High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors

Abstract

a b s t r a c tThe analysis of polyphenols in tea extracts is important due to their potential health benefits. Therefore, efficient and high throughput analytical methods have been developed for the separation of seven predominant polyphenols, also known as catechin derivatives, present in tea extracts. Columns packed with sub-2-m particles operating at elevated pressure (UHPLC strategy) were selected to improve chromatographic performance. The potential of UHPLC-UV was demonstrated with baseline resolution of all standard catechins in only 30 s using a 50-mm column packed with 1.7-m particles. When dealing with real samples such as tea extracts, however, longer columns of up to 150 mm in length were employed to enhance the separation of catechin derivatives and other constituents within the tea samples while maintaining an acceptable analysis time. Two strategies based on 2-D experiments were proposed to clearly identify catechins. Firstly, a liquid-liquid extraction procedure was added prior to the UHPLC-UV analysis to decrease the complexity of the sample. Secondly, UHPLC was coupled to ESI-MS/MS to attain sufficient sensitivity and selectivity between catechin derivatives and other constituents of tea extract. These two strategies were found extremely promising as a clear discrimination of catechins from the matrix could be attained.

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

“…Many analytical methods have been described for determination of flavonols in wines. The most selective methods involve often liquid or gas chromatography coupled to detection by mass or tandem mass spectrometry [35][36][37][38][39][40][41][42][43][44][45][46][47][48].…”

A fast method using a new hydrophilic–lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines

“…As an alternative, an ultra-fast UHPLC method appears recently to overcome some of the LC drawbacks retaining the principles of LC while offering some clear benefits in terms of analysis time, resolving power, solvent consumption and, to a lesser extent, sensitivity [25,13]. Up to now, only a few applications using UHPLC have been reported for the analysis of polyphenols (Table 1).…”

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

“…Quantitative analysis of (E)-resveratrol is commonly performed by means of high-performance liquid chromatography (HPLC) connected to a diode array detector or a triple quadrupole mass spectrometer [26,27,[41][42][43][44][45][46][47][48]. In order to increase the selectivity and sensibility of the analytical method the analysis of these kinds of compounds should be performed by using a fluorescence detector.…”