Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

Ghanavati, Roya; Emaneini, Mohammad; Kalantar-Neyestanaki, Davood; Sattari-Maraji, Azin; Dalvand, Mosayyeb; Beigverdi, Reza; Jabalameli, Fereshteh

doi:10.1590/0037-8682-0227-2017

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…This finding differs with the studies of Nepalwhich reported meropenem resistance 8.8% and imipenem resistance was 10.5%. 9,13 Higher percentage of resistance in this study may be due to higher rate of use of meropenem in our hospitals. In this study, majority of urinary Enterobacter spp.…”

Section: Discussionmentioning

confidence: 63%

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Antimicrobial Resistance Pattern of Enterobacter Species Isolated from Different Clinical Specimens in a Tertiary Care Hospital of Bangladesh

Quraishi

Saleh

Roy

et al. 2019

Bangladesh J Med Microbiol

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The present study was undertaken to determine the antimicrobial resistance pattern of Enterobacter species to guide the clinician in selecting the best antimicrobial agent for an individual patient. A total of 50 clinical isolates of Enterobacter species were collected from different clinical specimens at the microbiology laboratory of BSMMU between August, 2018 and September, 2019. The two main species of Enterobacter, E.cloacae and E.aerogenes were identified by biochemical tests. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method and reported according to CLSI guidelines. Majority (56%) of the isolated Enterobacter were E.cloacae, 40% were E.aerogenes and 4% were other species. The Enterobacter isolates showed relatively high resistance rates to the cephalosporins including cefoxitin (82%), cefixime (62%), ceftazidime (46%) and ceftriaxone (46%). Resistance to the carbapenems and aminoglycosides was relatively low. The high resistance rates of Enterobacter species to multiple antibiotics makes it necessary for antimicrobial susceptibility testing to be conducted prior to antibiotic prescription. Bangladesh J Med Microbiol 2019; 13 (2): 3-6

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Section: Discussionmentioning

confidence: 63%

“…A study in Iran reported 77.1% isolated Enterobacter as E.cloacae and 22.8% isolates as E.aerogenes. 9 Another study in China reported 68.2% blood isolates were E.cloacae and 26.4% blood isolates were E.aerogenes. 10 Most of the Enterobacter isolates (68%) were obtained from urine in this study.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Pattern of Enterobacter Species Isolated from Different Clinical Specimens in a Tertiary Care Hospital of Bangladesh

Quraishi

Saleh

Roy

et al. 2019

Bangladesh J Med Microbiol

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“…Our K. aerogenes CRKA317 harbored two copies of bla AmpC gene. Several Gram-negative organisms, including E. aerogenes , Enterobacter cloacae , Serratia marcescens , Providencia stuartii , Pseudomonas aeruginosa , Hafnia alvei , and Morganella morganii , have presented AmpC in their genomes ( Jacoby, 2009 ; Ghanavati et al, 2018 ; Tamma et al, 2019 ). This enzyme provides resistance against aminopenicillins, cephalosporins, oxyimino-cephalosporins (e.g., ceftriaxone, cefotaxime, and ceftazidime), cephamycins (e.g., cefoxitin and cefotetan), and monobactams (aztreonam) ( Jacoby, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

First report of coexistence of blaKPC-2 and blaNDM-1 in carbapenem-resistant clinical isolates of Klebsiella aerogenes in Brazil

Rodrigues,

Nunes,

Soares

et al. 2024

Front. Microbiol.

