Determination of fifteen coccidiostats in feed at carry-over levels using liquid chromatography–mass spectrometry

Pietruk, Konrad; Olejnik, Małgorzata; Jedziniak, Piotr; Szprengier-Juszkiewicz, Teresa

doi:10.1016/j.jpba.2015.03.019

Cited by 39 publications

(22 citation statements)

References 20 publications

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“…또한 배출된 의약품 일부는 생물학적 활성도가 그대로 유지되기도 한다 (MartinezCarballo et al, 2007). Kroker (1983)는 돼지가 베타-락탐계 Martinez-Carballo et al, 2007;McArdell et al, 2003;Tolls, 2001 and Samanidou, 2013;Lopes et al, 2012;Xu et al, 2012), 육류 (Berendsen et al, 2013;Bousova et al 2013;Tao et al 2012), 가축사료 (Amelin and Timofeev, 2016;Boscher et al, 2010;Pietruk et al 2015) 등의 고체 시료를 대상으로 전처 리방법을 개발·적용한 사례가 있으나 가축분뇨 중의 항생제 분석방법 (Gorissen et al 2015;Zhou et al, 2012) (Chitescu et al, 2015;Gros et al, 2013;Iglesias et al, 2012;Shelver et al, 2010). 고상추출법은 일반적으로 Fig 편적으로 적용 가능한 최적 전처리조건을 도출하기 위한 다 양한 연구가 진행되어 왔다 (Ben et al, 2008;De Alwis and Heller, 2010;Gros et al, 2013;Iglesias et al, 2012;Zhou et al, 2012 (Ben et al, 2008;Gros et al, 2013;Shelver et al, 2010;Zhou et al, 2012) …”

Section: 축산업에서는 항생제를 주로 사용하며 사료나 물에 섞어 섭취하게 하는데 대부분의 항생제는 흡수나 대사 작용을 unclassified

Determination of Antibiotic Residues: II. Extraction and Clean-up Methods for Liquid Samples_A Review

Kim¹,

Ryu²,

Chung³

et al. 2016

Journal of Korean Society on Water Environment

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Increased attention has been paid to the presence of veterinary antibiotics in various environmental matrices due to their toxicological behavior in the ecosystem and development of antibiotic-resistant strains of pathogenic bacteria. In the this review, 37 target antimicrobials were selected based on annual sales of antibiotics for livestock in South Korea 2014. Also, extraction and clean-up methods for the determination of the antibiotic residues in liquid samples including water, milk, and honey were comprehensively reviewed in the literature. Solid-phase extraction (SPE) was commonly used as a pre-treatment method for the samples. Most of the analytes were extracted in acidic conditions (2.5~4.0) except for aminoglycosides, which were extracted in neutral conditions (7.0~8.0). β-Lactams showed the highest recoveries in neutral pH due to their degradation characteristics in acidic media. Starta-X, Oasis HLB, and Oasis MCX were frequently applied as an SPE cartridge and Oasis HLB showed the highest recoveries for the majority of antibiotic classes. The homogenized honey and milk were extracted by mixing with acids for deproteinization. Solids and other interfering substances in the extract were eliminated by centrifugation followed by membrane filtration or SPE before injection into HPLC.

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Section: 축산업에서는 항생제를 주로 사용하며 사료나 물에 섞어 섭취하게 하는데 대부분의 항생제는 흡수나 대사 작용을 unclassified

Determination of Antibiotic Residues: II. Extraction and Clean-up Methods for Liquid Samples_A Review

Kim¹,

Ryu²,

Chung³

et al. 2016

Journal of Korean Society on Water Environment

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“…The European Union [5] has allowed anticoccidial drugs as additives for the production of poultry feed, but use in the production of laying hens is banned. However, anticoccidial residues are often found in eggs, which may be the result of cross-contamination from feed production [6][7][8]. Studies have shown that anticoccidial drugs in feed will be transferred to eggs [9].…”

Section: Introductionmentioning

confidence: 99%

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

et al. 2020

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A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrometry was used to quantitatively and qualitatively analyze the target compounds via electrospray ionization (ESI+) and multiple reaction monitoring mode. The HPLC–MS/MS and UPLC–MS/MS methods were validated according to the requirements defined by the European Union and the Food and Drug Administration. The limits of detection and limits of quantification of the eight coccidiostats in eggs were 0.23–0.52 µg/kg and 0.82–1.73 µg/kg for HPLC–MS/MS, and 0.16-0.42 µg/kg and 0.81-1.25 µg/kg for UPLC–MS/MS, respectively. The eggs were spiked with four concentrations of the eight coccidiostats, and the HPLC–MS/MS and UPLC–MS/MS average recoveries were all higher than 71.69% and 72.26%, respectively. Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision. Finally, the HPLC–MS/MS and UPLC–MS/MS methods were successfully applied to detect eight coccidiostats in 40 eggs.

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“…Illegal use and failure to comply with specified withdrawal periods before human consumption may result in drug residues in edible animal tissues, posing a potential health risk to consumers. Numerous methods, including high performance liquid chromatography (Amelin & Timofeev, 2016;Barreto, Ribeiro, Hoff, & Dalla Costa, 2017;Clarke et al, 2013), gas chromatography (Ekstrom & Kuivinen, 1984;Newkirk & Barnes, 1989), and liquid chromatography tandem mass spectrometry (Matus & Boison, 2016;Pietruk, Olejnik, Jedziniak, & Szprengier-Juszkiewicz, 2015), have been used for the determination of coccidiostats in milk, eggs, and animal tissue have been reported. Although sensitive and specific, because these methods are rather time-consuming and require complicated sample preparation and expensive instruments, they are unsuitable for screening large numbers of samples (Khaemba et al, 2016;Kong et al, 2017;Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017;Peng, Liu, Kuang, Cui, & Xu, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk

Song

Suryoprabowo

Liu

et al. 2019

Food and Agricultural Immunology

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A highly sensitive, specific, and rapid immunochromatographic assay for halofuginone (HFG) has been developed. A well-characterized immunogenic conjugate of HFG with bovine serum albumin was produced using the N,N ′′-Carbonyldiimidazole method. A sensitive monoclonal antibody specific for HFG was then generated by immunizing BALB/c mic with the HFG conjugate. The antigen, HFG-CDI-ovalbumin and goat anti-mouse IgG were attached to a nitrocellulose membrane as the test line and the control line, respectively to produce an immunochromatographic test strip for the rapid detection of HFG in milk. Under optimized conditions, the detection limits of the semi-quantitative test strip for HFG were as low as 50 ng/mL in 0.01 M PBS (pH 7.4) and 100 ng/mL in milk. All the results were obtained within 5 min. The newly developed immunochromatographic assay provides a sensitive, rapid, and specific procedure for on-site screening for HFG.

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Determination of fifteen coccidiostats in feed at carry-over levels using liquid chromatography–mass spectrometry

Cited by 39 publications

References 20 publications

Determination of Antibiotic Residues: II. Extraction and Clean-up Methods for Liquid Samples_A Review

Determination of Antibiotic Residues: II. Extraction and Clean-up Methods for Liquid Samples_A Review

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk

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