Determination of glucose in soft drink and sugar-cane juice employing a multicommutation approach in flow system and enzymatic reaction

Kronka, Eloisa A.M.; Paim, Ana Paula S.; Reis, Boaventura F.; Lima, José L.F.C.; Lapa, Rui A. S.

doi:10.1007/s002160051349

Cited by 8 publications

(4 citation statements)

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“…Methods A-D, employing FIA and multi-commutation system, are based on the spectrophotometric detection. Light absorption was applied to detect the product after sugar was chemically reacted with oxidizing agent [8][9][10] or enzyme [13] with well-known chemical reactions. Method E employs the FIA-gravimetric detection by online weighing of filtered copper(I) oxide precipitates after total reducing sugar was oxidized by Fehling reagent [11].…”

Section: Comparison With Other Flow-based Methods For Analysis Of Sugarcane Juicementioning

confidence: 99%

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Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry

2021

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This work presents, for the first time, a new sequential injection analysis (SIA) method to simultaneously analyze degree Brix, orthophosphate and pH in raw cane juice. These key parameters relate to price of harvested sugarcane and quality of cane juice for sugar production. The SIA system employed two detectors: the first detector is a diode-array spectrophotometer, equipped with a regular flow cell, for measurements of degree Brix and orthophosphate. Quantitative of degree Brix (°Bx; ca. % (w/w) sucrose) was based on manipulation of the schlieren effect at the interface between plugs of sample and water. Orthophosphate analysis was carried out based on the molybdenum blue method with significant reduction in consumption of the reagents. Compensation of the schlieren effect from sucrose for determination of orthophosphate was achieved by using a dual-wavelength spectrometric detection. Second detector is a pH-sensing device, called ion-selective field-effect transistors (ISFET). The ISFET is based on the current through the ISFET arising according to the H+ concentration in solution. Our developed SIA system provides linear calibration graphs fitting for purpose in analysis of sugarcane juice (pH: 0–14, °Bx: 1.0–7.0 and P2O5: 20–200 mg L−1). Simultaneous analysis of sugarcane juice for pH, °Bx and P2O5 is carried out within 5 min (12 sample per h). Precision of SIA system is acceptable (RSD < 3%). Our SIA system gave quantitative results insignificantly different, as compared with conventional methods for analysis of pH, °Bx and P2O5 in sugarcane juice.

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Section: Comparison With Other Flow-based Methods For Analysis Of Sugarcane Juicementioning

confidence: 99%

“…Sucrose content changed the frequency of the 10 MHz piezoelectric quartz crystal due to variation of sample viscosity and density. In addition, a multi-commutation flow-based approach [13] was also developed for glucose analysis employing enzymatic reaction with glucose-oxidase.…”

Section: Introductionmentioning

confidence: 99%

Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry

2021

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“…However, the high detection limits obtained together with the periodical recalibration of the flow network due to the progressive aging of Tygon tubing hindered its applicability to low-sugar-content samples and restricted its monitoring capabilities. 15 To the best of our knowledge, no automated flow system able to determine trace concentrations of glucose in complex samples using soluble enzymes has been reported so far.…”

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confidence: 99%

“…Despite these shortcomings, many analytical procedures demand the application of soluble enzymes, owing to the complexity of the sample matrix, which might in turn reduce drastically the lifetime of immobilized enzyme-containing columns/membranes, to the lack of proper immobilization capabilities of certain enzyme preparations or to the blockage of the active sites during the retention procedure, making them inaccessible for the substrate. , Recently, the multicommuted flow injection concept has been presented as a powerful tool to handle minute volumes of enzymes in homogeneous phase, as demonstrated by the determination of glucose in soft drinks. However, the high detection limits obtained together with the periodical recalibration of the flow network due to the progressive aging of Tygon tubing hindered its applicability to low-sugar-content samples and restricted its monitoring capabilities . To the best of our knowledge, no automated flow system able to determine trace concentrations of glucose in complex samples using soluble enzymes has been reported so far.…”

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Automated Enzymatic Assays in a Renewable Fashion Using the Multisyringe Flow Injection Scheme with Soluble Enzymes

et al. 2003

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In this paper, a novel flowing stream scheme based upon the multisyringe flow injection (MSFI) technique is presented as a powerful tool to perform automated enzymatic assays. The exploitation of enzymes in homogeneous phase circumvents typical drawbacks associated with the commonly used packed-bead or open tubular permanent columns, namely, malfunctions of the reactor, carryover effects, flow resistance, loss of binding sites, large reagent consumption, and use of harmful organic solvents during immobilization procedures. The proposed MSFI system is able to handle minute volumes of soluble enzymes and accommodate reactions with divergent kinetic and pH demands, as demonstrated via the indirect chemiluminescence determination of trace levels of glucose. The procedure is based on the on-line glucose oxidase-catalyzed oxidation of beta-glucose in homogeneous phase to beta-glucono-delta-lactone and hydrogen peroxide. Subsequently, the generated oxidant merges downstream with an alkaline slug of 3-aminopthalhydrazide and a metal-catalyst zone (viz., Co(II)) at a total flow rate as high as 72 mL/min aiming to warrant maximum light collection from the fast CL reaction. Under optimum conditions for both sequentially occurring reactions, a glucose concentration as low as 90 microg/L may be easily detected at a 1000-fold photomultiplier gain. A second-order polynomial regression equation of light emission versus substrate concentration is found over the range 90 microg/L-2.7 mg/L glucose, although a maximum concentration of 180 mg/L may be determined by suitable gain selection without requiring manifold reconfiguration. An injection throughput of 20 h(-1), a repeatability better than 2.5% at the 1 mg/L level, and a 3sigma detection limit of 72 microg/L are the analytical features of the designed analyzer. The proposed approach was applied to the analysis of ultralow glucose content soft drinks as well as fruit juices suitable for diabetic consumers. The accuracy was assessed using the spectrophotometric batch glucose-Trinder method as an external reference methodology for the determination of the target species in parenteral solutions.

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Microfluidic paper-based analytical device for determination of sucrose in sugarcane juice using Benedict’s reagent

et al. 2022

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Determination of glucose in soft drink and sugar-cane juice employing a multicommutation approach in flow system and enzymatic reaction

Cited by 8 publications

References 0 publications

Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry

Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry

Automated Enzymatic Assays in a Renewable Fashion Using the Multisyringe Flow Injection Scheme with Soluble Enzymes

Microfluidic paper-based analytical device for determination of sucrose in sugarcane juice using Benedict’s reagent

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