In silico designed nanoMIP based optical sensor for endotoxins monitoring

Abdin, Mohammed J.; Altıntaş, Zeynep; Tothill, Ibtisam E.

doi:10.1016/j.bios.2014.08.009

Cited by 77 publications

(55 citation statements)

References 24 publications

(25 reference statements)

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“…MIPs were produced using the glass beads as a solidphase support for the template (Abdin et al, 2015;Altintas et al, 2015b). In 98 mL of double-distilled water, the following components were added; 39 g N-isopropylacrylamide, 2.2 mL acrylic acid, 2 mg N,N′-methylenebisacrylamide, 33 mg N-tert-butylacrylamide, dissolved in 2 mL ethanol, 5.4 mg N-(3-aminopropyl) methacrylamide hydrochloride.…”

Section: Plastic Antibody Synthesis and Characterisationmentioning

confidence: 99%

Comparative investigations for adenovirus recognition and quantification: Plastic or natural antibodies?

Altıntaş

Pocock

Thompson

et al. 2015

Biosensors and Bioelectronics

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Section: Plastic Antibody Synthesis and Characterisationmentioning

confidence: 99%

Comparative investigations for adenovirus recognition and quantification: Plastic or natural antibodies?

Altıntaş

Pocock

Thompson

et al. 2015

Biosensors and Bioelectronics

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“…Targets that are either scarce, unstable or toxic are not useful as templates and here we describe the rational behind the selection of the target substructure. Given the amphiphilic nature of the target, recognition driven by combined hydrophobic and polar interactions is expected to be particularly powerful1011121314. Phosphatidic acid is a phosphomonoester and the common motif of the glycerophospho lipids.…”

mentioning

confidence: 99%

Phospholipid imprinted polymers as selective endotoxin scavengers

Šulc

Székely

Shinde

et al. 2017

Sci Rep

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Herein we explore phospholipid imprinting as a means to design receptors for complex glycolipids comprising the toxic lipopolysaccharide endotoxin. A series of polymerizable bis-imidazolium and urea hosts were evaluated as cationic and neutral hosts for phosphates and phosphonates, the latter used as mimics of the phospholipid head groups. The bis-imidazolium hosts interacted with the guests in a cooperative manner leading to the presence of tight and well defined 1:2 ternary complexes. Optimized monomer combinations were subsequently used for imprinting of phosphatidic acid as an endotoxin dummy template. Presence of the aforementioned ternary complexes during polymerization resulted in imprinting of lipid dimers – the latter believed to crudely mimic the endotoxin Lipid A motif. The polymers were characterized with respect to template rebinding, binding affinity, capacity and common structural properties, leading to the identification of polymers which were thereafter subjected to an industrially validated endotoxin removal test. Two of the polymers were capable of removing endotoxin down to levels well below the accepted threshold (0.005 EU/mg API) in pharmaceutical production.

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“…Here, we have developed a self-contained, on-chip peristaltic pump using iron filings and PDMS that is driven by magnets and a motor. We have used this magnetic pump to build a diafiltration-on-chip system that can be used to rapidly purify biologically-relevant samples, including small molecules from beads 20–22 and beads from cells 23,24 . However, by replacing the membrane filter, the use of the diafiltration-on-chip device may also be extended to include size-based purification of other samples such as extracellular vesicles 25 , proteins 26 , and nanoparticles 3 .…”

Section: Discussionmentioning

confidence: 99%

Microfluidic diafiltration-on-chip using an integrated magnetic peristaltic micropump

et al. 2017

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Diafiltration is a membrane filtration technique that rapidly removes permeable molecules from a solution by controlling the tangential and orthogonal flows over a membrane and by replenishing the permeate with an equivalent amount of replacement buffer. However, its application to the purification of many key biomaterials and nanomaterials has been limited by the large dead volume (>10 mL) that is required to automate the process. To address this challenge, we have developed a diafiltration-on-a-chip device that can process low-volume samples (50 μL). The key innovation of this device is a magnetically-driven on-chip peristaltic pump that is able to continuously drive fluid flow at rates as high as 50 mL/hr with minimal external instrumentation and a dead volume of <50 μL. To demonstrate the utility of this device, we purified microbeads from dye with >99% purity and >96% retention within two hours. We additionally showed that cells could be purified from microbeads with >97% purity and >97% retention in two hours. Because our approach requires minimal instrumentation, it is well suited for on-chip parallelization, which was demonstrated by incorporating four complete diafiltration systems onto a single credit card-sized chip.

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In silico designed nanoMIP based optical sensor for endotoxins monitoring

Cited by 77 publications

References 24 publications

Comparative investigations for adenovirus recognition and quantification: Plastic or natural antibodies?

Comparative investigations for adenovirus recognition and quantification: Plastic or natural antibodies?

Phospholipid imprinted polymers as selective endotoxin scavengers

Microfluidic diafiltration-on-chip using an integrated magnetic peristaltic micropump

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