Determination of fluoroquinolones in blood by matrix solid‐phase dispersion extraction and CE

Abstract

A simple and rapid method for the determination of residues of four fluoroquinolones in blood samples was developed. The method was based on matrix solid-phase dispersion extraction followed by CE with ultraviolet detection. 1-Butyl-3-methylimidazolium tetrafluoroborate aqueous solution was used as the background electrolyte for the separation of fluoroquinolones. The average recoveries of the four fluoroquinolones at two spiked levels ranged from 54.0 to 86.4% for pig blood, and 45.4 to 79.9% for deer blood, with the relative standard deviations <9.45%. Detection limits for the four fluoroquinolones in blood sample ranged from 0.15 to 0.31 μg/mL.

Recent advances in the analysis of antibiotics by CE and CEC

“…An MSPD extraction was reported as cleanup and preconcentration step for the determination of four fluoroquinolones by CZE‐UV 19. This technology involves mechanically blending of a small amount of sample matrix with an appropriate sorbent followed by washing and elution of compounds with a small amount of solvent.…”

“…The most extended ones are liquid–liquid extraction (LLE), SPE, and solid–liquid extraction. Aside from these common techniques, there are also some studies in which inline extraction methods have been employed 7: magnetic SPE (MSPE) 4, dispersive liquid–liquid microextraction (DLLME) 16, molecular imprinted SPE (MISPE) 17, single drop liquid–liquid–liquid microextraction (SD‐LLLME) 18, matrix solid‐phase dispersion (MSPD) 19, and poly‐[(methacrylic acid‐ co ‐ethylene glycol dimethacrylate)] monolith microextraction (PMME) 11.…”

Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection

“…It permits complete dispersion of sample with the aid of shearing forces generated by blending process and offers exhaustive extraction, clean-up and concentration of analytes in a single step, which eliminates the most of the complications of performing classical SPE process and making sample preparation easier and faster. It drastically shortens the analysis time and reduces the consumption of toxic and expensive solvents, especially for solid, semisolid and highly viscous biological samples [22,24,25]. However, the routine dispersants for extracting MEL, such as mixed-mode cation exchange (MCX), polymer cation exchange (PCX), and strong cation exchange (SCX), are lack selectivity for analytes, which would lead to the coextraction of interferents and affect the quantification of analytes.…”

Highly-sensitive detection of eight typical fluoroquinolone antibiotics by capillary electrophoresis-mass spectroscopy coupled with immunoaffinity extraction

“…The reported cleanup methods for FQs include solid phase extraction (SPE), 6,11,12,15 pressurized liquid extraction (PLE), 19,20 matrix solid-phase dispersion (MSPD), 21,22 microwave-assisted extraction, 23,24 microdialysis. 25 The most common used SPE was reverse-phase SPE based upon the hydrophobic interaction between the analytes and the sorbents, which was lack of selectivity, leading to the co-extraction of analytes and matrix interference, especially when interested analytes are at trace levels and interference at higher concentration.…”