Determination of Ephedrine Alkaloids in Dietary Supplement Standard Reference Materials

Sander, Lane C.; Sharpless, Katherine E.; Satterfield, Mary B.; Ihara, Toshihide; Phinney, Karen W.; Yen, James H.; Wise, Stephen A.; Gay, Martha L.; Lam, Joseph W. H.; McCooeye, Margaret; Gardner, Graeme J.; Fraser, Catharine; Sturgeon, Ralph E.; Roman, Mark

doi:10.1021/ac0484530

Cited by 51 publications

(46 citation statements)

References 15 publications

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“…1). An overview of different methodologies (LC-UV, LC-MS, LC-MS-MS and CE) for the analysis of Ephedra alkaloids was presented by Sander et al [19], whereas Phinney et al [20] compared the effects of different cyclodextrins on this separation problem. They concluded that the combination of S-X-CD and DM-X-CD will be most suitable as additive.…”

Section: Alkaloidsmentioning

confidence: 99%

Quality control of herbal medicines by capillary electrophoresis: Potential, requirements and applications

Ganzera

2008

Electrophoresis

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Herbal preparations, particularly those from traditional Chinese or Indian medicine, are becoming increasingly popular in Europe and the USA. Their application is often based on long-term historic use rather than on scientific evidences; thus, analytical tools to assure their efficacy, safety and consistency are in great demand. This review evaluates the importance of CE for quality control of herbal medicinal products during the last five years. After briefly describing the general characteristics of natural products analysis by CE, numerous applications on medicinal plants or herbal products are summarized. These examples not only reflect the enormous variability of CE with respect to buffer systems and detection modes employed, but also indicate an increasing importance of this separation technique for quality control purposes compared with more established ones such as HPLC.

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Section: Alkaloidsmentioning

confidence: 99%

Quality control of herbal medicines by capillary electrophoresis: Potential, requirements and applications

Ganzera

2008

Electrophoresis

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“…Recently, the National Institute of Standards and Technology (NIST) issued a suite of five ephedra-containing dietary supplements standard reference materials (SRMs) with certified values for ephedrine alkaloids, synephrine, and caffeine [7]. The materials constitute various types of natural, extracted, and processed sample matrices.…”

Section: (1r2s)-(-)-ephedrine [(-)-Ep] (1s2s)-(1)-pseudoephedrine mentioning

confidence: 99%

“…Hence, accurate and sensitive analysis of such samples is of significant challenge to analytical chemists. Sandar et al published certified values of six ephedrine alkaloids of interest [(-)-EP, (1)-c-EP, (-)-Nor-EP, (1)-Nor-c-EP, (-)-Me-EP, and (1)-Me-c-EP] which were independently measured by different laboratories using several methods such as LC-UV, LC-MS, LC-MS/MS and CE [7]. In addition, Phinney and co-workers developed three chiral CE-UV methods using either single or dual CD-based chiral selector systems for the analysis of a suite of five SRMs with the aim of achieving enantioselective separations [8].…”

Section: (1r2s)-(-)-ephedrine [(-)-Ep] (1s2s)-(1)-pseudoephedrine mentioning

confidence: 99%

Simultaneous chiral separation of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant II: Application in dietary supplements

2007

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Chiral MEKC-MS method was utilized for separation, identification, and quantitation of ten enantiomers of ephedrine and related compounds. Enantioselective separations of all ephedrine alkaloids were accomplished through a combination of polysodium N-undecenoxycarbonyl-L-leucinate (poly-L-SUCL) with 30% v/v ACN. Interestingly, the more hydrophilic stereoisomers were eluted later than the hydrophobic ones indicating that hydrogen bonding interactions are much stronger than hydrophobic interactions in the presence of ACN in chiral MEKC. The method was validated in terms of linearity, LOD, LOQ, precision and robustness. The method was finally used in the analysis of three standard reference materials (SRMs). Results of (-)-ephedrine ranged from 12.49 to 0.24 mg/g, for (+)-pseudoephedrine from 4.04 to 0.019 mg/g, for (-)-norephedrine from 0.36 to 0.0031 mg/g, for (+)-norpseudoephedrine from 0.68 to 0.0052 mg/g, for (-)-methylephedrine from 1.18 to 0.0092 mg/g and for (+)-methylpseudoephedrine from 0.086 to 0.00037 mg/g in the SRMs.

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“…1. Increasing concern on the part of both consumers and regulatory agencies as to the safety of ephedra alkaloids has led to the development of several methods to measure ME by GC (Van Eenoo et al, 2001) and CE Sander et al, 2005) or PSE by GC (Lee et al, 2006), HPLC (Aljazaf et al, 2003;Ishida et al, 2008;Karakus et al, 2008;Ma et al, 2007) and CE . The rapid and sensitive determination and pharmacokinetics study of ME and PSE are important in biochemical and clinical analysis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of methylephedrine and pseudoephedrine in human urine by CE with electrochemiluminescence detection and its application to pharmacokeinetics

Liu

Tian

Jia

et al. 2009

Biomedical Chromatography

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A novel method for the determination of ephedra alkaloids (methylephedrine and pseudoephedrine) was developed by electrophoresis capillary (CE) separation and electrochemiluminesence detection (ECL). The use of ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIMBF(4)) improved the detection sensitivity markedly. The conditions for CE separation, ECL detection and effect of ionic liquid were investigated in detail. The two ephedra alkaloids with very similar structures were well separated and detected under the optimum conditions. The limits of detection (signal-to-noise ratio = 3) in standard solution were 1.8 x 10(-8) mol/L for methylephedrine (ME) and 9.2 x 10(-9) mol/L for pseudoephedrine (PSE). The limits of quantitation (signal-to-noise ratio = 10) in human urine samples were 2.6 x 10(-7) mol/L for ME and 3.6 x 10(-7 )mol/L for PSE. The recoveries of two alkaloids at three different concentration levels in human urine samples were between 81.7 and 105.0%. The proposed method was successfully applied to the determination of ME and PSE in human urine and the monitoring of pharmacokinetics for PSE. The proposed method has potential in therapeutic drug monitoring and clinical analysis.

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Determination of Ephedrine Alkaloids in Dietary Supplement Standard Reference Materials

Cited by 51 publications

References 15 publications

Quality control of herbal medicines by capillary electrophoresis: Potential, requirements and applications

Quality control of herbal medicines by capillary electrophoresis: Potential, requirements and applications

Simultaneous chiral separation of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant II: Application in dietary supplements

Simultaneous determination of methylephedrine and pseudoephedrine in human urine by CE with electrochemiluminescence detection and its application to pharmacokeinetics

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