Determination of endotoxin through an aptamer-based impedance biosensor

Su, Wenqiong; Lin, Meng; Lee, Hyuck; Cho, MiSuk; Choe, Wonyoung; Lee, Youngkwan

doi:10.1016/j.bios.2011.11.009

Cited by 103 publications

(63 citation statements)

References 36 publications

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“…There have been reports of aptamers for food safety, capable of the detection for biotoxins such as the mycotoxins ochratoxin A [27] and fumonisin B [28] and endotoxin [29], for a range of antibiotics with detection limits ranging from nanomolar to micromolar, e.g. Kanamycin A [30] and B [31], neomycin [32], tetracycline [33], chloramphenicol [34], as well as for various bacterial pathogens, including Salmonella typhimurium [35], Escherichia coli O157:H7 [36] Listeria monocytogenes [37] and Staphylococcus aureus [38].…”

Section: Introductionmentioning

confidence: 99%

DNA Aptamers against the Lup an 1 Food Allergen

et al. 2012

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Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases in length, containing a randomised sequence of 49 bases. ß-conglutin was purified from lupin flour and chemically crosslinked to carboxylated magnetic beads. Peptide mass fingerprinting was used to confirm the presence of the ß-conglutin. Single stranded DNA was generated from the randomised pool using T7 Gene 6 Exonuclease and was subsequently incubated with the magnetic beads and the captured DNA was released and amplified prior to a further round of Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Evolution was monitored using enzyme linked oligonucleotide assay and surface plasmon resonance. Once a plateau in evolution was reached, the isolated DNA sequences were cloned and sequenced. The consensus motif was identified via alignment of the sequences and the affinities of these sequences for immobilised ß-conglutin were determined using surface plasmon resonance. The selected aptamer was demonstrated to be highly specific, showing no cross-reactivity with other flour ingredients or with other conglutin fractions of lupin. The secondary structures of the selected aptamers were predicted using m-fold. Finally, the functionality of the selected aptamers was demonstrated using a competitive assay for the quantitative detection of ß-conglutin. . Future work will focus on structure elucidation and truncation of the selected sequences to generate a smaller aptamer for application to the analysis of the Lup an 1 allergen in foodstuffs.

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Section: Introductionmentioning

confidence: 99%

DNA Aptamers against the Lup an 1 Food Allergen

et al. 2012

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“…These toxins are one of the major causes of mortality in intensive care patients due to the absence of specific therapeutics targeting LPS. Endotoxins are highly immunogenic molecules and are one of the major causes of mortality in intensive care patients due to the absence of specific therapeutics targeting LPS (Can et al, 2013;Davies and Cohen, 2011;Su et al, 2012). Medical solutions such as the dialysate and recombinant vaccines can be contaminated with endotoxins and trigger side effects such as tissue injury, endotoxic shock and death (Beutler and Rietschel, 2003;Melia and Saha, 2014).…”

Section: Introductionmentioning

confidence: 99%

In silico designed nanoMIP based optical sensor for endotoxins monitoring

Abdin

Altıntaş

Tothill

2015

Biosensors and Bioelectronics

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“…However, it was requiring a rather long and complicated assay procedure primarily due to its enzymatic reaction necessary for clotting, and the assay results were susceptible to the presence of protease and/or impurities when being applied to actual cell lysates or complicated biological solutions [17]. An LPS of E. coli at a concentration of 10 ng/mL was detected as being based on wave fiber-optic biosensor [18].…”

Section: Introductionmentioning

confidence: 99%