Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
Background. Various trace evidence are often found on the instruments of mechanical injury, and among them are microparticles of humans damaged organs and tissues. The detection of cells and microparticles of organs and tissues on the instrument of injury confirms the fact of its contact with the human body, which is of great evidentiary value. Case presentation. The article presents the results of forensic medical cytological examination of biological evidence taken from the knifes blade in the case of multiple penetrating stab/cut wounds of the chest with а lung damage. Microscopic preparations were prepared from the traces on knifes blade, which were stained with solutions of fluorochromes (0.0005 % aqueous solution of atebrine and 0.01 % solution of acridine orange). The study was performed using the luminescent microscope "LEICA DM 2500" (объективы 20х и 60х, окуляр 10х). Within the research the microparticles of striated skeletal muscle, areolar connective tissue and fat tissue with fragments of capillaries and arterioles were detected. The isolated mesothelial cells and type II pneumonocytes were also detected. The fibers of striated skeletal muscle tissue looked like long multinucleated cells with distinguishing cross-striation. Areolar connective tissue was represented by loosely arranged fibers which were embedded in the ground substance. The fragments of fat tissue looked like the "clusters" of round and oval large cells, tightly pressed together, with capillaries and arterioles between. Y-chromatin was detected in the nuclei of most cells. In addition, human blood was found on the knifes blade. Conclusion. Thus, as a result of the study, tiny particles of tissues and isolated human cells of the male genetic sex were detected, their organ-tissue affiliation was established, which is a valuable diagnostic find, since it confirms the fact of contact of the trauma instrument with the human body.
Background. Various trace evidence are often found on the instruments of mechanical injury, and among them are microparticles of humans damaged organs and tissues. The detection of cells and microparticles of organs and tissues on the instrument of injury confirms the fact of its contact with the human body, which is of great evidentiary value. Case presentation. The article presents the results of forensic medical cytological examination of biological evidence taken from the knifes blade in the case of multiple penetrating stab/cut wounds of the chest with а lung damage. Microscopic preparations were prepared from the traces on knifes blade, which were stained with solutions of fluorochromes (0.0005 % aqueous solution of atebrine and 0.01 % solution of acridine orange). The study was performed using the luminescent microscope "LEICA DM 2500" (объективы 20х и 60х, окуляр 10х). Within the research the microparticles of striated skeletal muscle, areolar connective tissue and fat tissue with fragments of capillaries and arterioles were detected. The isolated mesothelial cells and type II pneumonocytes were also detected. The fibers of striated skeletal muscle tissue looked like long multinucleated cells with distinguishing cross-striation. Areolar connective tissue was represented by loosely arranged fibers which were embedded in the ground substance. The fragments of fat tissue looked like the "clusters" of round and oval large cells, tightly pressed together, with capillaries and arterioles between. Y-chromatin was detected in the nuclei of most cells. In addition, human blood was found on the knifes blade. Conclusion. Thus, as a result of the study, tiny particles of tissues and isolated human cells of the male genetic sex were detected, their organ-tissue affiliation was established, which is a valuable diagnostic find, since it confirms the fact of contact of the trauma instrument with the human body.
The article presents a case of poisoning with lambda-cyhalothrin, a synthetic pyrethroid, in a family of three (2 deaths at different times of the course of poisoning). A case from our practice is of interest not only from the point of view of the frequency of occurrence of this type of poisoning, but also as an example of a complex diagnostic process, including an analysis of the circumstances, clinical data and a set of post-mortem studies, taking into account the detection of a toxicant that has the ability to break down in minimal quantities in the food samples from the scene. Data indicating polymorphism of manifestations of poisoning with synthetic pyrethroids is given. A post-mortem study revealed biochemical and micromorphological markers of this poisoning diagnosed with acute toxic hepatitis, lesions of the gastrointestinal tract, resulted in hepatic and renal failure with laboratory confirmation, excretory and necrotic nephrosis with severe hemocirculatory disorders in internal organs and tissues. Additionally, an experimental model was created on laboratory animals (rats) to confirm the possibility of developing these lesions during the course of poisoning with a synthetic pyrethroid, while in the experimental model not only a similar clinical picture was obtained, but also the same results of laboratory studies. The presented data of foreign studies, a brief epidemiological report, as well as a detailed description of the comprehensive post-mortem diagnosis based on clinical and morphological, toxicology, biochemical, microscopic and experimental data show that in the study of such cases, an individual approach is required for the most accurate answers to the questions and prevention of these poisonings.
Background: Sex determination is essential in forensic science, anthropology, and medical diagnostics. Traditional methods, such as DNA analysis, are resource intensive and time-consuming. This pilot study explores the utility of oral rinse-based cytology for detecting Barr bodies in exfoliated oral mucosal cells from saliva as a more accessible and efficient alternative. Aim: This study aimed to assess the viability of rinse-based cytology in identifying sex through the detection of Barr bodies. Materials and Methods: In the study, 60 participants aged 20–30, free of lesions, and nonsmokers/nondrinkers, were evenly divided into male and female groups. Unstimulated saliva was collected from each participant between 9 and 10 am Participants were instructed to tilt their heads slightly forward to accumulate saliva and then spit into disposable sterile containers. After centrifugation, two smears were prepared and stained with Papanicolaou (PAP) and hematoxylin and eosin (H and E) stains. The smears were labeled (single-blinded) and examined for the presence of Barr body by two oral pathologists. Results: H and E stain had a mean Barr body count of 2.38 and a standard deviation of approximately 2.731, whereas PAP stain had a higher mean count of 3.57 with a standard deviation of 3.933. Levene’s test showed significant differences (P = 0.000) in variances between the staining methods (F = 21.001, df = 118, P < 0.001), but the average Barr body count difference of about 1.183 with P = 0.058 was not statistically significant (P > 0.05). Conclusion: Oral rinse-based cytology offers a noninvasive, cost-effective, and time-efficient approach to sex determination. While this pilot study demonstrates its potential, further research involving larger and more diverse populations is required to validate its reliability fully. This innovative approach has the potential to impact various scientific and forensic disciplines, offering a practical alternative to traditional methods.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.