Determination of genetic diversity in indigenous cultivars of onion (<i>Allium cepa L.</i>) by multivariate analysis under mid hill zone of North-Western Himalayas, India

Singh, Yudhvir; Vishalakshi,; Sekhon, Bhallan Singh; Verma, Aseem; Sharma, Surbhi; Sharma, Simran

doi:10.5958/0975-928x.2019.00027.9

Cited by 3 publications

(5 citation statements)

References 7 publications

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“…This genetic diversity among the genotypes may have been due to the restricted gene ow following geographic barriers, or exhaustive natural and human selection which resulted in the formation of various single clusters. Different patterns in onion genotypes were also reported by earlier workers [36,62,3,45,16,47,2]. The highest inter-cluster distance (364.35) was exhibited between the clusters V and VII, indicating that these genetically diverse clusters can provide relatively better parental lines that can be utilized in future breeding programmes for obtaining a wide spectrum of variation.…”

Section: Discussionsupporting

confidence: 64%

“…Hence, these genotypes can be utilized for mapping studies for stemphylium blight and thrips infestation in onion genotypes. Earlier workers [16,47,30] also found that parents from diverse clusters may be utilized in hybridization programme to obtain desirable transgressive segregants. The minimum inter-cluster distance was found between clusters I and VI (75.06), suggesting that the genotypes in these clusters were closely related.…”

Section: Discussionmentioning

confidence: 99%

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Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Kapoor

Sood

Jayaswall

et al. 2023

Preprint

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Background It is crucial to gain understanding of crop diversity at the genetic and phenotypic levels. For breeding onion with higher yield and quality together with greater resilience to biotic and abiotic stressors agro-morphological and molecular characterization of onion genotypes is of utmost importance. Methods and results In the present study, genetic diversity in 49 onion genotypes were assessed using 6 agro-morphological descriptors, 19 quantitative traits along with 23 ISSR markers. All the agro-morphological descriptors were found polymorphic with Bulb: basic colour of dry skin (1.44) exhibiting the highest diversity index. The multivariate analysis using Mahalanobis D2 statistic grouped 49 genotypes into seven clusters with highest inter cluster distance between V and VII (364.35). A total of 78 fragments were produced from 13 polymorphic primers with a mean of 6 alleles per primer. The polymorphic information content (PIC) ranged from 0.42 (UBC 835) to 0.75 (UBC 825) with a mean of 0.61 per primer. With a mean of 0.36, the inter-genotype genetic distance ranged from 0.12 to 0.72. Based on cluster analysis using UPGMA algorithm, the genotypes were divided into two major clusters, whereas the cluster tree constructed using the described ISSR markers identified three major groups. The structure analysis divided the population into two main groups. Conclusion From the findings of present study, it can be stated that characterization at both molecular and morphological basis is of utmost importance to understand the genetic diversity in onion. Hybridization between distantly related genotypes can produce desirable transgressive segregants in future onion breeding programmes.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Kapoor

Sood

Jayaswall

et al. 2023

Preprint

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“…In the present investigation, morphological characters such as germination percentage, leaf colour, plant height, leaf waxiness, bulk shape, bulk colour and leaf foliage attitude were studied to differentiate genotypes. Similarly, (Singh et al, 2013) [14] concluded that the genotypes evaluated the traits differ clearly from each other and form a very reliable morphological descriptive profile of onion genotypes. Among 4 RAPD primers, three primers (OPA-16, OPB-16, and OPA-9) showed 100% polymorphism and only OPB-9 produced 92.85% polymorphism.…”

Section: Discussionmentioning

confidence: 94%

“…The primers OPB-09, OPA-16, OPB-16 and OPA-09 were found to be the most effective in generating unique bands. The highest number of bands (14) were recorded with primer OPB-09 and while the least number of bands (09) were observed by OPA-09. The OPB-16 and OPA-16 showed 12 and 10 bands respectively.…”

Section: Genetic Diversity Analysis By Using Rapd Markersmentioning

confidence: 93%

Morphological and molecular characterization of onion (Allium cepa L.) genotypes using RAPD markers

Mane-Deshmukh¹,

Ghodake²,

Kharpude³

et al. 2023

Pharma Innovation

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The present study carried out to with a view to characterize and validate the RAPD marker's genetic diversity at morphological and molecular levels in thirty cultivars of onion (Allium cepa L.). This work aimed to determine their important traits, DNA polymorphism and decipher their genetic variation. The agro-morphological characters such as germination percentage, plant height, leaf colour, leaf waxiness, leaf foliage attitude, bulk colure and bulk shape of thirty onion genotypes were studied. The genetic diversity assessed using four RAPD markers (OPB-09, OPA-16, OPB-16, and OPA-09). It revealed significant genotypic differences in the onion genotypes. Significant genetic diversity was observed and revealed by UPGMA analysis. The RAPD analysis yielded 98.21% average polymorphism (genetic diversity) with 901 amplicons. The OPA-16, OPB-16, and OPA-9 showed 100% polymorphism and only OPB-9 produced 92.85% polymorphism. The RAPD primers discriminated thirty cultivars into two major clusters by the method of UPGMA. The major cluster (cluster-A) contains 28 genotypes and another was a minor cluster (cluster-B) which contains only two genotypes (1403 and 1432/33). The cultivars exhibited statistically significant variability, which gives an opportunity to use them for varietal development through breeding programs. The key findings of the current study would be an insight for breeding strategies in onion cultivars.

