Determination of Folate Patterns in Mouse Plasma, Erythrocytes, and Embryos by HPLC Coupled with a Microbiological Assay

A Parallel Processing Solid Phase Extraction Protocol for the Determination of Whole Blood Folate

“…This finding is consistent with the widely held belief that existing folate methods systematically underestimate red blood cell (RBC) folate due to inefficient release from RBC (3,(5)(6)(7)(8). Folate can be trapped by oxygenated hemoglobin and the RBC folate concentration will be underestimated.…”

“…Numerous studies have reported a large variance in estimates of folate content in whole blood using folate-binder radioassay compared to microbiological assay (3,6,8). The weak correlation between folatebinder radioassay to microbiological assay observed in this study is not uncommon.…”

“…A variety of methods for the measurement of blood folate are already described (1)(2)(3)(4)(5)(6). Variability and other concerns about the methods are also described (7)(8)(9).…”

Nanoparticle Targeting of Anticancer Drug Improves Therapeutic Response in Animal Model of Human Epithelial Cancer

“…The mice were maintained on a folate-deficient diet for the duration of the experiment to minimize the circulating levels of folic acid (25). The free folic acid level achieved in the serum of the mice before the experiment approximated human serum levels (26,27). Mice were evaluated at various time points (5 minutes to 7 days) following i.v.…”

Effects of dietary folic acid level and symbiotic folate production on fitness and development in the fruit flyDrosophilamelanogaster

“…34.19.9). 32 Preliminary experiments showed that 188 μl rat serum per larval sample produced sufficient conjugase activity to cleave all glutamate residues in the fly samples. Samples were incubated at 37°C for 2 hours to allow conjugase activity, then at 80°C for 5 minutes to denature conjugase, and then cooled on ice.…”