humans after intake of polyphenols reach maximum concentrations of less than a few mM, 28,57,58) but there are often discrepancies between plasma concentrations and effective concentrations in vitro. In most in vitro experiments, higher concentrations of polyphenols (>5-10 mM) are required to bring about beneficial effects.…”

Section: Perspectives: From Bioavailability To Target Moleculesmentioning

confidence: 99%

Immunochemical Detection of Food-Derived Polyphenols in the Aorta: Macrophages as a Major Target Underlying the Anti-Atherosclerotic Activity of Polyphenols

Kawai

2011

Bioscience, Biotechnology, and Biochemistry

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“…A catequina é um composto fenólico que tem grande interesse dos pesquisadores devido à ação antioxidante encontrada em frutas, chás, vinhos, e, consequentemente, seu efeito fisiológico benéfico à saúde [1][2][3]. As catequinas são os membros mais importantes da classe dos flavonoides, elas foram reconhecidas como um antioxidante potente e uma destruidora de radicais livres [4].…”

Section: Introductionunclassified

“…Os métodos tradicionais para a detecção de catequinas incluem cromatografia líquida de alta eficiência (HPLC) [1,16,17] combinada com quimiluminescência [18,19], espectrofotometria [2], eletroquímica [3,[20][21][22][23][24][25][26], UV e detecção fluorimétrica [27].…”

Section: Introductionunclassified

Determinação de catequinas por eletrodo de carbono impresso modificado com nanotubo de carbono funcionalizado

Silva

Kubota

et al. 2016

Eclet. Quim.

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____________________________________________________________________________Resumo: A Catequina é um composto fenólico com grande potencial oxidante, encontrada em diversos tipos de alimentos, dentre alguns dos benefícios da catequina estão o fortalecimento capilar, melhoria do fluxo sanguíneo, redução dos níveis de colesterol. Neste artigo, utilizou-se da técnica de voltametria cíclica (VC) para otimizar o eletrodo de carbono impresso modificado com nanotubo de carbono funcionalizado para detectar e quantificar catequina por meio da técnica de voltametria de onda quadrada (VOQ). Para este eletrodo, os melhores resultados foram obtidos a uma concentração de 1x10 -3 mol L -1 de catequina em meio de tampão fosfato 0,1 mol L -1 pH 7,0 a uma velocidade de varredura de potencial de 0,05 V s -1 . A partir destas condições foi obtida uma curva analítica com comportamento linear no intervalo de concentração de catequina entre 2,0x10 -7 a 1,68x10, com limites de detecção e de quantificação de 9,2x10 -8 e 3,0x10 -7 mol L -1, respectivamente por VOQ. Este eletrodo apresentou boa repetibilidade com um desvio de padrão relativo de 4,05%. A oxidação da catequina apresentou um pico localizado em torno de 0,15V vs Ag/AgCl, estando próximo dos potenciais de pico mostrados na literatura.Palavras-chave: catequina, eletrodo de carbono impresso, nanotubo de carbono, sensor eletroquímico ____________________________________________________________________________ Abstract: Catechin is a phenolic compound with high oxidizing potential, found in several types of foods, some of the benefits of catechins are capillary strengthening, improvement of blood flow and reduction of cholesterol levels. In this paper, it was used the cyclic voltammetry technique (CV) to optimize the printed carbon electrode modified with carbon nanotube, functionalized to detect and quantify catechin by means of the squarewave voltammetry technique (SWV). For this electrode, the best results were obtained at a catechin concentration of 1x10 -3 mol L -1 in means of 0.1 mol L -1 phosphate buffer pH 7.0 at a potential scan rate of 0.05 V s -1 . From these conditions, an analytical curve was obtained with a linear behavior in the catechin concentration range of 1,68x10 -5 to 2,0x10 -7 mol L -1, with limits of detection and quantification of 9,2x10 -8 and 3,0x10 -7 mol L -1 , respectively, by SWV. This electrode showed good repeatability with a standard deviation of 4.05%. Oxidation of catechin showed a peak located around 0,15V vs Ag / AgCl, being close to the peak potentials shown in the literature.

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Determination of green tea catechins in human plasma using liquid chromatography–electrospray ionization mass spectrometry

Cited by 60 publications

References 31 publications

High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors

High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors

Immunochemical Detection of Food-Derived Polyphenols in the Aorta: Macrophages as a Major Target Underlying the Anti-Atherosclerotic Activity of Polyphenols

Determinação de catequinas por eletrodo de carbono impresso modificado com nanotubo de carbono funcionalizado

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