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Klebsiella aerogenes is an important opportunistic pathogen with the potential to develop resistance against last-line antibiotics, such as carbapenems, limiting the treatment options. Here, we investigated the antibiotic resistance profiles of 10 K. aerogenes strains isolated from patient samples in the intensive-care unit of a Brazilian tertiary hospital using conventional PCR and a comprehensive genomic characterization of a specific K. aerogenes strain (CRK317) carrying both the blaKPC-2 and blaNDM-1 genes simultaneously. All isolates were completely resistant to β-lactam antibiotics, including ertapenem, imipenem, and meropenem with differencing levels of resistance to aminoglycosides, quinolones, and tigecycline also observed. Half of the strains studied were classified as multidrug-resistant. The carbapenemase-producing isolates carried many genes of interest including: β-lactams (blaNDM-1, blaKPC-2, blaTEM-1, blaCTX-M-1 group, blaOXA-1 group and blaSHVvariants in 20-80% of the strains), aminoglycoside resistance genes [aac(6’)-Ib and aph(3’)-VI, 70 and 80%], a fluoroquinolone resistance gene (qnrS, 80%), a sulfonamide resistance gene (sul-2, 80%) and a multidrug efflux system transporter (mdtK, 70%) while all strains carried the efflux pumps Acr (subunit A) and tolC. Moreover, we performed a comprehensive genomic characterization of a specific K. aerogenes strain (CRK317) carrying both the blaKPC-2 and blaNDM-1 genes simultaneously. The draft genome assembly of the CRK317 had a total length of 5,462,831 bp and a GC content of 54.8%. The chromosome was found to contain many essential genes. In silico analysis identified many genes associated with resistance phenotypes, including β-lactamases (blaOXA-9, blaTEM-1, blaNDM-1, blaCTX-M-15, blaAmpC-1, blaAmpC-2), the bleomycin resistance gene (bleMBL), an erythromycin resistance methylase (ermC), aminoglycoside-modifying enzymes [aac(6’)-Ib, aadA/ant(3”)-Ia, aph(3’)-VI], a sulfonamide resistance enzyme (sul-2), a chloramphenicol acetyltransferase (catA-like), a plasmid-mediated quinolone resistance protein (qnrS1), a glutathione transferase (fosA), PEtN transferases (eptA, eptB) and a glycosyltransferase (arnT). We also detected 22 genomic islands, eight families of insertion sequences, two putative integrative and conjugative elements with a type IV secretion system, and eight prophage regions. This suggests the significant involvement of these genetic structures in the dissemination of antibiotic resistance. The results of our study show that the emergence of carbapenemase-producing K. aerogenes, co-harboring blaKPC-2 and blaNDM-1, is a worrying phenomenon which highlights the importance of developing strategies to detect, prevent, and control the spread of these microorganisms.

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“…The extended-spectrum cephalosporin resistant (ESC R ) Enterobacteriaceae pose a serious infection control challenge for public health [45]. The appearance of the ESC R phenotype is mainly promoted through plasmid-mediated lateral extended-spectrum β-lactamases (ESBLs) and AmpC gene transfer within Enterobacteriaceae [46].…”

Section: Discussionmentioning

confidence: 99%

Influence of Debaryomyces hansenii on bacterial lactase gene diversity in intestinal mucosa of mice with antibiotic-associated diarrhea

Tang

Peng

et al. 2019

PLoS ONE

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AimThe current study aimed to investigate the effects of Debaryomyces hansenii on the diversity of bacterial lactase gene in the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice.MethodsEighteen mice were randomly divided into three groups (6 mice per group): healthy control group, diarrhea model group and D. hansenii treatment group. The antibiotic-associated diarrhea model was established by intragastric administration with a mixture of cephradine and gentamicin sulfate (23.33 mL·kg-1·d-1) twice a day for 5 days continuously. After establishing the AAD model, the mice in the D. hansenii treatment group were gavaged with D. hansenii for three days, while other groups were gavaged with distilled water. Then, the intestinal mucosa of all three groups was collected and DNA was extracted in an aseptic environment for the following analysis.ResultsThe difference in the richness and homogeneity of the bacterial lactase gene among all samples were inapparent, as the difference in the Chao1, ACE, Simpson and Shannon indices among the three groups were insignificant (P>0.05). NMDS analysis also showed that the distance of the samples among the three groups was unobvious. Furthermore, the bacterial lactase gene in the mucosa mainly originated from Actinobacteria, Firmicutes and Proteobacteria. Compared with the healthy control group, the abundance of lactase genes originating from Cupriavidus, Lysobacter, Citrobacter, Enterobacter and Pseudomonas was increased in the D. hansenii treatment group, while the lactase gene from Acidovorax and Stenotrophomonas decreased (p < 0.01 or p < 0.05) in the diarrhea model group and the D. hansenii treatment group.ConclusionD. hansenii was capable of improving the growth of some key lactase-producing bacteria like Deinococcus, Cupriavidus and Lysobacter for treating AAD.

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Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

Cited by 9 publications

References 15 publications

Antimicrobial Resistance Pattern of Enterobacter Species Isolated from Different Clinical Specimens in a Tertiary Care Hospital of Bangladesh

Antimicrobial Resistance Pattern of Enterobacter Species Isolated from Different Clinical Specimens in a Tertiary Care Hospital of Bangladesh

First report of coexistence of blaKPC-2 and blaNDM-1 in carbapenem-resistant clinical isolates of Klebsiella aerogenes in Brazil

Influence of Debaryomyces hansenii on bacterial lactase gene diversity in intestinal mucosa of mice with antibiotic-associated diarrhea

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