Determination of gambogic acid in human plasma by liquid chromatography-atmospheric pressure chemical ionization–mass spectrometry

Ding, Li; Huang, Di; Wang, Jinwan; Li, Shuting

doi:10.1016/j.jchromb.2006.08.034

Cited by 14 publications

(14 citation statements)

References 17 publications

(18 reference statements)

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“…Following an overnight fast, each volunteer received a single intravenous dose of 45 mg/m 2 of the GA injection. The intravenous infusion of the GA injection was designated to finish within 2 h. Blood was sampled pre-dose and at 1, 2, 2.5, 3, 4, 6, 9, 12, 24, 36, and 48 h following dosing for determination of plasma concentrations of GA [18] and 10-OHGA. Model-independent pharmacokinetic parameters were calculated for 10-OHGA.…”

Section: Applicationmentioning

confidence: 99%

“…At first, mass spectrometric condition selection was conducted by changing the fragmentor voltage. Over the range of 50-140 V, the base peak in the mass spectra of 10-OHGA obtained was the depro- Due to the selected target ion of 10-OHGA at m/z 627.3 was also the deprotonated molecule ion of GA [18], GA and 10-OHGA needed to be well separated [25]. In the method development, GA was, therefore, spiked into the human blank plasma as well and chromatographic conditions were carefully optimized.…”

Section: Optimization Of Lc-esi-ms For Quantitative Determination Of mentioning

confidence: 99%

“…Several HPLC-UV methods have been described for quantification of GA in rat plasma and tissues [16] and in dog plasma [17]. Previously, we have reported an LC-APCI-MS method for quantification of GA in human plasma and it has been successfully applied for the clinical pharmacokinetic study [18]. However, the quantitative method for the determination of GA metabolite in bio-fluid has not been reported up to now.…”

Section: Introductionmentioning

confidence: 99%

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Identification and quantitative determination of a major circulating metabolite of gambogic acid in human

Yang

Ding

Shao-hong

et al. 2010

Journal of Chromatography B

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Section: Applicationmentioning

confidence: 99%

Section: Optimization Of Lc-esi-ms For Quantitative Determination Of mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and quantitative determination of a major circulating metabolite of gambogic acid in human

Yang

Ding

Shao-hong

et al. 2010

Journal of Chromatography B

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“…Growth inhibition induces the G0/G1 phase cell cycle arrest, and apoptosis could be caused by gambogic acid in human chronic myelogenous leukemia cell line K562 cells. Moreover, 11 Garcinolic gambogic acid might show its strong antitumor effects through the interruption of SRC-3 (68). Gambogic acid showed both growth arrest and apoptosis induction effects on Jurkat cells by significantly suppressing the expression of nucleophosmin, which indicated the fact that nucleophosmin and some nucleoporins might serve as new targets of gambogic acid (69).…”

Section: Inhibition Of Proliferation and Induction Of Apoptosisrelatementioning

confidence: 99%

Recent Research on Bioactive Xanthones from Natural Medicine: Garcinia hanburyi

Jia

et al. 2015

AAPS PharmSciTech

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Abstract. Garcinia hanburyi, a tropical plant found in south Asia, has a special long history in the development of both medicine and art. This review mainly focuses on the pharmacy research of the bioactive compounds from the plant in recent years. Preparative and analysis separation methods were introduced. Moreover, the chemical structure of the isolated compounds was included. The studies of biological activities of the caged xanthones from the plant, including antitumor, anti-HIV-1, antibacterial, and neurotrophic activities, were reviewed in detail. Furthermore, the mechanisms of its antitumor activity were also reviewed. As mentioned above, some of the xanthones from G. hanburyi can be promising drug candidates, which is worth studying. However, we still need much evidence to prove their efficacy and safety. So, further research is critical for the future application of xanthones from G. hanburyi.

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“…Several HPLC methods have been developed to determine gambogic acid from gamboges Yang et al, 1999;Zhang et al, 2003) and the biological matrices of human (Ding et al, 2007) and animals (Hao et al, 2007). Interestingly, an HPLC and an HPCE method were established to identify natural dyes, including gambogic acid, in historical drawings and maps (Blanc et al, 2006;Lopez-Montes et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Improved high‐performance liquid chromatographic method for simultaneous determination of 12 cytotoxic caged xanthones in gamboges, a potential anticancer resin from Garcinia hanburyi

Song

Han

et al. 2008

Biomedical Chromatography

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The potential anti-tumor activity of gamboges, a herbal medicine derived from Garcinia hanburyi, has increasingly gained the interest of scientist worldwide. The major components of gamboges are cytotoxic caged xanthones. In the present study, an improved HPLC method was developed to simultaneously quantify 12 caged xanthones, including three pairs of epimers and four pairs of trans-cis isomers, i.e. forbesione, isomorellic acid, morellic acid, R-30-hydroxygambogic acid, S-30-hydroxygambogic acid, isogambogenic acid, gambogenic acid, gambogellic acid, R-isogambogic acid, S-isogambogic acid, R-gambogic acid and S-gambogic acid. This method was validated to be sensitive, precise and accurate with limits of detection of 0.03-0.08 microg/mL, overall intra-day and inter-day variations less than 7.9% and overall recovery over 93.2%. The correlation coefficients (r(2)) of the calibration curves were higher than 0.995 for all analytes. The newly established method was successfully applied to reveal the difference in the chemical profiles and contents of these analytes in gamboges from different origins. It can be concluded that this method was not only an effective quality control method to ensure the safety and efficacy consistency of gamboges, but also a useful tool for screening and determining more potent cytotoxic xanthones with potential anticancer activity.

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Determination of gambogic acid in human plasma by liquid chromatography-atmospheric pressure chemical ionization–mass spectrometry

Cited by 14 publications

References 17 publications

Identification and quantitative determination of a major circulating metabolite of gambogic acid in human

Identification and quantitative determination of a major circulating metabolite of gambogic acid in human

Recent Research on Bioactive Xanthones from Natural Medicine: Garcinia hanburyi

Improved high‐performance liquid chromatographic method for simultaneous determination of 12 cytotoxic caged xanthones in gamboges, a potential anticancer resin from Garcinia hanburyi

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