Procedure for the Monitoring of Gabapentin with 2,4,6-Trinitrobenzene Sulfonic Acid Derivatization Followed by HPLC with Ultraviolet Detection

Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study

“…Derivatization by chromophoric reagents increases the sensitivity of gabapentin detection. Different derivatizing reagents have been used before for determination of gabapentin [5][6][7][8][9][10][11][12][13][14][15][16][17].…”

“…Gabapentin has no significant ultraviolet, visible or fluorescence absorption. Several HPLC methods have been published for determination of gabapentin in human plasma using different derivatizing reagents such as 2,4,6-trinitrobenzene sulphonic acid [5,6], phenylisothiocyanate (PITC) [7], O-phthaldialdehyde (OPA) [8][9][10][11][12][13][14], 4-chloro-7-nitrobenzofurazan [15], 9-fluorenylmethylchloroformate [16] and 1, 2-naphthoquinone-4-sulphonic acid sodium salt [17].…”

Therapeutic Drug Monitoring of the Newer Anti-Epilepsy Medications

“…An approximate reference range of 2–20 mg/L has been proposed [44]. Multiple analytical methodologies have been reported for the measurement of gabapentin in plasma/serum including HPLC [68,69], HPLC-tandem mass spectrometry (LC/MS/MS) [70], gas chromatography/mass spectrometry (GC/MS) [71] and GC/MS/MS [72]. …”

Rapid and validated fluorometric HPLC method for determination of gabapentin in human plasma and urine for clinical application

“…The analytical method is much shorter and simpler than other reports which use other derivatization reagents. [6][7][8][9][10][11][12][13][14][15][16][17][18] Derivatization of gabapentin and baclofen using NBD-F and their HPLC separation required 1AE0 and 3AE5 min, respectively. This precolumn derivatization method without sample clean-up can be easily applied to the preparation of a great number of samples using 96-well thin wall microplates.…”

“…Several analytical methods have been developed for the determination of gabapentin in human plasma. Gabapentin concentration in plasma has been measured using several derivatization reagents such as 4-chloro-7-nitrobenzofurazan, 6 1-fluoro-2,4-dinitrobenzene, 7 2,4,6-trinitrobenzene sulfonic acid, 8,9 1,2-naphthoquinone-4-sulphonic acid sodium salt, 10 (2-naphthoxy)acetyl chloride, 11 and o-phthaladehyde (OPA), [12][13][14][15][16][17][18] because gabapentin has no specific visible and ultraviolet absorption or fluorescence. Most of the methods employed OPA for the derivatization.…”