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“…Para uma mesma combinação de genótipos observou-se tendência de aumento na distância genética com o intercruzamento de plantas na maior parte dos casos (Tabela 1). Wanser et al, (2012) estimando a variabilidade genética de genótipos de cebola em Santa Catarina também observaram diferenças na divergência entre os genótipos com relação ao local de plantio, Singh et al, (2013) constatou divergência significativa entre os mesmos genótipos de cebola submetidos à condições de dia curto e longo.…”

Section: Resultsunclassified

Fenotipagem e genotipagem de populações de cebola com diferentes níveis de endogamia

Machado¹

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Dedico esta tese a Deus. Criador de tudo que existe. Sinônimo de toda forma de amor. Aos meus pais Esio Euclides Machado e Liliam Fernandes de Lima Machado E a meu avô Osvaldo Francisco da Abadia in memoriam AGRADECIMENTOS Agradeço em primeiro lugar a Deus, por ter me dado tudo e o que foi necessário para o cumprimento desta fase da minha vida. A inteligência, a saúde, a persistência, e as pessoas que me ajudaram nesta caminhada. À minha amada família, em especial meus pais Esio e Liliam e meus irmãos Rafael, Ana Cláudia, João Paulo e Júlia, por tudo que fizeram por mim e pelo amor incondicional. Pelo carinho, apoio e confiança de que eu iria chegar ao final de mais esta fase. Com uma família como vocês, com pais, irmãos, tios, primos e avós excepcionais, a jornada com certeza foi mais alegre e fácil. À minha noiva Letícia, que sempre esteve próximo apoiando e incentivando, com conselhos e orações. Uma ótima companheira e amiga para toda a vida, te amo! Ao meu orientador Maguinho, pelos ensinamentos, pelo tempo despendido em minha orientação e pelas conversas informais. Com certeza levarei deste tempo muito mais que apenas a tese. Ao melhorista de cebola da Bayer Joelson André de Freitas por todos os ensinamentos, momentos de conversa e amizade, exemplo de profissional a ser seguido por mim, agradeço pela confiança, você foi essencial para realização deste trabalho. À Bayer CropScience Vegetable Seeds, na figura do seu diretor de pesquisa Ailton Ribeiro, pela oportunidade de realização deste trabalho e vivência em uma empresa de excelência em pesquisa. Agradeço de coração a todos os demais melhoristas, funcionários de escritório e de campo. Aos meus coorientadores e professores Ana Paula Oliveira Nogueira e Gabriel Maciel Mascarenhas, pela contribuição na melhoria deste trabalho. A Roberta Camargos de Oliveira, por ter contribuído na melhoria deste trabalho e pelos ensinamentos durante a defesa da tese. Agradezco mis tutoras de tesis en España, Cristina Mallor y Aurora Díaz, por la confianza de tenerme en su laboratorio en el Centro de Investigación Agroalimentaria de Aragón (CITA), realizando parte de esta investigación. Soy inmensamente agradecido y siempre recordaré a todos en Zaragoza. À toda equipe do LAGEN (Laboratório de Análise de Sementes e Recursos Genéticos) pelo apoio nas análises moleculares RAPD, agradeço ao professor Gabriel Maciel Mascarenhas, professora Ana Carolina Silva Siquieroli e ao técnico Igor Forigo. Ao pessoal do Laboratório de Bioquímica e Biologia Molecular (LABIBI), cito aqui a técnica Mariana e a professora Ana Maria Bonetti. À Universidade Federal de Uberlândia pela oportunidade de cursar o doutorado em Produção Vegetal.À CAPES pela concessão da bolsa de estudos, que a pesquisa não seja vista como um gasto e sim como um investimento.À FAPEMIG pelo auxílio financeiro.À todos os professores que tive em minha vida ofereço esta tese, minhas conquistas e caráter passa por vocês. Agradeço pela luta diária e pela motivação não monetária.

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Determination of genetic diversity in indigenous cultivars of onion (Allium cepa L.) by multivariate analysis under mid hill zone of North-Western Himalayas, India

Cited by 3 publications

References 7 publications

Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Morphological and molecular characterization of onion (Allium cepa L.) genotypes using RAPD markers

Fenotipagem e genotipagem de populações de cebola com diferentes níveis de endogamia

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