Determination of Free Amino Acids by Titration of the Carbon Dioxide Formed in the Reaction With Ninhydrin

Slyke, Donald D. Van; MacFadyen, Douglas A.; Hamilton, Paul B.

doi:10.1016/s0021-9258(18)72811-1

Cited by 183 publications

(9 citation statements)

References 5 publications

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“…The formation of DNP-glutamic acid upon treatment of linatine with Sanger's reagent, followed by hydrolysis, indicated a free amino group on the glutamic acid portion of the linatine molecule. The evolution of approximately 1 mole of carbon dioxide upon treatment with ninhydrin according to the method of Van Slyke et al (1941) suggested that the -carboxyl of the glutamyl residue was free and that the linkage was formed through the -carboxyl of glutamic acid.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*

Klosterman¹,

Lamoureux²,

Parsons³

1967

Biochemistry

109

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Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*

Klosterman¹,

Lamoureux²,

Parsons³

1967

Biochemistry

109

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“…Phosphate was estimated colorimetrically by the method of Fiske & SubbaRow (1925). Total free amino acids were estimated by the method of Van Slyke, MacFadyen & Hamilton (1941). In this method the carbon dioxide evolved on decarboxylation with ninhydrin, indane-trione hydrate, is absorbed by barium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

The Inorganic and Amino Acid Composition of Some Lamellibranch Muscles

Potts

1958

Journal of Experimental Biology

134

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1. Measurements have been made of the inorganic ion and free amino acid content of a number of lamellibranch muscles. The volumes of extracellular fluid in the muscles have also been determined so that the intracellular concentrations can be calculated. 2. The fast portion of the adductor muscle of Pecten contains about 160 mM. K/kg. fibre water and only 43 mM. Na/kg. fibre water and 34 mM. Cl/kg. fibre water. The potassium and chloride are approximately in a Donnan equilibrium with the potassium and chloride in the blood. 3. In the slow portion of the adductor muscle of Pecten and in the two parts of the adductor and in the byssus retractor of Mytilus, the concentrations of potassium in the fibres are from 150 to 160 mM./kg fibre water, of sodium 73 to 95 mM./kg. fibre water and chloride 94 to 152 mM./kg. fibre water. The potassium and chloride in the fibres are not in a Donnan equilibrium with the potassium and chloride in the blood. 4. The fast and slow fibres of the adductor muscles of Anodonta contain 21 and 17 mM./kg. fibre water of potassium respectively, 5·3 and 7·2 mM./kg. fibre water of sodium, and 2·4 and 5·4 mM./kg. fibre water of chloride. The potassium and chloride in the fibres is not in a Donnan equilibrium with the potassium and chloride in the blood. 5. The fast fibres of Mytilus adductor contain 295 mM./kg. fibre water of free amino acids and 39 mM./kg. fibre water of acid-soluble phosphate compounds. The fast fibres of Anodonta adductor contain only 11 mM./kg. fibre water of amino acids and 19·8 mM./kg. fibre water of phosphate compounds. 6. Mytilus muscles fibres adapt to a reduced blood concentration, partly by an increase in water content and partly by a reduction in the sodium, chloride and free amino acid content. 7. Anodonta muscle fibres adapt to an increased blood concentration, partly by a reduction in the water content and partly by an increased sodium and chloride content. 8. The significance of these results is discussed. It is concluded that the ionic contents of the lamellibranch smooth muscles are consistent with equilibria systems in which the permeability to sodium is significant compared with the permeability to potassium and in which both a sodium and a potassium pump operate.

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“…This compound gave a yellow color with ninhydrin and the paper chromatogram showed a yellow spot, Rf = 0.64. Heating of -aminocrotonic acid with a ninhydrin solution does not result in the evolution of carbon dioxide, under the conditions developed by Van Slyke and co-workers (8) for the determination of free -amino acids by the ninhydrincarbon dioxide reaction. Hydrogenation of -aminocrotonic acid over platinum oxide catalyst required one mole of hydrogen and yielded -aminobutyric acid.…”

Section: Rch(nh2)ch=chc02hmentioning

confidence: 99%

CONTRIBUTION TO THE KNOWLEDGE OF Γ-Aminocrotonic ACID. VINYLOGS OF Α-Amino ACIDS. I

Balenović¹,

Jambresic²,

Urbas³

1954

J. Org. Chem.

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Determination of Free Amino Acids by Titration of the Carbon Dioxide Formed in the Reaction With Ninhydrin

Cited by 183 publications

References 5 publications

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*

The Inorganic and Amino Acid Composition of Some Lamellibranch Muscles

CONTRIBUTION TO THE KNOWLEDGE OF Γ-Aminocrotonic ACID. VINYLOGS OF Α-Amino ACIDS. I

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Determination of Free Amino Acids by Titration of the Carbon Dioxide Formed in the Reaction With Ninhydrin

Cited by 183 publications

References 5 publications

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B6 Antagonist from Flaxseed (Linum usitatissimum)*

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B6 Antagonist from Flaxseed (Linum usitatissimum)*

The Inorganic and Amino Acid Composition of Some Lamellibranch Muscles

CONTRIBUTION TO THE KNOWLEDGE OF Γ-Aminocrotonic ACID. VINYLOGS OF Α-Amino ACIDS. I

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Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*

Isolation, Characterization, and Synthesis of Linatine. A Vitamin B₆ Antagonist from Flaxseed (Linum usitatissimum)^*