Determination of fluoroquinolone antibiotics by microchip capillary electrophoresis along with time-resolved sensitized luminescence of their terbium(III) complexes

Abstract

We report on the time-resolved detection of the three fluoroquinolone (FQs) antibiotics ciprofloxacin (CIP), enrofloxacin (ENR) and flumequine (FLU). On addition of terbium(III) ions, the terbium(III)-FQs chelates are formed insitu in an on-capillary derivatization reaction of a microfluidic system. The laser-induced terbium(III)-sensitized luminescence of the chelates is measured at excitation/emission wavelengths of 337/545 nm. The analytes can be separated and quantified within less than 4 min. A solid phase extraction step for analyte preconcentration can be included prior to chelation and microchip capillary electrophoresis. The analytical ranges of the calibration graphs for CIP, ENR and FLU are from 10.6 to 60.0, 10.3 to 51.0, and 11.5 to 58.8 ng mL −1 , respectively, and the detection limits are 3.2, 3.1 and 3.6 ng mL −1 , respectively. The precision was established at two concentration levels of each analyte and revealed relative standard deviations in the range from 3.0 to 10.2 %. The method was applied to the analysis of FQ-spiked water samples.

Analysis of antibiotics by CE and their use as chiral selectors: An update

“…Table 4 summarizes the applications to the determination of antibiotics in the period reviewed in this article. As it can be observed, ED [9] and LIF [10,11] have been the selected detection systems for chip-based CE. Application of these devices to river [10] and tap waters [11] or biological samples [9] has been performed showing the usefulness of these devices to determine antibiotics in real samples.…”

Derivatisation for separation and detection in capillary electrophoresis (2012–2015)

“…Twelve articles were considered from this mode . Mesbah et al.’s strategy for in‐capillary labelling of nanomolar‐level ubiquitin in cerebrospinal fluid with Fluoprobes® 488 N ‐hydroxysuccinimide is shown in Fig.…”

“…Derivatisation in CE is used to improve or enable detection by LIF, UV, chemiluminescence (CL), electrochemiluminescence (ECL), amperometric detection (AD), MS or [16-18, [20,33,35,36,38,42-48], [50,51,57]], two on CL [22,49], one on ECL [39], two on AD [26,58], three on MS (and MS combined with other detectors) [25,32,37] and two on C4D [40,41].…”

“…Hexamethylenetetramine enhanced the ECL signal of Tris(2,2 -bipyridyl)ruthenium(II) complex and allowed a detection limit of 0.15 g/m 3 . Sierra-Rodero et al analysed three fluoroquinolone antibiotics from water samples by terbium(III)-induced luminescence set up on a microchip [49]. This system allowed rapid separation in less than 4 min with detection limits in the low nanograms per millilitre range.…”

Polymethacrylate microparticles covalently functionalized with an ionic liquid for solid-phase extraction of fluoroquinolone antibiotics

“…The identification and quantification of FQs in various biological matrices are important to ensure that they are not present at levels that may pose health risks to people [13,14]. Several methods have been developed for analysis of these drugs in various biological matrices mainly including liquid chromatography [11,12,15], capillary electrophoresis [16][17][18], liquid chromatography-mass spectrometry [19][20][21][22] and microbiological assay [23]. However, due to the complexity of biological matrices, it is difficult to achieve direct separation of target analytes from complex biological matrices.…”