Determination of genomic instability and DNA methylation effects of Cr on maize (Zea mays L.) using RAPD and CRED-RA analysis

Erturk, Fılız Aygun; Ağar, Güleray; Arslan, Esra; Nardemir, Gokce; Sahin, Zehra

doi:10.1007/s11738-014-1529-5

Cited by 25 publications

(23 citation statements)

References 40 publications

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“…The value of GTS was determined for each glyphosate treated sample. The GTS value of the control was set to 100 %, and treated samples were calculated based on the value of the control (Erturk et al 2014). CRED-RA test results were calculated with the following formula:…”

Section: Agarose Gel Electrophoresismentioning

confidence: 99%

Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Nardemir

Ağar

Arslan

et al. 2015

Theor. Exp. Plant Physiol.

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In this study, the most commonly used herbicide, glyphosate, was investigated for its genotoxic effects on the genome of Triticum aestivum. Five different concentrations of the herbicide were used, and alterations to DNA were measured quantitatively based on their RAPD (Randomly Amplified Polymorphic DNA) profiles. The genomic template stability (GTS%) at each concentration was evaluated, and a decrease was observed with increasing glyphosate concentration. Thus the highest concentration was concluded to be the most effective for causing alteration to DNA. Additionally, the coupled restriction enzyme digestion-random amplification (CRED-RA) technique was used to determine an epigenetic mechanism, e.g. DNA methylation. The polymorphic percentages of all concentration were calculated after herbicide applications. When in glyphosate doses compared with control group, all applications of glyphosate observed to consist of methylation. The methylation levels range from 28.3 to 73.9 % (DNA hypermethylation). In conclusion, based on the RAPD and CRED-RA results, glyphosate causes DNA alterations and methylation.

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Section: Agarose Gel Electrophoresismentioning

confidence: 99%

Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Nardemir

Ağar

Arslan

et al. 2015

Theor. Exp. Plant Physiol.

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“…On the other hand, germination tests performed with isogenic seeds of several higher plants and comparative RAPD profile analysis with known primers of genomic DNA samples from the germinated seedlings may be used for determining toxicological properties of test materials on eukaryotic systems, which have more complex metabolic pathways than prokaryotic cells (Aslan et al, 2012; Dhakshanamoorthy et al, 2011; Erturk et al, 2014; Song et al, 2013; Taspinar et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic evaluation of newly synthesized iminothiazolidinones

et al. 2017

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The current study was designed to assess the potential toxicological effects of newly synthesized iminothiazolidinones by employing Ames Salmonella, Escherichia coli WP2, Zea mays seed germination, and random amplified polymorphic DNA (RAPD) assay systems. The bacterial tester strains S. typhimurium TA1535, TA1537, TA1538, TA98, TA100, and E. coli WP2 uvrA were chosen to test the direct gene mutation inducing capabilities of the test materials in prokaryotic systems and Z. mays seeds for determination of potential toxicological effects in eukaryotic systems. OPA-3 and OPA-6 primers were used in the RAPD analysis to determine genotoxic activities on the eukaryotic genomes. According to the results, none of the test materials showed significant mutagenic activity on the bacterial tester strains at the chosen concentrations. Additionally, none of the tested compounds showed inhibition of the germination of Z. mays seeds. In contrast, the RAPD analysis results were inconsistent with the bacterial reversion assays and the seed germination assay results. All test materials significantly changed the RAPD profiles for OPA-3; however, only compound 5 showed a significant change for OPA-6 when compared with the control groups. In conclusion, the newly synthesized iminothiazolidinone derivatives (C1-C5) were determined as potentially genotoxic compounds and they should be checked with multiple toxicology test systems before further studies to determine their actual use.

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“…Genomic DNA was extracted using the procedure of Erturk et al (2014). Approximately 10-15 mg of fresh sample was powdered in liquid nitrogen and added to a 2 mL tubes.…”

Section: Genomic Dna Isolationmentioning

confidence: 99%

Cytogenetic and epigenetic alterations by cobalt and nickel onZea maysL

Erturk

Ağar

Nardemir

et al. 2015

Toxicological & Environmental Chemistry

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Changes in DNA methylation in Zea mays plantlets in response to cobalt and nickel (5, 10, 20 and 40 mmol L -1 ) evaluated by the coupled restriction enzyme digestion-random amplification technique revealed dose-related increases in hypermethylation. The effects on mitotic index and phytohormone levels were also determined. Co and Ni caused a decrease in mitotic index and an attenuation in the growth-promoting hormones gibberellic acid, zeatin and indole acetic acid, while abscisic acid levels rose. These findings have shown that the increase in abscisic acid levels and DNA methylation depend on the concentrations of Co and Ni.

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Determination of genomic instability and DNA methylation effects of Cr on maize (Zea mays L.) using RAPD and CRED-RA analysis

Cited by 25 publications

References 40 publications

Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Genotoxic evaluation of newly synthesized iminothiazolidinones

Cytogenetic and epigenetic alterations by cobalt and nickel onZea maysL

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