Determination of gentamicin in bovine milk using liquid chromatography with post-column derivatization and fluorescence detection

Kijak, Philip James; Jackson, Jean; Shaikh, Badar

doi:10.1016/s0378-4347(96)00445-8

Cited by 34 publications

(31 citation statements)

References 12 publications

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“…The detection limit in milk was 600 ppb. 18 A regulatory assay for gentamicin in milk at 30 ppb was described by Kijak et al 19 Milk was defatted, then deproteinated with trichloroacetic acid. After isolation by C 18 solid phase extraction, gentamicin was eluted with ammonia solution in methanol.…”

Section: Extractionmentioning

confidence: 99%

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Confirmation of gentamicin and neomycin in milk by weak cation-exchange extraction and electrospray ionization/ion trap tandem mass spectrometry

2000

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Section: Extractionmentioning

confidence: 99%

“…This milk had been assayed by the determinative method of Kijak et al 19 during a method trial. The dosing regimen and the depletion study are described elsewhere.…”

Section: Validationmentioning

confidence: 99%

Confirmation of gentamicin and neomycin in milk by weak cation-exchange extraction and electrospray ionization/ion trap tandem mass spectrometry

2000

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“…A chromatogram of blank corn meal and a colistin-containing (20 mg kg -1) feed extract is shown on Figure 1. An assay method for the determination of gentamicin, neomycin, kanamycin and amikacin was developed by optimization of the procedures described in the literature [10,11]. During optimization, two columns were tested and good results were obtained both for the TSK ODS 120T and the Hypersil ODS columns.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Determination of gentamycin by pre-column derivatization using OPA-mercaptopropionic acid is the official method for the determination of gentamycin congeners in the European Pharmacopoeia [2]. Determination of gentamicin in bovine milk was carried out by post-column derivatization using OPA-2--mercaptoethanol [11]. Analysis of colistin and bacitracin by reversed-phase LC and post-column denvatization has also been reported [12].…”

Section: Introductionmentioning

confidence: 99%

HPLC determination of colistin and aminoglycoside antibiotics in feeds by post-column derivatization and fluorescence detection

Morovján¹,

Csokan²,

Németh-Konda³

1998

Chromatographia

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SummaryReversed-phase, HPLC methods employing postcolumn derivatization and fluorescence detection were developed for the determination of the peptide colistin and four aminoglycoside antibiotics in feeds. Extraction of the analytes was by sonication and shaking with dilute hydrochloric acid. Post-column derivatization was performed using orthophtaldialdehyde-2-mercaptoethanol chemistry. Assay of colistin was by using an acetonitrile-aqueous sodium sulphate-triethylammonium phosphate (pH 2.8) eluent. Aminoglycoside antibiotics:amikacin, kanamycin, gentamycin and neomycin were analyzed using a tetrahydrofuran-aqueous sodium sulphate-sodium pentanesulphonate-acetic acid mobile phase. The method was also applied to some pharmaceutical preparations. Preliminary results showed that the method can be adapted for the assay of the above antibiotics in meat and animal serum for residue and pharmacokinetic studies.

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“…The advantages of gentamicin over other aminoglycosides (kanamycin, neomycin) are its activity towards Pseudomonas aeruginosa and microorganisms of the Serratia-Klebsiella -Enterobacter group, a faster bactericidal effect, and the rare development of gentamicin resistant strains [1]. It can be quantified using colorimetric technique [2], amperometric enzyme-immunosensors [3], flow injection chemiluminescence [4], spectrofluorometry [5][6][7], capillary electrophoresis with UV detection [8], liquid chromatography (LC) [9][10][11][12][13][14] and high performance liquid chromatography (HPLC) [15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

et al. 2006

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Four simple, accurate, sensitive and economical procedures (A -D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λ max =521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4-8, 3-8, 4-9 and 5-9 µg ml −1 with gentamicin and 4-8, 1.5-4, 1.5-4 and 3.5-5.5 µg ml −1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

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Determination of gentamicin in bovine milk using liquid chromatography with post-column derivatization and fluorescence detection

Cited by 34 publications

References 12 publications

Confirmation of gentamicin and neomycin in milk by weak cation-exchange extraction and electrospray ionization/ion trap tandem mass spectrometry

Confirmation of gentamicin and neomycin in milk by weak cation-exchange extraction and electrospray ionization/ion trap tandem mass spectrometry

HPLC determination of colistin and aminoglycoside antibiotics in feeds by post-column derivatization and fluorescence detection

Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

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