Determination of genotoxic effects of chlorfenvinphos and fenbuconazole in Allium cepa root cells by mitotic activity, chromosome aberration, DNA content, and comet assay

Türkoğlu, Şifa

doi:10.1016/j.pestbp.2012.06.001

Cited by 73 publications

(35 citation statements)

References 40 publications

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“…Anilofos contains a P=S bond; therefore, in our opinion, this compound may be cytotoxic due to its chemical structure and biological activity in the cell. There are several supporting data about OPs in previous studies parallel to our study (Arañez and Rubio 1993;Lamsal et al 2010;Türkoglu 2012). …”

Section: Discussionsupporting

confidence: 92%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionsupporting

confidence: 92%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…Depression of mitotic indices and root growth inhibition in A. cepa have been reported earlier following the exposure to a number of pollutants including heavy metals (Barbosa et al, 2010;Hemachandra & Pathiratne, 2015), pesticides (Liman et al, 2011;Türkoğlu, 2012), complex mixtures such as industrial and municipal waste (Chandra et al, 2005;Radic et al, 2010;Siddiqui et al, 2011) and emerging contaminants (Herrero et al, 2012). Allium cepa root tip cells exposed to undiluted and diluted wastewater /downstream water samples of the Dandugan Oya exhibited significant depression of mitotic indices in comparison to the dilution water in all sampling occasions.…”

Section: Discussionsupporting

confidence: 63%

“…Moreover, this test system provides information to evaluate the genotoxic mechanisms of an agent (clastogenic and/or aneugenic effects) on the genetic material (Leme & Marin-Morales, 2009). Recently, cytotoxic and genotoxic effects on A. cepa have been reported following exposure to a number of pollutants including heavy metals (Barbosa et al, 2010;Hemachandra & Pathiratne, 2015), pesticides (Liman et al, 2011;Türkoğlu, 2012), complex mixtures such as industrial and municipal wastes (Chandra et al, 2005;Radic et al, 2010;Siddiqui et al, 2011) and emerging contaminants (Herrero et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Toxicity assessment of industrial wastewaters reaching Dandugan Oya, Sri Lanka using a plant based bioassay

Kannangara¹,

Pathiratne²

2015

J. Natn. Sci. Foundation Sri Lanka

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Industrial waste may contain complex chemical mixtures with potential cytotoxic and genotoxic effects. The Dandugan Oya, a water canal located in the Western Province of Sri Lanka receives industrial waste from multiple sources. In the present study potential toxicity of selected industrial wastewaters reaching the Dandugan Oya, and the downstream water was assessed using a plant based bioassay with onion (Allium cepa L. var. ascalonicum) as the test organism. Of the physico-chemical characteristics tested, temperature, pH, biochemical oxygen demand, chemical oxygen demand, cadmium and chromium levels of the wastewaters collected during three sampling occasions in the year 2012 were within the national tolerance limits specified for the discharge of industrial effluents into inland surface waters. The exposure of A. cepa bulbs to wastewater and downstream water from the Dandugan Oya resulted in the reduction of root growth (24 -62 %) and mitosis (31 -55 %), induction of micronuclei (up to 0.6 %), nuclear abnormalities (3 -14 folds) and chromosomal aberrations (3 -21 folds) in the root tip meristematic cells compared to those exposed to the control and the upstream water, indicating cytotoxic and genotoxic effects. No significant difference between the control and the upstream water was found in relation to the measured biological effects (p > 0.05). The present study revealed that the tested wastewaters contained cyto-genotoxic contaminants and, the inherent dilution/detoxification capacity of Dandugan Oya during the study period was not adequate to eliminate the toxic effects in the downstream water. In addition to the conventional physico-chemical analyses, inclusion of suitable bioassays as additional assessments in water quality monitoring programmes could alert cyto-genotoxic impacts in wastewater receiving inland surface waters.

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“…In another study, genotoxic effects of chlorfenvinphos and fenbuconazole were used for comet assay on the root meristem cells of A. cepa. Increasing concentrations were used on the roots for 24 and 48 h and DNA damage was demonstrated in treatments chemical groups compared to the control 38 . In this study, after the exposure period, single strand DNA breaks by comet assay were used.…”

Section: Discussionmentioning

confidence: 99%