A Parallel Processing Solid Phase Extraction Protocol for the Determination of Whole Blood Folate

Determination of aldehydes in human breath by on-fibre derivatization, solid-phase microextraction and GC–MS

“…In another study, Phillips developed a qualitative method to examine the entire range of exhaled VOCs, including the aldehydes nonanal and hexanal [12]. Lin et al have described a method for the determination of aldehydes in breath, which involved derivatization on dinitrophenylhydrazine-impregnated silica cartridges, followed by HPLC/UV detection [13]. More recently, selected ion flow tube mass spectrometry has been used to quantify acetaldehyde in the breath of healthy volunteers [14].…”

Determination of Folate Vitamers in Human Serum by Stable-Isotope-Dilution Tandem Mass Spectrometry and Comparison with Radioassay and Microbiologic Assay

“…Although these chromatographic methods have better specificity than the microbiologic assay and immunoassay, they often lack sensitivity for the low concentrations of folate in serum and cannot be considered as high-order reference methods because they lack an appropriate internal standard (IS). The gas chromatography-mass spectrometry method used to analyze whole-blood folates showed greater sensitivity than previous chromatographic methods; however, gas chromatography-mass spectrometry requires complex sample preparation, including chemical derivatization, that introduces new sources of experimental error and increases the probability of folate degradation (25,26 ).…”

Measurement of Folates in Serum and Conventionally Prepared Whole Blood Lysates: Application of an Automated 96-Well Plate Isotope-Dilution Tandem Mass Spectrometry Method

“…Gas chromatography/mass spectrometry used to analyze WBF after cleavage of folates to p-aminobenzoic acid showed greater sensitivity than previous chromatographic methods and used for the first time an isotopelabeled internal standard (IS). The method, however, requires a complex and lengthy multistep sample preparation including chemical derivatization (11,(13)(14)(15). We describe here the first automated 96-well plate stableisotope-dilution liquid chromatography-tandem mass spectrometry (LC/MS/MS) method that measures intact folate monoglutamates in conventionally prepared whole blood (WB) lysates and serum.…”