Determination of Gabapentin in Serum Using Solid-Phase Extraction and Gas-Liquid Chromatography

Abstract

A gas-liquid chromatographic method for the determination of gabapentin (Neurontin) is described. The method involves extracting 0.5 mL of acidified sample by C18 solid-phase column, derivatization with MTBSTFA plus 1% tBDMCS, and analysis on an HP-1 column with a flame-ionization detector. Quantitation was performed with peak-height ratios of gabapentin to a gabapentin analogue [(1-aminomethyl-1-cycloheptyl) acetic acid] as the internal standard. The assay had a limit of detection of 0.2 mg/L and a linear range from 0.5 to 30.0 mg/L. Several compounds were analyzed for potential interference, and none interfered with the assay.

Simultaneous determination of three anticon‐vulsants using hydrophilic interaction LC‐MS

“…Gas chromatographic methods require derivatization. GC with flame ionization detector is used for the determination of gabapentin [11] and GC/MS methods are reported for the determination of gabapentin and vigabatrin [12,13]. Numerous HPLC methods have been published about the determination of gabapentin, pregabalin or vigabatrin in biological fluids using different derivatizing reagents [14 -16].…”

Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring

“…The method most commonly used for GBP quantification in blood or serum is reversed-phase HPLC with derivatization and fluorometric detection [146][147][148]. GC methods have also been reported [149], and more recently, more sensitive and validated LC procedures with MS or MS/MS methods for both clinical and experimental monitoring of GBP in human and animal have been also been described [149][150][151][152][153].…”

Procedure for the Monitoring of Gabapentin with 2,4,6-Trinitrobenzene Sulfonic Acid Derivatization Followed by HPLC with Ultraviolet Detection

“…Several types of analysis have been performed, including spectrofluorimetry (15)(16)(17) and HPLC with a variety of detection methods, including mass spectrometry and capillary electrophoresis, but most frequently, fluorescent derivatization (18 -23 ). Gas chromatography (GC) has also been used with detection by mass spectrometry (MS) and flame ionization (24,25 ). Here we describe a rapid and cost-effective isocratic liquid chromatography method that uses commonly available ultraviolet detection for gabapentin quantification without the need for solid-phase extraction.…”