Determination of (fluoro)quinolone antibiotic residues in pig kidney using liquid chromatography–tandem mass spectrometry

Toussaint, B.; Chedin, Mostafa; Bordin, Guy; Rodríguez, A. R.

doi:10.1016/j.chroma.2005.02.057

Cited by 85 publications

(28 citation statements)

References 24 publications

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“…However, these methods are complicated, timeconsuming, and specificity-lacking (Kurittu et al, 2000). Instrumental methods, including liquid chromatography-mass spectrometry (LC-MS) and highperformance liquid chromatography (HPLC) (Johnston et al, 2002;van Vyncht et al, 2002;EspinosaMansilla et al, 2005;Toussaint et al, 2005a;2005b;Zeng et al, 2005), are the most current widely used methods for CPFX residues detection. These methods are sensitive and highly specific, but they require expensive equipment, large volumes of solvents, and highly trained individuals for operating complicated instruments, with time-consuming sample cleanup processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Huang

Yin

Lü

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC 50 ) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%, with coefficients of variation of less than 12.2%.

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Section: Introductionmentioning

confidence: 99%

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Huang

Yin

Lü

et al. 2010

J. Zhejiang Univ. Sci. B

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“…The mobile phase consisted of a mixture of 0.1% formic acid in acetonitrile and 0.1% formic acid in water. Several gradient programs were tested to ensure optimum quinolones separation on each of the studied chromatographic columns taking into account that two of the studied quinolones have the same molecular mass and may interfere in the case of co-elution [37,38]. The results showed that all of the studied quinolones can be separated using the same gradient program on the two studied core-shell particles based columns: Kinetex PFP and Poroshell 120 EC-C18 (Table I).…”

Section: High-performance Liquid Chromatography-tandem Mass Spectromementioning

confidence: 99%

“…Although the separation time on Gemini column was higher than other studied columns, it is short enough to ensure good separation at low reagent and analysis time consumption. The MS/MS detection parameters were also optimized starting with the parameters described in [37,39]. In our work, two product ions for each determined compound have been selected (from their corresponding precursor ions), which complies with the requirements proposed in current EU legislation for a minimum total score of 3 identification points for confirmatory MS method [35].…”

Section: High-performance Liquid Chromatography-tandem Mass Spectromementioning

confidence: 99%

Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

Stoilova¹,

Surleva²,

Stoev³

2014

Acta Chromatographica

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Summary. High-performance liquid chromatography coupled with fluorescence (HPLC-FD) and tandem mass spectrometric detection (LC-MS/MS) was studied as a versatile tool for fast and reliable determination of nine regulated quinolones in food of animal origin (Council Regulation 2377/90/ECC). The sample pre-treatment protocol includes double step extraction with acetonitrile followed by solid phase extraction (SPE) cleanup on hydrophobic-lipophilic balance (HLB) cartridge. The separation of quinolones in HPLC-FD determination was performed on C18 Zorbax column with a gradient mixture of aqueous formic acid, methanol, and acetonitrile. A multi-wavelength excitation/emission program was used for sensitive quinolones detection. The separation efficiency of newly available chromatographic columns: Gemini C18 and Synergi Polar RP (fully porous particles), as well as Kinetex PFP and Poroshell 120 EC-C18 (coreshell particles), was studied in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis. Appropriate gradient elution program was designed for each column. Multiple reaction monitoring was used for selective determination of each quinolone. LC-MS/MS allowed quinolones determination in less than 5 min. Both methods showed detection limits below maximum residue limits for quinolones residues in food commodities.

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“…Toussaint et al developed a method for isolating 11 quinolones from pig kidney [306]. Other groups have developed methods for isolating quinolone residues from animal tissues [307]. More novel methods have also been developed including hot water extraction [87].…”

Section: Quinolonesmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

181

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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Determination of (fluoro)quinolone antibiotic residues in pig kidney using liquid chromatography–tandem mass spectrometry

Cited by 85 publications

References 24 publications

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

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