Determination of fluoroquinolones in human urine by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry1

Ballesteros, O.; Toro, I.; Sanz‐Nebot, Victoria; Zafra-Gómez, A.; Vı́lchez, J.L.; Barbosa, J.

doi:10.1016/j.jchromb.2003.09.019

Cited by 64 publications

(38 citation statements)

References 26 publications

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“…A compound having the specified mass is fragmented, and ion fragments characteristic of the parent compound are monitored. As a result, analysis by mass spectrometry results in lower background noise, higher sensitivity and fewer drugmetabolite interferences compared with other analytical methods (Turpeinen and Stenman, 2003;Wallemacq et al, 2003;Ballesteros et al, 2003). Analysis by LC-MS-MS generally exhibits sensitivity in the range of 10 −6 -10 −12 g/mL plasma (Xia et al, 1999;Zhu et al, 2002;Phillips et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Analysis by LC-MS-MS generally exhibits sensitivity in the range of 10 −6 -10 −12 g/mL plasma (Xia et al, 1999;Zhu et al, 2002;Phillips et al, 2001). The utility of LC-MS-MS has been demonstrated in the analysis of many classes of drugs including antibiotics (Ballesteros et al, 2003), antidepressants (Naidong and Eerkes, 2004), and hypoglycemics (Lin et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Use of liquid chromatography-tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma

Kyle

Adcock

Krämer

et al. 2005

Biomed. Chromatogr.

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A method was developed for the quantitation of pentoxifylline [1-(5-oxohexyl)-3,7-dimethylxanthine] and a primary active metabolite, lisofylline [1-(5-hydroxyhexyl)-3,7-dimethylxanthine], using high-performance liquid chromatography (HPLC)-tandem mass spectrometry. This method was developed in order to overcome problems encountered with HPLC-ultraviolet detection. The operating parameters of the electrospray interface (PE SCIEX, TurboIon Spray) and lens voltages of the triple-quadrupole detector (PE SCIEX 365) were optimized in positive ion mode to obtain the best sensitivity of the analytes. Collision-induced dissociation was used to produce fragment ions, and multiple reaction monitoring was used to quantitate pentoxifylline (m/z 279/181) and lisofylline (m/z 263/181). Dichloromethane was used to extract the drug, metabolite, and the internal standard (3-isobutyl-1-methylxanthine) from plasma. A reverse-phase C8(2) 150 x 1.0 mm HPLC column was used to resolve all three compounds in less than 6 min. Calibration curves were generated using peak area and were linear from 1 to 1000 ng/mL (R(2) > 0.99). The small sample volume, ease of extraction, and sensitivity provide advantages over more conventional methods of quantitation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of liquid chromatography-tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma

Kyle

Adcock

Krämer

et al. 2005

Biomed. Chromatogr.

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“…[7][8][9] Metabolomics data for individuals were tabulated in a Microsoft Excel 2010 spreadsheet to create a matrix for principal component analysis (PCA) and statistical plots, including box plots and Quantile-quantile (Q-Q) plots. Using PCA, one of the projection-based methods, the multi-dimensional data table was converted into a low-dimensional model plane that approximates all observations.…”

Section: Discussionmentioning

confidence: 99%

Quantile Normalization Approach for Liquid Chromatography— Mass Spectrometry-based Metabolomic Data from Healthy Human Volunteers

et al. 2012

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In this study, 1-norm, 2-norm, and quantile normalization techniques were evaluated to compare the effects of three different normalization methods on liquid chromatography-mass spectrometry (LC-MS)-based metabolomics data. Experimental Study designThis randomized, single-blind, two-period, two-way crossover study with a 1-week washout period was conducted at the Clinical Trial Center of the Kyungpook National University Hospital, Daegu, Republic of Korea (Fig. 1). A total of eight subjects were randomly assigned to one of two treatment sequences: (1) 200 mg of cyclosporine (high dose) in the first study period, followed by 2 mg of cyclosporine (low dose) in the second study period, or (2) 2 mg cyclosporine (low dose) in the first study period, followed by 200 mg cyclosporine (high dose) in the second study period. In each period, all subjects were hospitalized 24 h before and 24 h after drug administration (Fig. 1). Pre-dose and post-dose urine samples for metabolomic profiling were collected over the 24-h period before and after oral administration of cyclosporine.

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“…Different analytical methods such as capillary electro kinetic chromatography [13][14][15][16], solid phase spectrofluorimetry [17], and high performance liquid chromatography with fluorescent or UV detection [18][19][20][21][22][23][24][25][26][27] have been developed to measure the levofloxacin concentrations in plasma, urine, bile, faeces and other biological fluids [28]. To our knowledge, so far only five studies [29][30][31][32][33] have quantified levofloxacin concentrations by LC-MS/MS; however, among these studies some studies measured levofloxacin in human urine [27], other studies used plasma samples but did not provide description on run time and the ones that did had a relatively long run time and sample preparation time. Therefore, to maximize the efficiency of existing LC-MS/MS methods and further compliment TDM, we aimed to develop and validate a rapid, selective, simple and robust method without extensive sample processing and short run times, to quantify levofloxacin and its metabolite desmethyl-levofloxacin in the serum of MDR-TB patients.…”

Section: Introductionmentioning

confidence: 99%

Determination of levofloxacin in human serum using liquid chromatography tandem mass spectrometry

Ghimire¹,

Hateren²,

Vrubleuskaya³

et al. 2018

J Appl Bioanal

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A rapid liquid chromatography tandem-mass spectrometry method was developed for the determination of levofloxacin and its metabolite (desmethyl-levofloxacin) in human serum. Sample preparation was done using protein precipitation technique. Our method had a run time of 2.5 min and retention times of 1.6 min for all analytes. The standard curves were linear within the concentration range of 0.10 to 5.00 mg/L for levofloxacin and 0.10 to 4.99 mg/L for desmethyllevofloxacin; a correlation coefficient (R 2 ) of 0.999 and 0.998 respectively. The lower limit of quantification for both analytes was 0.10 mg/L. Within-day precision ranged from 1.4% and 2.4% for levofloxacin, 1.5% to 5% for desmethyl-levofloxacin and between-day precision ranged from 3.6% to 4.1% for levofloxacin and 0.0% to 3.3% for desmethyl-levofloxacin; whereas, accuracy ranged from 0.1% to 12.7% for levofloxacin and 0.2% to 15.6% for desmethyl-levofloxacin. This method could be a useful asset for routine therapeutic drug monitoring of levofloxacin in multi-drug resistant tuberculosis patients.

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Determination of fluoroquinolones in human urine by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry1

Cited by 64 publications

References 26 publications

Use of liquid chromatography-tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma

Use of liquid chromatography-tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma

Quantile Normalization Approach for Liquid Chromatography— Mass Spectrometry-based Metabolomic Data from Healthy Human Volunteers

Determination of levofloxacin in human serum using liquid chromatography tandem mass spectrometry

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