Determination of extended spectrum beta-lactamases, metallo-beta-lactamases and AmpC-beta-lactamases among carbapenem resistant Pseudomonas aeruginosa isolated from burn patients

Kalantar-Neyestanaki, Davood; Mirsalehian, Akbar; Rezagholizadeh, Fereshteh; Jabalameli, Fereshteh; Taherikalani, Morovat; Emaneini, Mohammad

doi:10.1016/j.burns.2014.02.010

Cited by 62 publications

(34 citation statements)

References 30 publications

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“…AmpC β-lactamase, which is a type of cephalosporinase (1), is known to be responsible for antimicrobial resistance in gram-negative bacilli (2). The knowledge of potential risk factors for that resistance may help to limit its impact by enabling the implementation of effective control measures and judicious antimicrobial therapy (3).…”

Section: Introductionmentioning

confidence: 99%

Detection and genotype analysis of AmpC β-lactamase in Klebsiella pneumoniae from tertiary hospitals

Liu

2016

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the phenotype and genotype of plasmid-mediated AmpC (pAmpC) β-lactamase in Klebsiella pneumoniae and its antibiotic resistance. A total of 130 non-repetitive clinical isolates of Klebsiella pneumoniae, obtained from tertiary hospitals, were phenotypically screened for pAmpC β-lactamase production with the cefoxitin disk diffusion test. β-lactamase genes in the screened isolates were detected using multiplex polymerase chain reaction (PCR); carbapenemase genes in pAmpC β-lactamase-producing isolates that were resistant to imipenem were detected using PCR. Out of the 130 isolates of Klebsiella pneumoniae, 62 strains (47.7%) were resistant to cefoxitin, including 14 strains (10.8%) positive for pAmpC β-lactamase (DHA type), among which 12 strains (85.7%) were susceptible to imipenem, and 2 strains, which were carrying Klebsiella pneumoniae carbapenemase (KPC)-2 gene, were resistant to imipenem. The pAmpC β-lactamase-producing Klebsiella pneumoniae isolates from the tertiary hospitals were mainly of DHA-1 genotype, and the majority were susceptible to carbapenems; drug-resistant strains were associated with KPC-2 expression. IntroductionAmpC β-lactamase, which is a type of cephalosporinase (1), is known to be responsible for antimicrobial resistance in gram-negative bacilli (2). The knowledge of potential risk factors for that resistance may help to limit its impact by enabling the implementation of effective control measures and judicious antimicrobial therapy (3). AmpC β-lactamases are either plasmid-or chromosomal-mediated (4). With regard to plasmid-mediated AmpC (pAmpC), no single phenotypic method is satisfactory for its detection; the combined application of phenotypic tests is necessary for the screening and confirmation of the presence of pAmpC-mediated resistance (5). Chromosomal-mediated AmpC hyperproducing Escherichia coli (E. coli) continues to be mostly susceptible to third-generation cephalosporins (6). Polymerase chain reaction (PCR) remains the gold standard for the detection of AmpC β-lactamases (7). As β-lactam antibiotics are widely used, the production of AmpC β-lactamases by bacteria results in considerable antibiotic resistance (8).Resistance to broad-spectrum β-lactams mediated by extended spectrum broad-spectrum β-lactamases (ESBLs) and AmpC β-lactamase enzymes is an increasing problem worldwide (9). In Portugal, 104/124 (83.9%) of Enterobacteriaceae isolates that were resistant to third generation cephalosporins were also resistant to fourth generation cephalosporins (10). In comparison with ESBL-producing gram-negative bacilli, AmpC β-lactamase-producing strains show more extensive antibiotic resistance; 29% of Acinetobacter strains, for example, were found to produce both ESBL and AmpC enzymes (11). To be more specific, the majority of Acinetobacter baumannii isolates were producers of carbapenemase and metallo-β-lactamase (12). In a study of isolates collected between 2002 and 2008, DHA-1 was the most prevalent acquired AmpC (94%)...

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Section: Introductionmentioning

confidence: 99%

Detection and genotype analysis of AmpC β-lactamase in Klebsiella pneumoniae from tertiary hospitals

Liu

2016

Experimental and Therapeutic Medicine

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“…The genes encoding ESBLs (bla TEM , bla SHV , bla CTX-M , and bla PER ), AmpC (bla ACC , bla FOX , bla MOX , bla DHA , bla CIT , and bla EBC ), and carbapenemase (bla IMP , bla VIM , bla NDM , bla KPC , bla GIM , and bla OXA-48 ) were targeted by polymerase chain reaction (PCR) using specific primers 10,11 . The detection of seven different virulence genes (csgA, csgB, csgD, rpos, FliI, fepA, and iutA) was performed with PCR using the oligonucleotide primers listed in Table 1.…”

Section: Detection Of β-Lactamases and Virulence Genesmentioning

confidence: 99%

“…The minimum inhibitory concentration (MIC) of imipenem was determined by the microbroth dilution method according to CLSI criteria 9 . AmpC overproduction was confirmed according to the method of Kalantar-Neystanaki et al 10 .…”

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confidence: 99%

Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

Ghanavati

Emaneini

Kalantar-Neyestanaki

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Introduction: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. Methods: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. Results: The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. Conclusions: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.

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“…Hence, skin damage along with compromised blood supply around wound and immunodeficiency increase acquisition of nosocomial infection in burn patients [1,7-resistant strains thus limiting therapeutic options [3,13,[16][17][18][19]. The primary cause for production of multiple drug resistant strains is β-lactamases particularly extended spectrum β-lactamases (ESBL) whose incidence is steadily increasing all over the world [9,16,[19][20][21][22][23][24]. Most prevalent types of ESBL are TEM (TEM1-2), SHV-1 and CTX-M which are produced by Gram negative bacilli.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility patterns and CTX-M β-lactamase producing clinical isolates from burn patients in Islamabad, Pakistan

Hussain¹,

Munir²,

Fatima³

et al. 2017

APJTD

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Determination of extended spectrum beta-lactamases, metallo-beta-lactamases and AmpC-beta-lactamases among carbapenem resistant Pseudomonas aeruginosa isolated from burn patients

Cited by 62 publications

References 30 publications

Detection and genotype analysis of AmpC β-lactamase in Klebsiella pneumoniae from tertiary hospitals

Detection and genotype analysis of AmpC β-lactamase in Klebsiella pneumoniae from tertiary hospitals

Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

Antimicrobial susceptibility patterns and CTX-M β-lactamase producing clinical isolates from burn patients in Islamabad, Pakistan

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